Up-regulated autophagy: as a protective factor in adipose tissue of WOKW rats with metabolic syndrome
Diabetology & Metabolic Syndrome volume 10, Article number: 13 (2018)
Wistar Ottawa Karlsburg W (RT1u) rats (WOKW) are a model of the metabolic syndrome (MetS). Adipose tissue (AT) and peripheral nerves of WOKW rats exhibit up-regulated autophagy and inflammation corresponding with decreased apoptosis rate. The aim of this study was to characterize AT in WOKW rats in relation to autophagic activity.
mRNA and protein expression of adiponectin, pro-inflammatory and pro-apoptotic markers including MCP1, TNFα, cleaved caspase-3 and RNF157, a new candidate gene regulated through autophagy, were analyzed in adipocytes isolated from visceral and subcutaneous AT of 5-month old WOKW rats with MetS and LEW.1W controls in response to pharmacological inhibition of autophagy. Immunohistochemistry was performed to detect adiponectin and RNF157 protein in cultured adipocytes.
Inhibition of autophagy by LY294002 was associated with a fourfold up-regulation of adiponectin expression and a decrease of RNF157 protein and pro-inflammatory markers—MCP-1 and TNFα predominantly in visceral adipocytes of obese WOKW rats compared to LEW.1W rats. Moreover, inhibition of autophagic activity correlates with an activation of cleaved caspase-3 apoptotic signaling pathway.
Up-regulated autophagy in obese WOKW rats contributes to the regulation of visceral AT function and involves an altered balance between pro-inflammatory and protective adipokine expression. Our data suggest that activation of AT autophagy protects against adipocyte apoptosis at least under conditions of obesity related MetS in WOKW rats.
The metabolic syndrome (MetS) characterized by obesity, hypertension, hyperinsulinemia, dyslipidemia and impaired glucose metabolism represents a risk factor for cardiovascular diseases and type 2 diabetes. In addition, a pro-inflammatory state may contribute to diabetic peripheral neuropathy, nephropathy, retinopathy and macrovascular complications [1,2,3,4,5,6].
Inflammation of adipose tissue (AT)—a symptom of AT dysfunction both in humans and model organisms which develops with weight gain and diabetes—is associated with AT autophagic activity [7,8,9]. Autophagy is an essential, lysosomal degradation process, which is implicated in cellular homeostasis through break down and recycling of aggregated proteins and damaged organelles . However, activated autophagy has been generally considered as a cell-protection mechanism, which promotes cell survival; the excessive activation of autophagy may lead to autophagic cell death .
Autophagy has been shown to prevent or induce inflammatory responses and to regulate of cytokine production in AT of obesity in humans and animal models [12,13,14]. The molecular mechanisms underlying the interaction between altered immune-response and autophagy regulation in obesity are still poor investigated.
Recently, we have shown that enhanced autophagy characterized by up-regulated expression of autophagy genes in AT and peripheral nerves occurs in obese Wistar Ottawa Karlsburg W (RT1u) (WOKW) rats, a model for MetS [15, 16]. WOKW rats with the MHC RT1u haplotype develop a polygenetically inherited metabolic syndrome with obesity, hypertension, dyslipidemia and hyperinsulinemia, closely reflecting the human MetS [17,18,19]. Up-regulation of autophagy in WOKW rats corresponded with macrophage infiltration in the sciatic nerves and AT without signs of neuropathy or diabetes. Moreover, activated autophagy seems to protect from caspase-3 mediated apoptosis in AT and nerves of WOKW rats [15, 16]. There is strong evidence for a causal relationship between autophagy and AT function in obesity.
Here, we tested the hypothesis that autophagic activity contributes to the regulation of protective, inflammatory and apoptotic factors in adipocytes isolated from visceral and subcutaneous AT of WOKW rats. We provide data suggesting that AT autophagy represents a key mechanism protecting against the development of obesity-related metabolic comorbidities.
Five-month old WOKW (n = 12) and LEW.1W (n = 12) male rats were bred and kept under standard conditions maintained at 21 ± 1 °C on a 12:12 h light/dark cycle (5 a.m./5 p.m.) at the Saxon Incubator of Clinical Translation (SIKT, Leipzig, Germany). All rats had free access to water and were fed with a regular chow food (Sniff, Soest, Germany). Body weight was recorded for each group (WOKW mean 673 g, p < 0.05; LEW.1W mean 503 g, p < 0.05).
Experiments followed the international guidelines of animal care and the study protocols were approved by the Landesdirektion Leipzig, the local authority for animal care (T01/13 and T08/16). All rats were sacrificed via an isoflurane overdose at the end of the experiment.
At an age of 5 months all animals were weighed, blood was collected and whole body composition (fat mass, lean mass and total body water) was determined in awake rats by using nuclear magnetic resonance technology with EchoMRI700™ instrument (Echo Medical Systems, Houston, TX, USA). Data were analyzed by the manufacturer’s software.
Serum insulin concentrations were measured by ELISA using Mercodia Rat ELISA (Uppsala, Sweden). Blood glucose values were determined from whole venous blood samples using an automated glucose monitor (FreeStyle mini, Abbott GmbH, Ludwigshafen, Germany). HbA1c levels were analysed by an automatic chemical analyzer in our Institute of Laboratory Medicine and Clinical Chemistry.
Primary adipocyte cell cultures
WOKW and LEW.1W rats were anesthetized with 2.5% isoflurane inhalation. Subcutaneous and visceral adipose tissue depots were removed under sterile conditions, washed in PBS and incubated in a 5 ml of preadipocyte isolation buffer containing 123 mM NaCl, 5 mM KCl, 1.3 mM CaCl2, 5 mM Glucose, 100 mM HEPES, 4% BSA (bovine serum albumin fraction V) and 0.2% collagenase (type 1). Incubations were performed in a water bath with rotating flasks (120 rpm) at the temperature 37 °C for 40 min. After incubation the tissue remnants and cell aggregates were removed by filtration through a 100 µm nylon screen. The cells passing the filter were pelleted by centrifugation (5 min, 1500 rpm at RT) and then resuspended in an erythrocyte lysis buffer containing 0.154 M NH4Cl, 0.01 M KHCO3 and 0.1 mM EDTA for 10 min at RT. After incubation the preadipocyte pellet was resuspended and maintained in high-glucose Dulbecco’s modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Sigma) at 37 °C in a 5% CO2 atmosphere. Approximately 20,000 cells per well of a 24-well plate or per 30-mm culture dishes were obtained.
At day 2 of confluence, preadipocytes were induced to differentiate into adipocytes by using medium with 1 mM insulin, 0.4 mg/ml dexamethasone and 0.5 mM 3-isobutyl-1-methylxanthine changed every 48 h. After additional 7 days, about 85% of the cells had accumulated large-sized fat droplets as verified by phase contrast microscopy. For studies of autophagy dependent regulation of adiponectin, MCP-1, TNFα, RNF157 and cleaved caspase-3 expression, adipocytes were used at day 9 after the induction of differentiation.
Inhibition of the PI3K pathway
Phosphorylation of phosphatidylinositol 3-kinase (PI3K) is required for autophagy activation, synthesis of autophagosomes and lipidation of cytosolic LC3-I into the active, autophagosome membrane-bound form, LC3-II. LY294002 is a specific inhibitor of PI3K phosphorylation .
Dose-dependent inhibitory effect of LY294002 on PI3K phosphorylation was studied by treating adipocyte cells with increasing concentrations of LY294002 (20, 30 and 50 µM) in serum-free medium for 48 h. Completely inhibited phosphorylation was obtained at the concentration of 50 µM. To inhibit autophagy process, visceral and subcutaneous adipocytes were incubated in serum-free DMEM medium at 37 °C in the presence or the absence of LY294002 at a concentration of 50 µM for 48 h (Fig. 1a).
Visceral and subcutaneous adipocyte cultures of WOKW and LEW.1W rats (total number = 32; n = 4, per group) were fixed with 2% formaldehyde in 0.1 M PBS and 5% sucrose at 37 °C for 15 min. For detection of adiponectin and LC3 protein expression in adipocytes, double staining was performed using rabbit polyclonal anti-perilipin A (1 µg/ml; ab3526; Abcam, Cambridge, MA) and mouse monoclonal anti-adiponectin (2 µg/ml; ab22554; Abcam) or mouse anti-LC3 (2 μg/ml; clone 5F10; nanoTools, Munich, Germany) antibodies at 4 °C overnight. For detection of RNF157 (ring finger protein 157) protein expression in cultivated adipocytes a single staining with a rabbit polyclonal anti-RNF157 antibody (2 µg/ml; bs-9226R; Bioss, antibodies-online GmbH, Germany) was conducted. After rinsing with buffer, CY3-conjugated goat anti-mouse and/or FITC conjugated goat anti-rabbit antibodies (2 μg/ml; Dianova, Hamburg, Germany) were applied at room temperature (RT) for another 2 h. The coverslips were mounted with Glycerol (DAKO, Hamburg, Germany) containing DAPI 10 μg/ml (Serva, Heidelberg, Germany) for nuclear staining and DABCO 25 μg/ml (Sigma Aldrich) to prevent photobleaching. By replacement of the primary antibody with normal mouse IgG or PBS, respectively, no specific immunoreaction occurred.
All immunostaining images were taken using a Zeiss LSM 700 confocal microscope, and the ZEN 2011 software. The following laser settings were used: scan speed-9, pixel dwell 1.58 μs, scan time 11.62 s, number of scans-4, Bit depth-8 bit, DAPI track-laser (405 nm, pinhole-3.22 airy units, gain-766), FITC track-laser (488 nm, pinhole 1.90 airy units, gain-733) and CY3 track-laser (555 nm, pinhole 1.70 airy units, gain-633).
For preparing cell cultures lysates, cultured adipocytes (total number = 24; n = 6, per group) were washed in PBS and homogenized in lysis buffer containing 50 nM Tris, 150 nM NaCl, 1% NP-40, 0.5% Na-deoxycholate and 0.1% SDS. Samples were then incubated over 1 h in ice and centrifuged at 13,000×g for 15 min at 4 °C. Protein concentration was assessed with the BCA protein assay (Pierbo Science, Bonn, Germany). Proteins (20 μg per lane) were separated by electrophoresis on 10 or 15% SDS–polyacrylamide gels and transferred to nitrocellulose membranes by electroblotting. Nonspecific binding sites were blocked with 5% dry milk for 50 min. The blots were incubated with different antibodies at 4 °C overnight: mouse anti-LC3 (0.5 μg/ml; clone 2G6; nanoTools, Munich, Germany), mouse anti-TNF alpha (tumor necrosis factor alpha; 0.5 μg/ml; ab6671; Abcam, MA, USA), rabbit polyclonal anti-MCP1 (monocyte chemoattractant protein-1; 2 µg/ml; ab25124; Abcam), mouse monoclonal anti-adiponectin (2 µg/ml; ab22554; Abcam), rabbit polyclonal anti-RNF157 (1 µg/ml; bs-9226R; Bioss, antibodies-online GmbH, Germany), rabbit anti-cleaved caspase-3, Atg5 (D1G9), anti-mTOR, rabbit anti-Akt, mouse anti-pAkt, rabbit anti-PI3K, mouse anti-pPI3K (1 μg/ml; Cell Signaling Technology, Danvers, USA). Proteins were detected by incubating with HRP conjugated secondary antibodies (0.5 μg/ml; Dianova) at RT for 2 h and chemiluminescence kit (Amersham, Pharmacia, Freiburg, Germany). Integrated optical densities of the immunoreactive protein bands were measured with Gel Analyzer software (Media Cybernetics, Silver Spring, MD). Equal protein loading was verified using mouse anti-d-glyceraldehyde-3-phosphate dehydrogenase antibody (GAPDH; 0.2 μg/ml; Research Diagnostics, Flanders, The Netherlands). The extract of Jurkat cells with cytochrome c-induced apoptosis (10 μg; Cell Signaling Technology) was used as positive control for the detection of cleaved caspase-3.
For mRNA analyses, subcutaneous and visceral AT depots of WOKW and LEW.1W rats (n = 4) were removed under 2.5% isoflurane anesthesia, transferred on ice, rinsed in PBS and immediately frozen in liquid nitrogen. mRNA expression of rat marker genes of Rnf157 and Mcp-1 was measured by quantitative real-time RT-PCR in a fluorescent temperature cycler using the TaqMan assay, and fluorescence was detected on an ABI-PRISM 7000 sequence detector (Applied Biosystems, Darmstadt, Germany). Total RNA was isolated using TRIzol (Life Technologies, Grand Island, NY). 1 μg RNA was reverse transcribed with standard reagents (Life Technologies). From each RT-PCR consisting of an initial denaturation at 95 °C for 10 min, followed by 40 PCR cycles, each cycle consisting of 95 °C for 15 s, 60 °C for 1 min, and 72 °C for 1 min. 2 μl was amplified in a 20-μl PCR using the Brilliant SYBR Green PCR mix from Thermo Fisher Scientific (Waltham, MA, USA). The following primers were used: rat Rnf157, 5′-AACAGCCAAGGGCTCAAACT-3′ (sense) and 5′-TCTGACTCACTGCAAGAGCG-3′ (antisense); rat Mcp-1, 5′-TAGCATCCACGTGCTGTCTC-3′ (sense) and 5′-CAGCCGACTCATTGGGATCA-3′ (antisense) (Biomers, Ulm, Germany). Rat Rnf157 and Mcp-1 mRNA expression was calculated relative to the mRNA expression of 18S rRNA, determined by a premixed assay on demand for human 18S rRNA (Hs99999901_s1; Life Technologies, CA). Amplification of specific transcripts was confirmed by melting curve profiles (cooling the sample to 60 °C and heating slowly to 95 °C with measurement of fluorescence) at the end of each PCR. Specificity of PCR was verified by subjecting the amplification products to agarose gel electrophoresis, which verified the presence of a single band of appropriate molecular weight for each amplification product.
Data are presented as mean ± SEM (Figs. 1, 3, 4, 5, 7). Differences between the groups were validated by one-way-ANOVA and the Newman–Keuls test using SigmaStat (Jandel Scientific, San Rafael, CA). A value of p < 0.05 was considered statistically significant.
Phenotypic characterization of WOKW rats
The phenotype of the 5-months-old WOKW (n = 24) and LEW.1W control rats (n = 24) is summarized in Table 1. WOKW rats have a significantly higher body weight as well as fasting serum insulin concentrations compared to healthy LEW.1W rats. In contrast, HbA1c levels and fasted blood glucose did not differ between both strains. The adiposity index was significantly increased in WOKW compared to LEW.1W rats (Table 1).
Inhibition of autophagy in visceral and subcutaneous adipocyte cell cultures of WOKW and LEW.1W rats
We have previously shown the strikingly up-regulated autophagy in adipose depots of obese WOKW rats with metabolic syndrome in correlation with inhibition of caspase-3 apoptotic signal pathway .
Here, we extent these findings by pharmacologically inhibiting autophagy in cultured adipocytes of obese WOKW rats with MetS and in lean LEW.1W control healthy rats. Mature visceral and subcutaneous adipocytes from both strains have been inhibited with an autophagy inhibitor—LY294002 at the concentration of 50 µM for 48 h.
As shown in Fig. 1, phosphorylation of key autophagy signaling pathway—PI3K p85 and Akt protein in visceral and subcutaneous adipocytes of WOKW and LEW.1W rats was inhibited with LY294002 inhibitor after 45 min of treatment. Next, we assessed the protein level of the autophagy related genes—Atg5/12, LC3 and mTOR in adipocyte cell cultures after inhibition.
Visceral adipocyte cell cultures of WOKW and LEW.1W rats display a significant decrease of LC3 immunoreactivity compared to the non-inhibited control cultures after inhibition with LY294002 for 48 h (Fig. 2a–d).
Western blot analyses confirmed an inhibitory effect of LY294002. Expression of Atg5/12 protein was decreased about threefold in visceral adipocytes of WOKW rats and 1.3-fold in LEW.1W rats (Fig. 3a, b). LC3-II (membrane associated, 16 kDa form of LC3) displayed a 2.6-fold down-regulation in visceral adipocytes of WOKW rats and a 3.7-fold decrease in LEW.1W rats. In subcutaneous adipocytes, a 1.6-fold down-regulation of LC3 protein expression was found in WOKW rats and 4.5-fold decrease in LEW.1W control rats (Fig. 3a, c). Expression of mTOR was increased about 1.6-fold in visceral adipocytes of both strains. Contrary, in subcutaneous adipocyte cell cultures only LEW.1W rats show higher expression of mTOR after autophagy inhibition (Fig. 3a, d).
Autophagy dependent regulation of adiponectin, MCP-1 and TNFα protein expression in cultured adipocytes of WOKW and LEW.1W rats
In the obese state, the adipocytes increase secretion of pro-inflammatory chemokines and cytokines, such as MCP-1, TNFα or interleukins (ILs) which are involved in insulin resistance [21,22,23]. Moreover, the expression of the insulin-sensitizing adipokine—adiponectin is strikingly down-regulated in obesity . AT protein level of adiponectin correlates with up-regulated autophagy and was lower in cultured adipocytes of obese WOKW rats as compared to LEW.1W control rats.
After autophagy inhibition (48 h), adiponectin showed an about 3.5- and 4-fold (WOKW rats) and 1.2- and 3.6-fold (LEW.1W rats) up-regulation in visceral and subcutaneous adipocyte cell cultures (Fig. 4a, b).
Moreover, we confirm the up-regulated mRNA (Additional file 1: Figure S1) and protein expression of MCP-1 in adipose depots of WOKW rats in comparison to LEW.1W control rats mainly in visceral adipocytes (Fig. 4a, c). MCP-1 protein expression was decreased in visceral adipocytes of both strains after LY294002 treatment. In contrast, MCP-1 protein in subcutaneous adipocytes is down-regulated in LEW.1W rats only (Fig. 4a, c).
Additionally, we have found a fourfold increase in TNFα protein expression in visceral adipocyte cell culture of WOKW rats, which could be significantly attenuated after autophagy inhibition. The expression of TNFα in subcutaneous adipocytes has been detected at comparable levels between all experimental groups (Fig. 4a, d).
As shown in Fig. 5a–d immunohistofluorescence analysis of adiponectin distribution in visceral adipocyte cell cultures demonstrating an increase of adiponectin immunoreactivity after LY294002 treatment. Immunofluorescence of adiponectin in subcutaneous adipocytes was rarely detectable.
Regulation of RNF157 in adipocyte cell cultures in obese WOKW rats
Unexpectedly, a strikingly high mRNA expression of new candidate gene—Rnf157, a neuroprotective protein , has been detected in visceral and subcutaneous depots of WOKW rats and in visceral fat of LEW.1W rats (Fig. 6a–f).
Interestingly, we found a 5- and 2.6-fold decrease of RNF157 protein expression after autophagy inhibition in visceral adipocyte cell cultures of WOKW and LEW.1W rats, respectively. Subcutaneous adipocytes of WOKW rats exhibit a 1.4-fold decrease in RNF157 expression after treatment. No changes in RNF157 expression were observed in subcutaneous adipocyte cultures of LEW.1W rats after autophagy inhibition (Fig. 6f). Inhibition of autophagy resulted in decrease of RNF157 immunoreactivity in visceral adipocytes of both strains (Fig. 6b–e).
Up-regulation of cleaved caspase-3 signal pathway after inhibition of autophagic activity
Since autophagy promotes survival or once exhausted could activate apoptosis , cleaved caspase-3, an apoptosis marker, has been assessed in cultivated adipocytes in response to autophagy inhibition. Western blot analysis demonstrate significantly increased cleaved caspase-3 (17 kDa subunit) protein expression after autophagy inhibition in both adipocyte cell cultures of WOKW and LEW.1W rats predominantly in visceral adipocytes (Fig. 7a–c).
Obesity and metabolic syndrome (MetS) have been linked with enhanced autophagic activity in AT . Increased expression of autophagy genes correlates with visceral fat distribution, adipocyte hypertrophy, secretion of pro-inflammatory adipokine and contributes to adipose tissue (AT) inflammation [7, 27, 28]. There is a complex bidirectional relationship between autophagic activity and inflammation. Autophagy pathway induces or suppresses inflammatory responses, whereas inflammatory signals regulate the autophagic flux [12, 29, 30]. Inflammatory activation in obesity occurs principally in metabolic tissues including AT, liver and pancreas . Expansion of AT with predominant adipocyte hypertrophy triggers the secretion of pro-inflammatory chemokines and causes obesity-induced inflammation. Among them, MCP-1, TNFα, interleukin IL-1 and IL-6 have been reported to promote obesity related-insulin resistance .
Obesity-related AT inflammation and insulin resistance result in a decrease of anti-inflammatory adipokines, such as adiponectin [28, 31, 32]. Slutsky, Rudich et al. have reported the association between decreased adiponectin and elevated autophagy in omental AT of patients with obesity . However, obesity-related inflammation with up-regulated autophagy has been assumed as a destructive factor, the impact of autophagy as a protective mechanism in obesity is carefully discussed. Codogno and Meijer argued that initially obesity-induced insulin resistance functions as an adaptive mechanism to increase autophagy in order to protect cells against death . Activated autophagy characterized by an increased expression of autophagy genes ATG5, LC3A, and LC3B as well as elevated autophagic flux in omental and subcutaneous adipose tissue has been reported in non-diabetic obese individuals more pronounced as in type 2 diabetes (T2D) patients [7, 9, 35]. Our previous data confirmed that autophagy contributes to AT inflammation in obesity and its related metabolic comorbidities [7, 9, 15, 16]. Increased autophagy in obese and T2D patients compared to lean individuals is reflected by a higher infiltration of Iba-1 positive macrophages and up-regulation of TNFα and IL-6 expression in visceral AT. Interestingly, increased autophagy and inflammation in visceral AT of obese patients without T2D corresponded with inactivation of cleaved-caspase 3 dependent apoptosis pathway . Autophagy occurring parallel to inflammation is assumed as a protective factor, since it does not invoke apoptosis. Correspondingly, we found a significant increased autophagy with macrophage infiltration in the sciatic nerves and visceral AT of adult, obese WOKW rats with MetS [15, 16]. Notably, WOKW rats do not develop any age-related MetS complications such as diabetes or neuropathy. Therefore, we proposed that up-regulated autophagy exerts a protective role against development of diabetes and peripheral neurodegeneration in the WOKW rat model.
The present study highlights the impact of autophagy on adipocyte function in cultured adipocytes of obese WOKW rats with MetS. We demonstrate that inhibition of autophagy leads to enhanced expression of protective and inflammatory factors and induces the expression of cleaved caspase-3 apoptotic marker in adipocytes of WOKW rats as compared to the lean, healthy control LEW.1W rats.
In accordance with our previous findings, significantly up-regulated autophagy has been detected in cultured adipocytes of obese WOKW rats as compared to LEW.1W. We found a markedly higher expression of autophagic molecular markers, including Atg5-Atg12 complex, lipidated/cleaved form of LC3 (LC3-II), and down-regulation of mTOR protein, respectively.
Up-regulated autophagy in visceral adipocytes of WOKW rats vs. LEW.1W corresponds with an increase of RNF157 protein expression. RNF157 could be co-regulated by autophagy mechanisms in adipose tissue or induce autophagy. Significantly higher mRNA and protein levels of RNF157 (about threefold) correlate with visceral AT mass, obesity and MetS parameters (WOKW vs. LEW.1W). Importantly, an inhibition of autophagic activity reduced the expression of RNF157 protein in AT of both strains. RNF157 is predominantly expressed in the brain, whereas non-neuronal tissues show little expression. This protein belongs to E3 ubiquitin RING ligases and is implicated in regulation of neuronal survival and morphology in cultured neurons . In addition, a downstream component and substrate of RNF157—the adaptor protein Fe65 was found to trigger neuronal apoptosis. These unexpected observations of AT specific RNF157 expression and its correlation with activated autophagy require further mechanistic studies.
Among adipokines, adiponectin is an important modulator of glucose metabolism involved in insulin sensitivity and secretion . Moreover, based on its anti-inflammatory properties, adiponectin has been classified as a protective adipokine . Recently, up-regulated autophagy has been show to be implicated in impaired adiponectin secretion in omental AT of patients with obesity . We have previously reported a tenfold reduced adiponectin mRNA in AT of obese WOKW rats as compared to healthy controls . The present study revealed that autophagy significantly contributes to the regulation of adiponectin secretion in visceral and subcutaneous AT. Notably, lower adiponectin levels with up-regulated autophagy in cultured adipocytes (WOKW vs. LEW.1W) have been increased about fourfold after autophagy inhibition in adipocytes of WOKW rats. Whether the up-regulation of autophagy genes with attenuated expression of adiponectin in AT of WOKW rats mediates anti-metabolic or protective actions is questionable. The WOKW rats develop complete metabolic syndrome, but are resistant to MetS complications.
In order to investigate autophagy dependent regulation of pro-inflammatory cytokines, we focused particularly on MCP-1 and TNF-α mRNA and protein expression in AT and cultivated adipocytes of WOKW rats with MetS and LEW.1W control healthy rats. MCP-1, a member of the chemokine family, is a crucial factor implicated in chronic inflammation of visceral AT. An increased level of MCP-1 in obesity links to accumulation of M1-proinflammatory macrophages in visceral AT . MCP-1 plays a crucial role in the recruitment of monocytes and T lymphocytes into tissues . The resident stromal cells, monocytes, endothelial cells, preadipocytes and adipocytes have been determined to MCP-1 expression. More recently, adipocyte progenitor cells have been identified as the initial cellular source of MCP-1 . A potentially direct action of MCP-1 on autophagy is unknown. In the present study, we confirmed that MCP-1 expression in visceral adipocytes is directly regulated through autophagic activity. The MCP-1 mRNA levels were significantly higher in visceral and subcutaneous AT of WOKW rats as compared with LEW.1W rats. Correspondingly, higher MCP-1 protein expression was detected in cultured visceral adipocytes of WOKW rats vs. LEW.1W rats. The inhibition of autophagy resulted in significantly decrease of MCP-1 expression in visceral adipocytes of both strains. Differences between MCP-1 expression in AT (mRNA level) and in cultivated adipocytes (protein level) in WOKW rats vs. LEW.1W rats can be explained by the rare occurrence of macrophages or other components of stromal-vascular fraction in cell cultures as a source of MCP-1.
MCP-1, in concert with other pro-inflammatory cytokines such as TNF-α or IL-6 overexpressed by adipocytes in obesity and MetS, leads to insulin sensitivity. In the visceral adipocytes of WOKW rats, up-regulated autophagy corresponds to higher levels of TNFα, whereas the inhibition of autophagic activity significantly decreased TNFα expression. Perhaps, the autophagic process plays a regulatory function of cytokine dependent insulin sensitivity in visceral AT. The activation of autophagy pathways during inflammation has been reported to regulate pathogen clearance, antigen presentation, lymphocyte development and the production of proinflammatory cytokines . The mechanistic relationship between autophagy and pro-inflammatory cytokine activation is dependent on the priority of occurring process, since one of these could precede the second.
In this study we demonstrate that an inhibition of autophagic activity significantly changed the expression of protective but also inflammatory factors and parallel markedly up-regulated the expression of cleaved caspase-3 in cultured adipocytes. However, the occurred changes were more evident in visceral adipocytes of obese WOKW rats than in LEW.1W healthy control rats. These results are compatible with our previous observation that increased autophagic activity protect from caspase-3 mediated apoptosis in AT of obese human and rats [9, 15]. The biological consequences of manipulation of autophagy process are dependent on cell type and metabolic context and can exert opposing effects. It has been assumed that “basal autophagy” as an adipocyte survival mechanism is essential for completion of adipogenesis. Defects in autophagy or its inhibition cause the higher level of apoptosis in AT and cultivated adipocytes (our observation). Notably, the autophagy related protein (Atg)-5 knockout mice die within the first day after birth. Atg-7 knockout mice are insulin-sensitive and resistant to the development of obesity . Contrary, up-regulated autophagy is implicated in the obesity state and obesity-related inflammation. Therefore, autophagy represents distinctly regulated mechanism, which may protect against or develop obesity-associated AT dysfunction.
Based on our previously observations about up-regulated autophagy in visceral AT of obese patients and WOKW rats with MetS in correlation with inflammation but not with apoptosis and T2D, we postulated that autophagy as a key protective rather as destructive factor. Here, we show the consequences of preventing autophagic activity in adipocytes, predominantly in visceral AT of WOKW rats as markedly changes of pro-inflammatory and protective adipokines parallel with activated apoptosis. This fact highlights the autophagy process for future research of diabetes prevention in obesity.
autophagy related protein 5–12
hemoglobin-A1c glycated hemoglobin
microtubule associated protein light chain 3
monocyte chemoattractant protein-1
mammalian target of rapamycin
phosphoinositide 3 kinase
phospho-phosphoinositide 3 kinase
ring finger protein 157
type 2 diabetes
tumor necrosis factor alpha
Wistar Ottawa Karlsburg W
Bonadonna R, Cucinotta D, Fedele D, Riccardi G, Tiengo A. The metabolic syndrome is a risk indicator of microvascular and macrovascular complications in diabetes: results from metascreen, a multicenter diabetes clinic-based survey. Diabetes Care. 2006;29:2701–7.
Van Gaal LF, Mertens IL, De Block CE. Mechanisms linking obesity with cardiovascular disease. Nature. 2006;444:875–80.
Kanneganti TD, Dixit VD. Immunological complications of obesity. Nat Immunol. 2012;13:707–12.
Singleton JR, Smith AG. Therapy insight: neurological complications of prediabetes. Nat Clin Pract Neurol. 2006;2:276–82.
Andersen CJ, Murphy KE, Fernandez ML. Impact of obesity and metabolic syndrome on immunity. Adv Nutr. 2016;7:66–75.
Klöting N, Blüher M. Adipocyte dysfunction, inflammation and metabolic syndrome. Rev Endocr Metab Disord. 2014;15:277–87.
Kovsan J, Blüher M, Tarnovscki T, Klöting N, Kirshtein B, Madar L, et al. Altered autophagy in human adipose tissues in obesity. J Clin Endocrinol Metab. 2011;96:E268–77.
Öst A, Svensson K, Ruishalme I, Brännmark C, Franck N, Krook H, et al. Attenuated mTOR signaling and enhanced autophagy in adipocytes from obese patients with type 2 diabetes. Mol Med. 2010;16:235–46.
Kosacka J, Kern M, Klöting N, Paeschke S, Rudich A, Haim Y, et al. Autophagy in adipose tissue of patients with obesity and type 2 diabetes. Mol Cell Endocrinol. 2015;409:21–32.
Levine B, Klionsky DJ. Development by self-digestion: molecular mechanisms and biological functions of autophagy. Dev Cell. 2004;6:463–77.
Chen ZF, Li YB, Han JY, Wang J, Yin JJ, Li JB, et al. The double-edged effect of autophagy in pancreatic beta cells and diabetes. Autophagy. 2011;7:12–6.
Levine B, Mizushima N, Virgin HW. Autophagy in immunity and inflammation. Nature. 2011;469:323–35.
Wang Y, Li YB, Yin JJ, Wang Y, Zhu LB, Xie GY, et al. Autophagy regulates inflammation following oxidative injury in diabetes. Autophagy. 2013;9:272–7.
Sanderson RD, Elkin M, Rapraeger AC, Ilan N, Vlodavsky I. Heparanase regulation of cancer, autophagy and inflammation: new mechanisms and targets for therapy. FEBS J. 2017;284:42–55.
Kosacka J, Koch K, Gericke M, Nowicki M, Heiker JT, Klöting I, et al. The polygenetically inherited metabolic syndrome of male WOKW rats is associated with enhanced autophagy in adipose tissue. Diabetol Metab Syndr. 2013;5:23.
Kosacka J, Nowicki M, Blüher M, Baum P, Stockinger M, Toyka KV, et al. Increased autophagy in peripheral nerves may protect Wistar Ottawa Karlsburg W rats against neuropathy. Exp Neurol. 2013;250:125–35.
van den Brand J, Kovács P, Klöting I. Features of the metabolic syndrome in the spontaneously hypertriglyceridemic Wistar Ottawa Karlsburg W (RT1u Haplotype) rat. Metabolism. 2000;49:1140–4.
van den Brand J, Kovács P, Klöting I. Metabolic syndrome and aging in Wistar Ottawa Karlsburg W rats. Int J Obes Relat Metab Disord. 2002;26:573–6.
Klöting N, Blüher M, Klöting I. The polygenetically inherited metabolic syndrome of WOKW rats is associated with insulin resistance and altered gene expression in adipose tissue. Diabetes Metab Res Rev. 2006;22:146–54.
Blommaart EF, Krause U, Schellens JP, Vreeling-Sindelárová H, Meijer AJ. The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes. Eur J Biochem. 1997;243:240–6.
Hotamisligil GS, Shargill NS, Spiegelman BM. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science. 1993;259:87–91.
Sartipy P, Loskutoff DJ. Monocyte chemoattractant protein 1 in obesity and insulin resistance. Proc Natl Acad Sci USA. 2003;100:7265–70.
Jorge AS, Jorge GC, Paraíso AF, Franco RM, Vieira LJ, Hilzenderger AM, et al. Brown and white adipose tissue expression of IL6, UCP1 and SIRT1 are associated with alterations in clinical, metabolic and anthropometric parameters in obese humans. Exp Clin Endocrinol Diabetes. 2017;125:163–70.
Kern PA, Di Gregorio GB, Lu T, Rassouli N, Ranganathan G. Adiponectin expression from human adipose tissue: relation to obesity, insulin resistance, and tumor necrosis factor-alpha expression. Diabetes. 2003;52:1779–85.
Matz A, Lee SJ, Schwedhelm-Domeyer N, Zanini D, Holubowska A, Kannan M, et al. Regulation of neuronal survival and morphology by the E3 ubiquitin ligase RNF157. Cell Death Differ. 2015;22:626–42.
Yousefi S, Perozzo R, Schmid I, Ziemiecki A, Schaffner T, Scapozza L, et al. Calpain-mediated cleavage of Atg5 switches autophagy to apoptosis. Nat Cell Biol. 2006;8:1124–32.
Blüher M. Adipose tissue dysfunction contributes to obesity related metabolic diseases. Best Pract Res Clin Endocrinol Metab. 2013;27:163–77.
Jung UJ, Choi MS. Obesity and its metabolic complications: the role of adipokines and the relationship between obesity, inflammation, insulin resistance, dyslipidemia and nonalcoholic fatty liver disease. Int J Mol Sci. 2014;15:6184–223.
Saitoh T, Akira S. Regulation of innate immune responses by autophagy-related proteins. J Cell Biol. 2010;189:925–35.
Levine B, Kroemer G. Autophagy in the pathogenesis of disease. Cell. 2008;132:27–42.
Guilherme A, Virbasius JV, Puri V, Czech MP. Adipocyte dysfunctions linking obesity to insulin resistance and type 2 diabetes. Nat Rev Mol Cell Biol. 2008;9:367–77.
de Luis DA, Pacheco D, Primo D, Izaola O, Aller R. The association of SNP276G>T at adiponectin gene with insulin resistance and circulating adiponectin in morbid obese patients after a biliopancreatic diversion surgery. Obes Surg. 2017. https://doi.org/10.1007/s11695-017-2766-7.
Slutsky N, Vatarescu M, Haim Y, Goldstein N, Kirshtein B, Harman-Boehm I, Gepner Y, Shai I, Bashan N, Blüher M, Rudich A. Decreased adiponectin links elevated adipose tissue autophagy with adipocyte endocrine dysfunction in obesity. Int J Obes. 2016;40:912–20.
Codogno P, Meijer AJ. Autophagy: a potential link between obesity and insulin resistance. Cell Metab. 2010;11:449–51.
Haim Y, Blüher M, Slutsky N, Goldstein N, Klöting N, Harman-Boehm I, et al. Elevated autophagy gene expression in adipose tissue of obese humans: a potential non-cell-cycle-dependent function of E2F1. Autophagy. 2015;11:2074–88.
Ohashi K, Yuasa D, Shibata R, Murohara T, Ouchi N. Adiponectin as a target in obesity-related inflammatory state. Endocr Metab Immune Disord Drug Targets. 2015;15:145–50.
Kaplan JL, Marshall MA, McSkimming CC, Harmon DB, Garmey JC, Oldham SN, et al. Adipocyte progenitor cells initiate monocyte chemoattractant protein-1-mediated macrophage accumulation in visceral adipose tissue. Mol Metab. 2015;4:779–94.
Kanda H, Tateya S, Tamori Y, Kotani K, Hiasa K, Kitazawa R, et al. MCP-1 contributes to macrophage infiltration into adipose tissue, insulin resistance, and hepatic steatosis in obesity. J Clin Investig. 2006;116:1494–505.
Virgin HW, Levine B. Autophagy genes in immunity. Nat Immunol. 2009;10:461–70.
Maixner N, Kovsan J, Harman-Boehm I, Blüher M, Bashan N, Rudich A. Autophagy in adipose tissue. Obes Facts. 2012;5:710–21.
JK and NK designed and planned the study. JK, SP, MN, PB carried out the experimental work, biochemical and statistical analysis. JK, NK, MN performed interpretation and discussion of results. JK, MB and MN drafted and revised the manuscript. All authors read and approved the final manuscript.
We would like to thank Daniela Kern, Eva Böge and Jenny Schuster for technical assistance.
The authors declare that they have no competing interests.
Availability of data and materials
All data from experiments are available.
Consent for publication
Ethics approval and consent to participate
Experiments followed the international guidelines of animal care and the study protocols were approved by the Landesdirektion Leipzig, the local authority for animal care (T01/13 and T08/16).
This work was supported by grant of the IFB Adiposity Diseases, Federal Ministry of Education and Research (BMBF), Germany (K7-64 to JK and 01E01501 to NK) and a grant of Deutsche Forschungsgemeinschaft the SFB 1052/2 “Obesity mechanisms” (Project B01 to MB and B04 to NK) as well as DZD (82DZD00601).
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Expression of Mcp-1 mRNA in visceral and subcutaneous fat depots of WOKW and LEW.1W rats. Mcp-1 mRNA expression was increased in the visceral and subcutaneous fat depots of obese WOKW rats with MetS as compared to lean LEW.1W healthy control rats. Data from four individuals per group are presented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, according to the one-way analysis of variance together with the Newman–Keuls test.
About this article
Cite this article
Kosacka, J., Nowicki, M., Paeschke, S. et al. Up-regulated autophagy: as a protective factor in adipose tissue of WOKW rats with metabolic syndrome. Diabetol Metab Syndr 10, 13 (2018). https://doi.org/10.1186/s13098-018-0317-6