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Fig. 4 | Diabetology & Metabolic Syndrome

Fig. 4

From: Up-regulated autophagy: as a protective factor in adipose tissue of WOKW rats with metabolic syndrome

Fig. 4

Expression of proinflammatory markers: MCP1 and TNFα and protective adiponectin in cultivated adipocytes of WOKW and LEW.1W rats. Representative Western blots (a) and corresponding densitometrical analyses of adiponectin, MCP1 and TNFα (bd). a, b Western blot analysis revealed the decreased expression of adiponectin in visceral adipocytes of obese WOKW rats with MetS as compared to lean LEW.1W healthy rats. The adiponectin expression in subcutaneous adipocytes of both strains was slightly detected. The inhibition of PI3K-dependent autophagy pathway resulted in significantly upregulation of adiponectin levels, pronounced in adipocytes of WOKW rats. a, c, d Increased expression of MCP1 and TNFα protein was predominantly detected in visceral adipocytes of WOKW rats as compared to LEW.1W rats. These proteins could be significantly downregulated through autophagy inhibition (48 h) in both strains. Data from n = 6 are presented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, according to the one-way analysis of variance together with the Newman–Keuls test. GAPDH was used as normalization control

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