Fig. 1

Inhibition of PI3K-dependent autophagy activation using an LY294002 inhibitor (50 µM) in cultivated adipocytes of WOKW and LEW.1W rats. Representative Western blots of PI3K p85, pPI3K p85, Akt and pAkt in cultured adipocytes, untreated (−) or treated (+) with LY294002 (50 µM). Note that inhibition of the PI3K signaling pathway for 45 min resulted in significant inhibition of phospho-PI3K and phospho-Akt expression in visceral and subcutaneous adipocytes of WOKW and LEW.1W rats