High fat diet enhances cardiac abnormalities in SHR rats: Protective role of heme oxygenase-adiponectin axis
- Jian Cao†1,
- Komal Sodhi†2,
- Nitin Puri2,
- Sumit R Monu2,
- Rita Rezzani3 and
- Nader G Abraham2Email author
© Cao et al; licensee BioMed Central Ltd. 2011
Received: 11 October 2011
Accepted: 23 December 2011
Published: 23 December 2011
High dietary fat intake is a major risk factor for development of cardiovascular and metabolic dysfunction including obesity, cardiomyopathy and hypertension.
The present study was designed to examine effect of high fat (HF) diet on cardio-vascular structure and function in spontaneously hypertensive rats (SHR), fed HF diet for 15 weeks, a phenotype designed to mimic metabolic syndrome.
Development of metabolic syndrome like phenotype was confirmed using parameters, including body weight, total cholesterol and blood pressure levels. High fat diet impaired vascular relaxation by acetylcholine and exacerbated cardiac dysfunction in SHRs as evidenced by lower left ventricular function, and higher coronary resistance (CR) as compared to controls (p < 0.05). The histological examination revealed significant myocardial and peri-vascular fibrosis in hearts from SHRs on HF diet. This cardiac dysfunction was associated with increased levels of inflammatory cytokines, COX-2, NOX-2, TxB2 expression and increase in superoxide (O2-) levels in SHR fed a HF diet (p < 0.05). HO-1 induction via cobalt-protoporphyrin (CoPP,3 mg/kg), in HF fed rats, not only improved cardiac performance parameters, but also prevented myocardial and perivascular fibrosis. These effects of CoPP were accompanied by enhanced levels of cardiac adiponectin levels, pAMPK, peNOS and iNOS expression; otherwise significantly attenuated (p < 0.05) in HF fed SHRs. Prevention of such beneficial effects of CoPP by the concurrent administration of the HO inhibitor stannic mesoporphyrin (SnMP) corroborates the role of HO system in mediating such effects.
In conclusion, this novel study demonstrates that up-regulation of HO-1 improves cardiac and vascular dysfunction by blunting oxidative stress, COX-2 levels and increasing adiponectin levels in hypertensive rats on HF diet.
Keywordsheme oxygenase adiponectin high fat diet COX-2 oxidative stress
Obesity and hypertension are two major risk factors that lead to increased incidence of cardiac diseases including coronary artery disease, heart failure and cardiomyopathy [1–3]. Blood pressure, which strongly correlates with body mass index, is one of the most important determinants of cardiovascular function . In addition, obesity also leads to abnormal cardiac function through mechanisms that are independent of hypertension [5, 6]. Metabolic syndrome is a clinico-pathological condition which entails superimposition of these abnormalities and is characterized by systemic inflammation and oxidative stress [3, 7] A combination of these risk factors leads to disruption of metabolic homeostasis and may further contribute towards progressive cardiovascular dysfunction.
The heme-HO system, comprising of HO-1 (inducible) and HO-2 (constitutive) isoforms, is one of the key defense mechanisms against oxidative stress . This effect of HO system is attributable, in large part, to the antioxidant and anti-apoptotic properties of the heme degradation products, bilirubin/biliverdin and carbon monoxide (CO) . Previous studies have shown that upregulation of HO-1 exerts a cardio protective effect in hypertensive rats [10–14] by reducing myocardial hypertrophy, oxidative stress and inflammation. Over expression of HO-1 is also known to cause adipose tissue remodeling by increasing adiponectin in obese and non-obese diabetic rats and mice [15–18] along with obesity associated suppression of inflammatory cytokines. Adiponectin is an adipose tissue-specific protein that has been shown to have antiatherogenic, antihypertensive and insulin-sensitizing properties [19–21]. An inverse relationship exists between plasma adiponectin levels and systolic blood pressure as well as vascular dysfunction in obese subjects and animals [19, 22]. HO-1 functions as a stress response/chaperone protein and increases adiponectin levels which may cause activation of AMPK-AKT signaling [23–25], which contributes to improved NO bioavailability, vascular function, glucose transport and fatty acid oxidation [26, 27]. Thus, alterations in the heme-HO system not only influence vascular function but also modulate metabolic and cardiovascular processes which, in turn, are dependent upon activation of adiponectin/AMPK pathways.
The beneficial role of HO enzyme system in animal models of obesity and hypertension are clearly defined but paucity of evidence exists regarding similar effects in co-morbid conditions such as hypertension and obesity. In light of this evidence, the aim of this novel study was to explore the potential effect of HO-1 induction in spontaneously hypertensive rats (SHR) fed a high fat diet, a phenotype designed to mimic metabolic syndrome. We tested our hypothesis by using a well-described high fat regimen  that does not cause atherosclerotic lesion formation in mice , to address the effects of a known HO-1 inducer, cobalt protoporphyrin (CoPP). To verify that the effects of CoPP were due to an increase in HO-activity, we also treated a group of SHR concurrently with stannous mesoporphyrin (SnMP) to inhibit HO activity. Our results show that obesity exacerbates myocardial and vascular damage in SHRs, and HO-1 induction improves heart function in parallel with increased adiponectin levels and reduced expression of myocardial pro-inflammatory enzymes such as COX-2 and iNOS. Thus, HO-1 appears to play a critical role in the cellular defense against obesity-induced cardiovascular dysfunction in a hypertensive animal model fed a high fat diet. These findings may have important clinical implications in the management of patients with metabolic syndrome.
All animal studies were approved by the New York Medical College Animal Care and Use Committee in accordance with the National Institutes of Health Guidelines for Care and Use of Laboratory Animals. Fifty-eight seven-week-old male SHRs were purchased from Charles River Laboratories and were divided into four groups: A) SHR control, B) SHR-fat, C) SHR-fat and CoPP treatment, D) SHR-fat and CoPP and SnMP treatment. SHR rats were fed ad libitum either with a normal diet (group A) containing 11% fat, 62% carbohydrate, and 27.0% protein total, 12.6 KJ/g or a high fat diet (groups B, C, D) containing 58% fat from lard, 25.6% carbohydrate, and 16.4% protein yielding 23.4 KJ/g (Bio-SERV, Frenchtown, NJ) for 15 weeks [28, 30]. The diet used is distinct from the so-called "Western" or "atherosclerotic" diet which contains, in addition to high fat, cholesterol and bile acids. While the high fat diet used in the present study results in obesity, it does not cause atherosclerotic lesion formation in mice . After 4 weeks of high fat diet, cobalt protoporphyrin (CoPP), an inducer of HO-1, was administered intraperitoneally once a week (3 mg/kg) for 11 weeks to SHR rats maintained on a high fat diet. Some of the SHR treated with CoPP were concurrently treated with tin mesoporphyrin IX dichloride (SnMP), to inhibit HO activity, which was administered intraperitoneally three times a week (20 mg/kg)  to ascertain that any effects of CoPP treatment were related to increased HO activity. The untreated SHR rats maintained on the high fat diet were administered the vehicle for CoPP and SnMP once a week and 3 times a week respectively (0.1 mM sodium citrate buffer pH 7.8) for 11 weeks.
Rats were weighed every 7 days and systolic blood pressure was determined weekly by the tail-cuff method.
After a 6-hour fast, rats were anesthetized with sodium pentobarbital (65 mg/kg, i.p.) and blood was obtained from a tail vein for glucose measurement using a glucometer (Lifescan Inc., Miligitas, CA). Blood samples were then collected and stored as previously described .
Isolated Heart Preparation
Three days after the last CoPP (or vehicle) injection, rats were anaesthetized with pentobarbital, i.p., and heparinized via the left femoral vein (250 units/kg). The heart was rapidly excised, placed in cold perfusion medium and weighed. The isolated hearts were attached to the Langerdorff apparatus and retrogradely perfused (at 37°C) using constant perfusion pressure of 80 cm H2O, then perfusion pressure was decreased to 20 mmHg for 30 min, and then pressure was increased back to 80 mmHg for the remaining 30 min (reperfusion) . The perfusion medium consisted of oxygenated Krebs-Henseleit buffer [31, 32]. For measurement of ventricular systolic and end diastolic pressure (EDP), latex balloons were inserted into the left ventricle of the heart through the mitral valve and connected to a Harvard pressure transducer. In each experiment EDP was set at 10 mmHg and kept stable during the first 10 minutes of perfusion. Coronary perfusion pressure (CPP) was monitored by a second pressure transducer connected to the aortic cannula. Left ventricular developed pressure (LVDevP), EDP, dP/dTmax and dP/dTmin were all derived or calculated from the continuous monitoring of the LV pressure signal. In all experiments, coronary flow was continuously monitored by collecting the cardiac effluent. Coronary resistance (CR) was defined as input pressure divided by coronary flow per gram of myocardial tissue (mmHgxminxg/mL). At the end of each experiment, hearts were collected, half were used for histology examination and half of them were rapidly frozen in liquid nitrogen and stored at -80°C.
Assessment of Myocyte Cross-Sectional Area, Myocardial Fibrosis and Collagen in Myocardial Tissue
Hearts were fixed in 10% buffered formalin, and embedded in paraffin wax and sectioned to 5 μm. For measurement of the cross-sectional area, 100 cells (per animal) from the left ventricular wall were randomly chosen and analyzed in hematoxylin staining. The myocyte cross-sectional area and myocardial fibrosis were quantitatively analyzed with Image Pro-Plus 4.5.1 software in digitalized microscopic images. Myocardial fibrosis in the tissue sections was quantitatively analyzed by morphometry in 2 ways: (1) on the perivascular fibrosis, and (2) on myocardial tissue (total fibrosis index). The collagen in myocardial tissue was visualized by Sirius Red staining under polarization microscopy and then quantified.
Assessment of Vascular Reactivity
The aorta was removed, cleaned of fat and loose connective tissue, placed in cold Krebs-bicarbonate solution, and sectioned into 3-mm-long rings. Vasorelaxation responses of phenylephrine-constricted arteries to cumulative increments in acetylcholine (10-9 to 10-4 mol/L) were examined in the presence of indomethacin (10 μmol/L) as described .
Western Blot Analysis of Cardiac Tissue for protein expression
At the time of sacrifice, hearts were harvested, and stored at -140°C. Frozen hearts were pulverized under liquid nitrogen and placed in a homogenization buffer prior to immunoblotting with antibodies against HO-1, and HO-2 (Stressgen Biotechnologies Corp., Victoria, BC), COX-2, TX synthase, NOX-2, AKT, AMPK, pAMPK(Thr172), pAKT and adiponectin (Cell Signaling Technology, Inc., Beverly, MA) and eNOS, peNOS(serine 1177), and iNOS (Santa Cruz Biotechnology, Santa Cruz, CA). Immunoblotting was performed in cardiac tissue as previously described [15, 33].
Measurement of HO activity
HO activity in heart tissue was assayed as described previously  using a technique in which bilirubin, the end product of heme degradation, was extracted with chloroform, and its concentration was determined spectrophotometrically (dual UV-visible beam spectrophotometer Lambda 25; PerkinElmer Life and Analytical Sciences, Waltham, MA) using the difference in absorbance at a wavelength from 460 to 530 nm, with an extinction coefficient of 40 mM-1 cm-1.
Measurements of O2- production and total cholesterol levels
Total cholesterol was measured in serum using a cholesterol Quantification Kit (Biovision, Mountainview, CA) according to the manufacturer's instructions. For the detection of O2-, homogenized hearts were placed in plastic scintillation vials containing 5 μmol/l lucigenin in a final volume of 1 ml of air-equilibrated Krebs solution as described previously .
Plasma Adiponectin and inflammatory cytokines Measurements
The high molecular weight (HMW) HMW form of adiponectin, IL-6, TNF-α and TXB2 levels were determined using an ELISA assay (Pierce Biotechnology, Inc., Woburn, MA) as described previously .
The data are presented as mean ± standard error (SEM) where n = 6/group for the results. For comparison between treatment groups, the Null hypothesis was tested by a single factor analysis of variance (ANOVA) for multiple groups or unpaired t- test for two groups. Statistical significance (p < 0.05) between the experimental groups was determined by the Fisher method of analysis for multiple comparisons.
Effect of a high-fat diet on body weight and metabolic response
Systolic blood pressure was increased over the 15-week period in SHR rats (Figure 1B; n = 6/group). The systolic blood pressure was 175 ± 11 mmHg in the SHR control and was significantly increased in the rats fed a high fat diet, 211 ± 9 mmHg (p < 0.05). The elevation in systolic pressure was attenuated by CoPP treatment in SHR fed a high fat diet whereas SnMP treatment nullified the antihypertensive effect of CoPP in SHR fed a high-fat diet (Figure 1B). The mean blood glucose level in the SHR rats maintained on a normal diet was 128 ± 4 mg/dl, and was increased to 173 ± 14 mg/dl by a high fat diet (p < 0.05; n = 6/group) (data not shown). This increase in blood glucose levels was significantly attenuated by CoPP treatment in SHR rats fed a high fat diet (137 ± 4.5 mg/dl) and this effect was reversed by treatment with SnMP (180 ± 7.8 mg/dl) (data not shown).
Plasma cholesterol levels remained elevated in SHRs fed a high-fat diet as compared to their controls. Plasma cholesterol levels were 0.55 ± 0.11 in SHRs fed a normal diet for 15 weeks, and levels were increased to 1.25 ± 0.15 mg/dL by 15 weeks on the high-fat diet (P < 0.05) (Figure 1C). CoPP treatment prevented the increase in cholesterol levels in SHR while concomitant treatment with SnMP blocked the effect of CoPP.
Effect of high fat diet on cardiac parameters
Effect of high fat diet on CR and cardiac function during ischemia/reperfusion
Effect of high fat diet on Vascular Reactivity and superoxide levels
Effect of high fat diet on plasma adiponectin, inflammatory cytokines and TxB2 Levels
Effect of high fat diet on Cardiac COX-2, TxA2 and NOX-2 Levels
Effect of high fat diet on cardiac HO-1
Effect of high fat diet on Cardiac adiponectin, pAMPK and pAKT Expression
Effect of high fat diet on Cardiac eNOS, peNOS and iNOS Levels
Compared to animals fed a normal diet, SHR animals fed a high fat diet exhibited lower levels of eNOS and peNOS protein (p < 0.05) (Figure 8) CoPP administration produced an enhanced expression of eNOS and peNOS protein (p < 0.05 compared to untreated animals) in SHRs fed a high fat diet (Figure 8). In contrast, SnMP administration resulted in eNOS and peNOS protein in SHRs fed a high fat diet (Figure 8). Hearts isolated from SHRs fed a high fat diet showed a significant increase in iNOS expression compared to animals fed a normal diet (p < 0.05, respectively) (Figures 8). Treatment with CoPP resulted in a decrease in iNOS in SHRs fed a high fat diet (p < 0.0, Figure 8). In contrast, SnMP did not prevent the increase of iNOS expression in SHRs fed a high fat diet (Figures 8).
The results of the present study demonstrate that SHR fed a high fat diet develop patho-physiological abnormalities similar to that observed in metabolic syndrome. This phenotype is characterized by increased levels of body weight, blood cholesterol and blood pressure along with an accelerated decline in cardiac function when compared to SHR maintained on a normal diet. We, also, demonstrated that cardiac HO-1 induction, accompanied by increased plasma and tissue adiponectin levels, resulted in the improvement of cardiovascular function as manifested by a decrease in blood pressure, coronary resistance (CR), myocardial fibrosis; and increase in left ventricular function and vascular relaxation, as compared to control. The upregulation of HO-1 was associated with a concomitant decrease in the levels of O2-, COX-2 and iNOS, markers of oxidative stress. Furthermore, there was a decrease in cardiac remodeling, and an increase in the expression of cardiac pAKT, pAMPK and peNOS via induction of HO-1-adiponectin axis. To the best of our knowledge, this is the first report showing a protective effect of HO-adiponectin axis in a co-morbid condition where a pre-existing cardio-vascular pathology is further aggravated by addition of a HF diet.
High fat intake increased body weight, serum cholesterol and blood pressure in SHR and these changes in metabolic indices were associated with cardiovascular dysfunction in these animals. Previous studies have shown that HO-1 induction decreases obesity, reduces levels of visceral and subcutaneous fat and normalizes the metabolic profile in obese rats and mice [15, 17, 36, 37]. Also HO-1 overexpression is known to improve cardiovascular dysfunction in hypertensive rats [7, 11]. In contrast, in the current study we induced a metabolic syndrome-like phenotype in hypertensive animals. SHR demonstrate chronic hypertension, oxidative stress and cardiac damage . All of these parameters were worsened by the addition of high fat diet, strengthening our hypothesis that obesity and the associated metabolic abnormalities accelerate pathological pre-existing cardiovascular changes. Reversal of these patho-physiological abnormalities by HO-1-adiponectin induction corroborates the protective effects of the heme-oxygenase system in such a setting.
Metabolic syndrome-mediated increases in oxidative stress contribute to cardiovascular dysfunction via endothelial cell sloughing and beta cell apoptosis . Sustained increases in O2- levels and cytokines, including TNF-α and its receptor, lead to monocyte phenotype transition, myocytic apoptosis, and activation of matrix metalloproteinase. This, in turn, modifies the interstitial matrix, augmenting further ventricular remodeling [40, 41]. COX-2 is considered a pro-inflammatory enzyme as free radicals and prostaglandins (PGs) are produced during its catalytic cycle . It has been shown in our previous reports that upregulation of HO-1 decreases vasoconstrictors, such as cyclooxygenase (COX-2), PGs and thromboxane syntheses (TxA2) levels [8, 42] by regulating the cellular heme levels and ROS. The heme-HO system is a stress response system (reviewed in  that undergoes activation under conditions of increased oxidative stress such as those presented here. Induction of HO-1 resulted in decreased cardiac levels of superoxide and NOX-2 expression which may be due to a decrease in the levels of NADPH oxidase , a heme-dependent protein, and/or an increase in the levels of superoxide dismutase EC-SOD . Also in the present study, increased cardiac iNOS expression and impaired vascular relaxation in rats fed a high-fat diet was reversed by HO-1 induction which may involve the interplay of one of the various mechanisms including, CO generation, HO-1-induced increase in eNOS expression and increased NO bioavailability due to an increase in cellular antioxidants [37, 45–47].
In the present study, a decrease in coronary vascular reactivity manifested by coronary resistance, myocardial fibrosis and cardiac function was found in SHRs fed a high fat diet. The increase in expression of HO-1/adiponectin reverses these deleterious effects with a resultant improvement in energy metabolism and an amelioration of the damaged endothelial and cardiac function seen in SHRs fed a high fat diet. We studied coronary microvascular reactivity and hemodynamics in the isolated, empty, beating heart of SHRs fed a high fat diet. This was prevented in CoPP-treated animals by SnMP suggesting the seminal role of increased HO activity in instigating the changes attributable to increased HO-I expression. This finding highlights the role of the HO system in the preservation of microvascular and cardiac function.
Apart from effects on heme degradation products, HO1 up-regulation was associated with increased cardiac and plasma levels of adiponectin. This causality between HO activity and adiponectin release was strengthened by the inhibitory effects of SnMP on both HO activity and adiponectin levels. It has been recently shown that the beneficial effects of heme- HO system in established cardiovascular-metabolic disorders is mediated, at least in part, via its effect on adiponectin-dependent pathways [15, 48, 49]. Results presented in the current study support and advance our hypothesis that, in addition to its antioxidant properties, the heme-oxygenase system enhances the adiponectin axis which, in turn, modulates multiple physiological processes and may contribute towards HO-mediated attenuation of cardiac dysfunction [17, 18, 50].
The HO-1-mediated increase in adiponectin was associated with an increase in cardiac pAMPK-pAKT signaling and cross-talk between AMPK and AKT levels appear to correlate with HO-1 and adiponectin levels [16, 18, 25, 51]. This is of particular importance in the setting of myocardial ischemia of SHR rats fed a high fat diet due to the very-high-energy demands and low-energy reserves of the heart. Amplifying signaling through AMPK by HO-1 induction during early reperfusion is beneficial to the injured myocardium due to the ability of AMPK to promote ATP generation [52, 53] and to attenuate cardiomyocyte apoptosis . An increase in AMPK-AKT signaling is considered an important metabolic response that is necessary for the attenuation of ROS-mediated cardiac and endothelial dysfunction  and both pAMPK and pAKT use eNOS as a substrate and enhance the levels of peNOS [8, 56, 57]. The results of this study support this link as induction of HO-1-adiponectin axis, also, increased peNOS expression in the heart of SHR. The seminal role of increased HO-1 expression and HO activity in cardiac protection is further strengthened by the results obtained when SnMP was concurrently administered with CoPP; the inhibition of HO activity prevented the beneficial effects of HO-1 induction in obese SHR with regard to blood pressure, adiponectin, pAKT and pAMPK. In summary, these observations support the beneficially role of pharmaco-genetic interventions targeted towards HO-1-adiponectin axis in patients with metabolic syndrome. Such patients often exhibit chronic energy imbalance along with a wide array of cardiovascular abnormalities amenable to aggravation by confounding factors such as diet induced obesity. Restoration of metabolic homeostasis by activation oh HO-1-adiponectin axis could not only improve the energy profile but also attenuate associated cardiovascular patho-physiological alterations observed in the patients with metabolic syndrome.
In conclusion, the results of the present study demonstrate that upregulation of HO-1 in association with increased levels of adiponectin prevents vascular and cardiac dysfunction in SHRs fed a high fat diet, a phenotype designed to mimic metabolic syndrome. The pharmacological enhancement of HO-1 expression, resulting in a phenotype resistant to injurious stimuli, permits the heart to initiate a crucial and immediate defense against the events associated with the metabolic syndrome, thereby preventing the continued deterioration in cardiac function associated with this disease.
All authors had full access to the data and take responsibility for its integrity. All authors have read and agree with the manuscript as written. We also thank Jennifer Brown for her outstanding editorial assistance in the preparation of the manuscript.
This work was supported by NIH grants DK068134, HL55601 and HL34300 (NGA).
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