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The effect of curcumin supplementation on anthropometric indices, insulin resistance and oxidative stress in patients with type 2 diabetes: a randomized, double-blind clinical trial
Diabetology & Metabolic Syndrome volume 11, Article number: 41 (2019)
Diabetes mellitus is a common metabolic disorders in human and affect a lot of people around the world. Curcumin is a component of turmeric and in many studies therapeutic effects such as anti-hypertensive, anti-hyperlipidemia, anti-hyperglycemia for this substance are shown.
The aim of this study was to investigate the effect of curcumin supplementation on anthropometric indices glycemic control and oxidative stress in overweight patients with type 2 diabetes.
Materials and methods
In this randomized, double-blind, placebo-controlled trial, 53 participants with type 2 diabetes were divided randomly into the experimental and control groups to receive either 1500 mg curcumin or placebo capsule three times in a day for 10 weeks.
Supplementation with curcumin in type 2 diabetes compare to placebo causes a significant changes in mean weight (− 0.64 ± 0.22 vs. 0.19 ± 0.37 p < 0.05), body mass index (BMI) (0.3 ± 0.03 vs. 0.1 ± 0 p < 0.05), waist circumference (WC) (− 1.2 ± 0.4 vs. − 0.43 ± 0.11 p < 0.05) and fasting blood sugar (FBS) (− 7 ± 2 vs. 3 ± 0.2 p < 0.05) but did not show any difference for hemoglobin A1c (HbA1c), insulin, malondialdehyde (MDA), total antioxidant capacity (TAC), Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) and pancreatic B cell function (HOMA-B) at end of study.
This study indicated that daily administration of 1500 mg curcumin has positive effects in reducing fasting blood glucose and weight in patients with type 2 diabetes.
Trial registration NCT02529982. Registered 19 August 2015, http://www.clinicaltrial.gov
Diabetes mellitus is the most common and important metabolic disorders in human. According to World Health Organization (WHO) report in 2016, 422 million people in world are diagnosed with diabetes, although the prevalence of this disorder is on the rise, especially in Asian countries . Also recent researches showed that obesity affects more than 600 million people in worldwide. Obesity and overweight are two major risk factors for diabetes and its complications. Investigations show that almost 90% of diabetic patients are overweight. Obesity could affect the metabolism in the body by insulin resistance and reducing insulin secretion .
Curcumin is the most important component of turmeric (curcuma longa) and has a yellow color. Curcumin commonly used as a food additive in Asian countries . Also this substance has a Therapeutic characteristics and had role in traditional medicine as medicinal herb in india and china . Curcumin can cause a significant reduction on total cholesterol, blood pressure, aggregation platelet, myocardial infraction and prevention from thrombosis, rheumatoid arthritis [5, 6].
In some clinical trials Desirable effects of curcumin has shown to be a therapeutic agent for treatment of diabetes and complications of High blood glucose. Curcumin as an antioxidant and anti-inflammatory agent has different effects on diabetes through reducing death of beta cells, improving its function and prevent insulin resistance in rodent models [7,8,9,10,11]. Also curcumin thanks to anti-inflammatory effect has positive impression on weight loss. Give that obesity is associated with chronic inflammation, curcumin as an anti-inflammatory phytochemical has been studies .
Most of studies conducted for investigated effects of curcumin is on animal model [9, 13,14,15,16,17,18,19,20] and studies in human model is rare and many effects of this substance in humans is undiscovered. Therefore the aim of this study was to investigate the effects of curcumin supplementation on glycemic control, oxidative stress and anthropometric indices in patients with type 2 diabetes.
Method and materials
All of the participants in this study were adult patients who had come to the health centers of district 2 of Tehran/Iran. Patients with noninsulin dependent diabetic, aged between 40 and 70 years old and BMI 18.5–35 kg/m2 with a diagnose of 1 to 10 years participant in this study. Patients were excluded from the trail if they were suffering from inflammatory, hepatic and renal disease, and receiving multivitamin and mineral supplements or herbal medicines during last 3 months. They were informed about aims of study and written informed consent was obtained from all of them. Patients were educated not to change their usual dietary habits, physical activity, lifestyle and drugs during the intervention period otherwise, they were excluded from the study. This randomized, double-blinded, placebo-controlled trial was conducted at National Nutrition And Food Technology Research Institute of Shahid Beheshti University. This clinical trial has been registered in the clinical Trail.gov with the following identification: NCT02529982.
This study was a randomized, double blind, placebo-controlled trial. Patients were matched for gender and then randomly allocated to the curcumin group (n = 25) meals or placebo group (n = 28) respectively for consume three capsules, of 500 mg of curcumin and placebo after each main mail in three doses by block randomization for 10 weeks. Curcumin and placebo capsules were prepared by Arjuna National Extract Ltd company of India. Based on studies receiving 7 gr curcumin or placebo do not increase blood glucose in patients with diabetes . Each curcumin capsules had curcuminoid content of 440 mg (347 mg curcumin, 84 mg desmethoxycurcumin and 9 mg bis-desmethoxycurcumin) and 38 mg of turmeric oil. Placebo capsule had the same shape and color of curcumin capsule and contain 444 mg of cooked rice flour. Random allocation of patients were performed by a third person. Throughout the course of the intervention researchers and participants were not aware about allocation.
In order to control patients for taking supplements and placebo they were followed-up by phone every 15 days and Supplement and placebo were given to the patients for a period up to the middle of the study, and patients were asked to bring the bottle of capsules in their next visit for assessing their compliance and if anyone used less than %90 of capsules he would be excluded.
Measurement of anthropometric parameters and blood pressure
Anthropometric measurements, including weight, height, waist and hip circumferences were measured at baseline and at the end of the study. The weight of each patient was measured without shoes and light clothing by using seca scale with the precision of 100 gr and height was also measured by using a stadiometer without shoes with precision of 0.5 cm. To measure waist circumference tape measure was placed about halfway between the bottom of the lowest rib and the top of hip bones, and to measure hip circumference tape measure was placed in widest part of hip, each measurements was repeated twice. Body mass index (BMI) was calculated as weight (kg) divided by square of height (m). Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured twice in a sitting position on the right arm after 15 min of rest and the mean of the two measurements was considered as the subject’s blood pressure.
Assessment of dietary and physical activity
Dietary intake of each participant in this study assessment at baseline, at the 5th week and end of study by 3 day records, patients were enlighten about portion size and how to record their dietary intake and then all records converted to grams and analyzed by using Nutritionist IV software (N Squared Computing, San Bruno, CA, USA). To assess physical activity during intervention, valid physical activity questionnaire was completed for all participates at the begging and the end of study .
Measurement of biochemical parameters
A sample of 10 ml blood was taken from each patients after 12–14-h fasting at baseline and the end of study. Serum concentration of glucose, total antioxidant capacity (TAC), and Malondialdehyde (MDA) were determined by using colorimetric method (Zellbio, Germany). Insulin level was measured by ELISA method, and Hemoglobin A1c (HbA1c) level was measured by chromatography method (Zellbio, Germany). Levels of Change in pancreatic B-cell function (HOMA-B) and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) were calculated by formulas respectively, HOMA-B = 20 × Insulin(µU/ml)/glucose(mg/dl) − 3.5 and HOMA-IR = (glucose(mg/dl) × Insulin(µU/ml))/22.5 .
Data were analyzed by using SPSS 22 software. For comparison of quality variables Chi square tests were used between the two groups. Normality of quantity variables were assessed by Kolmogorov–Smirnov test. independent t-tests and paired t-tests were used to compare parameters between and within the groups, respectively. The results were displayed as mean ± SD, and a P-value < 0.05 was considered significant. The minimum number of patients estimated for each group was 22 at a power (1−β) 80% and α = 0.05 for this parallel study, for detect a difference of 1.7 mg/dl in fasting blood sugar concentration with a standard deviation of 1.8 mg/dl .
Based on the flow chart of the trial 44 participates completed the study. 44 patients were randomly divided in curcumin group (n = 21) and placebo group (n = 23) (Fig. 1). None of the patients did not reported any serious side effect. There were no significant differences in baseline characteristics include sex distribution, smoking, mean age, duration of diabetes and drugs between the two groups at baseline and the end of week 10 (Table 1).
Statistical analysis could not show any significant differences between two groups with regard to physical activity, mean energy, the dietary intake of carbohydrate protein, fiber, total fat, SAFA, MUFA, PUFA, cholesterol, vitamin E and C and polyphenol intakes at baseline, week 5 and week 10 of the study (Tables 2 and 3).
At the end of the study mean body weight decreased significantly in curcumin group compared to the baseline (p = 0.01), and also analysis between two group had showed a significant reduce for mean body weight (p = 0.04). Also Mean BMI decreased significantly in curcumin group at the end of the study compared to the baseline (p = 0.03), however there was not any significant differences between two groups at the end of the stud. Mean hip circumference reduced significantly in curcumin group compared to baseline and placebo group (p = 0.05 and 0.01, respectively), but The mean waist circumference, systolic and diastolic blood pressure had no significant different between and within two groups (Table 4).
After 10 weeks intervention with curcumin mean serum concentration of fasting blood glucose decreased significantly in treatment group compared to placebo group at the end of the study (p = 0.02) but The mean serum concentrations of insulin, HbA1c, HOMA-IR, HOMA-B had no significant changes within each group or between groups during study and mean serum concentrations of MDA and TAC had no significant changes between two groups at the end of the study (Table 5).
In this randomized, double-blinded, placebo-controlled clinical trial, we indicated that curcumin supplementation has positive effect in reduction fasting blood glucose, body weight and hip circumference in patients with type 2 diabetes. Although there are some clinical trials that have described the effect of turmeric and its derivatives consumption on glycemic control, oxidative stress and anthropometric indices [3,4,5,6,7,8, 16, 17, 22,23,24,25], but most of these studies have been done on animal models and only a few of them have assessed the impact of curcumin on patients with diabetes and its complications. According to this study daily administration of 1500 mg curcumin could significantly reduce body weight and hip circumference in patients with type 2 diabetes. Also in one study it was showed, Supplementation with 500 mg curcumin for 4 weeks in obese children and adults showed a significant improve in serum adiponectin and lowered leptin levels that both of these factors play an important role in weight control . Also in another clinical trial have been reported that consumption 1200 mg turmeric for a period of 8 weeks could reducing BMI in patients with type 2 diabetes . There are different mechanisms for anti-obesity effects of curcumin, in its most important role declines the expression of genes involved in energy metabolism, lipid accumulation and reduction in intra cells fat . Diet therapy with curcumin in rodent model causes body weight loss and raise lean body mass. In fact, curcumin inhibits the replication of proteins from the pathway of adipogenesis as well as curcumin leads to increase resting metabolic rate (RMR). This component of turmeric have direct effects on lipid metabolism such as reducing TG synthesis and increasing in beta oxidation of free fatty acids with raising metabolism rate and releases some kind of cytokines which are effective in weight loss. On the other hand curcumin inhibits genes expression of inflammatory cytokines like 1, 2, 6, 8, 12 Interleukins and Tumor necrosis factor alpha (TNF-α) by inactivate Nuclear Factor Kappa Beta (NF-ƙB) .
This trial demonstrate that daily supplementation with 1500 mg curcumin for 10 weeks could decrease fasting blood glucose in patients with type 2 diabetes, but no significant changes observed in serum insulin level, insulin resistance and HBA1c. According to the study conducted on 2013, receiving 300 mg curcuminoid daily for 3 months could reduce fasting blood glucose, HbA1c and HOMA-IR in participants with type 2 diabetes . However daily supplementation with 2100 mg turmeric for 8 weeks had no significant effect on fasting blood glucose, serum insulin level and HbA1c . There are various mechanisms for anti-diabetic effect of curcumin. One of the most fundamental of these mechanisms is that improvement in beta-cells function through its anti-inflammatory and anti-oxidant properties [7,8,9, 28]. In addition curcumin reduces fasting blood glucose by increasing Peroxisome proliferator-activated receptor (PPAR) activity, inhibits hyperglycemic, increases Glycolysis, inhibits liver gluconeogenesis, stimulations secretion insulin from pancreas, provokes glucose uptake by increasing gene expression of GLUT4, GLUT2 and GLUT3, suppresses liver production through improvement AMP kinase activation and inhibits glucose 6 phosphate kinase [16, 26, 28]. On the other hand curcumin increases the expression of adiponectin genes and thus leads to raising insulin sensitivity .
This investigation showed that receive 500 mg curcumin capsule 3 times a day for period of 10 weeks had no significant effect on reducing serum malondialdehyde level and increasing total antioxidant capacity. Oxidative stress reflects an imbalance between production of free radicals and body antioxidant defenses, for example Hyperglycemic could increase production of free radicals and oxidative stress . Actually hyperglycemic through raising mitochondria free radicals production especially Reactive oxygen species (ROS) leads to oxidative stress in diabetes . Curcumin as an antioxidant agent could enhance the expression of antioxidant enzymes and thus increases total antioxidant capacity. Mechanisms that are described for the antioxidant effects of curcumin include, Inhibition of glycosylation of proteins and lipid peroxidation, increased glutathione peroxidase activity, increasing the expression of antioxidant enzymes and enhancing the free radical clearing capacity .
In a study that investigated the effect of curcumin on MDA level, curcumin caused significant reduction on MDA level. In other study oral curcumin in diabetic rodent leaded to increase all antioxidant enzymes activity probably due to improve access to glutathione reductase, increased expression of Superoxidase dismutase, catalase and glutathione reductase .
The present study had some limitation. First, it seems that 10 weeks administration of curcumin there is not enough time to influence the factors involved in this trial also It was not possible to continue the longer intervention period due to the loss of samples and second, lake of access to nonocurcumin, another form of curcumin that can have significantly therapeutic effect and nanorange formulation. Also there are some reports linking curcumin with iron deficiency anemia. So patients with iron deficiency anemia and using curcumin supplements should be tested for possible causality.
In conclusion our findings suggest that daily intake of 1500 mg curcumin powder reduces fasting blood glucose, weight and hip circumference in patient with type 2 diabetes, but had no effect on oxidative stress, serum insulin level, insulin resistance and HbA1c.
Availability of data and materials
Please contact authors for data request.
World Health Organization
body mass index
fasting blood sugar
total antioxidant capacity
Homeostatic Model Assessment for Insulin Resistance
pancreatic B-cell function
systolic blood pressure
diastolic blood pressure
resting metabolic rate
low density lipoprotein-cholesterol
tumor necrosis factor alpha
nuclear factor kappa beta
peroxisome proliferator-activated receptor
reactive oxygen species
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His study was supported by the grant from National Nutrition And Food Technology Research Institute, Shahid Beheshti University of Medical Sciences.
Ethics approval and consent to participate
The study protocol was approved by the ethics committee of the National Nutrition and Food Technology Research Institute of Shahid Beheshti University of Medical Sciences. Written informed consents were obtained from all participants.
Consent for publication
The authors declare that they have no competing interests.
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