The role of bone marrow derived-mesenchymal stem cells in attenuation of kidney function in rats with diabetic nephropathy
© Abdel Aziz et al.; licensee BioMed Central Ltd. 2014
Received: 24 September 2013
Accepted: 22 February 2014
Published: 9 March 2014
Stem cell therapy holds a great promise for the repair of injured tissues and organs, including the kidney. We studied the effect of mesenchymal stem cells (MSC) on experimental diabetic nephropathy (DN) in rats and the possible paracrine signals that mediate their action.
Materials and methods
Rats were divided into controls, DN rats, DN rats receiving MSCs. MSCs were given in a dose of (106cells) by intravenous injection. After 4 weeks, 24 h urinary albumin, serum urea and creatinine concentrations, transforming growth factor β (TGF β), tumor necrosis factor α (TNFα), B-cell lymphoma 2 (bcl2) and Bax gene expression and vascular endothelial growth factor (VEGF) were assessed. Histopathology staining was performed.
MSC therapy significantly improved 24 h urinary albumin, serum urea and creatinine concentrations, increased angiogenic growth factor VEGF, and anti-apoptotic protein bcl2 while decreased the pro-inflammatory TNF-α, fibrogenic growth factor TGF β, and pro-apoptotic protein Bax. The histopathology examination showed patchy areas of minimal necrosis and degeneration in renal tubules.
Diabetic nephropathy (DN) is the most common cause of end-stage renal disease in the world, and could account for disability and high mortality rate in patients with diabetes . DN is thought to result from interaction between metabolic and hemodynamic factors. The pathologic changes in DN include renal hypertrophy and extracellular matrix accumulation, which contribute to glomerular sclerosis, which leads to proteinuria and renal failure through the tubular interstitial fibrosis .
The basic underlying mechanisms of DN involve high-glucose (HG)-induced production of cytokines and growth factors, which promote leukocyte infiltration, renal cell proliferation, and matrix production [3, 4].
Stem cell therapy holds a great promise for the repair of injured tissues and organs, including the kidney. Stem cells are undifferentiated cells that undergo both self-renewal and differentiation into one or more cell types .
Among stem cells, mesenchymal stem cells (MSCs) have several advantages for therapeutic use such as ability to migrate to the sites of tissue injury, strong immunosuppressive effects [6, 7], and better safety after infusion of allogeneic MSCs [8, 9].
Previous studies have shown that MSCs are able to differentiate into several cell types, including cardiomyocytes, vascular endothelial cells, neurons, hepatocytes, epithelial cells, and adipocytes, making them a potentially important source for the treatment of debilitating human diseases. Such multipotent differentiation characteristics coupled to their capacity for self-renewal and capability for the regulation of immune responses, described MSCs as potentially new therapeutic agents for treatment of the complications of diabetes mellitus (DM) .
An increasing number of data has showed that the therapeutic effects of MSCs not only rely on their differentiation ability to repair damaged tissue, but also depend on their potency to modulate local environment, activate endogenous progenitor cells, and secrete various factors [11, 12].
The present study aims to detect the effect of mesenchymal stem cells on experimental DN in rats and to identify the paracrine signals that mediate MSCs action.
Materials & methods
Preparation of the animal model
The study was carried on 60 female albino rats, of an average weight 150–200 gm. Rats were bred and maintained in an air-conditioned animal house with specific pathogen free conditions, and were subjected to a 12:12-h daylight/darkness and allowed unlimited access to chow and water. All the ethical protocols for animal treatment were followed and supervised by the Animal Facilities, Faculty of Medicine, Cairo University. All animal experiments received approval from the Institutional Animal Care Committee.
Group 1 (Control group): 20 healthy female albino rats.
Group 2 (Diabetic Nephropathy group): 40 female albino rats in which type1 diabetes was induced by a single intra peritoneal injection of streptozotocin (STZ) [60 mg/kg body weight dissolved immediately before administration in freshly prepared 0.1 mol/L citrate buffer (pH 4.5)]. Diabetes was defined as a random blood glucose reading of >300 mg/dl in 3 continuous days after 72 hours of STZ injection . Diabetic nephropathy was confirmed after 12 weeks by measuring serum urea and creatinine in blood and also by histopathological changes.
Group 2 (Diabetic Nephropathy group) was further divided into two subgroups:
Group 2a: consisted of 20 DN rats which received IV PBS.
Group 2b: consisted of 20 DN rats which received MSCs (which were processed and cultured for 14 days), in a single dose of (106cells) per rat by intravenous injection in rat tail vein .
Four weeks after MSCs injection, each group was subjected to 24 hours urine collection for urinary albumin concentration measurement, blood sampling through the retro-orbital vein for blood glucose, urea and creatinine concentration estimation.
Quantitative analysis of TGF β, TNFα, bcl2 and Bax gene expression by real time PCR.
Histopathological examination of renal tissue by haematoxylin and eosin and by differential stains (massontrichrome MT and periodic acid shift PAS).
Immunohistochemical examination of VEGF expression.
Detection of the MSCs homing in kidney tissues after its labeling with PKH26 dye by fluorescent microscope to detect its red fluorescence.
Preparation of BM -derived mesenchymal stem cells from rats
Bone marrow was harvested by flushing the tibiae and femurs of 6-week-old male white albino rats with Dulbecco’s modified Eagle’s medium (DMEM, GIBCO/BRL) supplemented with 10% fetal bovine serum (GIBCO/BRL). Nucleated cells were isolated with a density gradient [Ficoll/Paque (Pharmacia)] and resuspended in complete culture medium supplemented with 1% penicillin–streptomycin (GIBCO/BRL). Cells were incubated at 37°C in 5% humidified CO2 for 12–14 days as primary culture or upon formation of large colonies. When large colonies developed (80–90% confluence), cultures were washed twice with phosphate buffer saline(PBS) and the cells were trypsinized with 0.25% trypsin in 1 mM EDTA (GIBCO/BRL) for 5 min at 37°C. After centrifugation, cells were resuspended in serum supplemented medium and incubated in 50 cm2 culture flask (Falcon). The resulting cultures were referred to as first-passage cultures . Cells were identified as being MSCs by their morphology, adherence, and their power to differentiate into osteocytes and chondrocytes. Differentiation into osteocytes was achieved by adding 1-1000 nM dexamethasone, 0.25 mM ascorbic acid, and 1-10 mM beta glycerophosphate to the medium. Differentiation of MSCs into osteoblasts was confirmed by morphological changes, Alzarin red staining of differentiated osteoblasts. Differentiation into chondrocyte was achieved by adding 500 ng/mL bone morphogenetic protein-2 (BMP-2;R&D Systems, USA) and 10 ng/ml transforming growth factor b3 (TGFb3) (Peprotech, London) for 3 weeks. In vitro differentiation into chondrocytes was confirmed by morphological changes, Alcian blue staining of differentiated chondrocytes.
Labeling of MSCs with PKH26
MSCs were labeled with PKH26 supplied by Sigma Company (Saint Louis, Missouri USA). Cells were centrifuged and washed twice in serum free medium. Cells were pelleted and suspended in dye solution. Cells were injected intravenously into rat tail vain. After one month, kidney tissues were examined with a fluorescence microscope to detect and trace the cells.
Real-time quantitative analyses for TNF-α, TGFβ, bcl2 and Bax gene expression
The oligonucleotide primers sequence of studied genes
Forward primer: 5′- GACCCTCACACTCAG ATC ATC TTC T -3′
Reverse primer: 5′- TTGTCTTTGAGATCCATGCCA TT -3′
Forward primer: 5′- AATGTCAGCTCAGGAACATCCA -3′
Reverse primer: 5′- GTTCCTGACACATGAACCCTTG -3′
Forward primer: 5′- GGAGGGCACTTCCTGAG -3′
Reverse primer: 5′- GCCTGGCATCACGACT -3′
Forward primer: 5′- CTGAGCTGACCTTGGAGC -3′
Reverse primer: 5′- GACTCCAGCCACAAAGATG -3′
Detection of VEGF by immunohistochemistry
Unstained positively charged slides were prepared from each paraffin block for immunostaining using monoclonal rabbit anti-human antibody (anti- VEGF, Lab vision, USA. Cat = RB-9072) and ultra-vision detection system (HRP/DAB, Lab vision, USA).
Positive immunoreactivity to VEGF shows a brown staining in renal endothelial cells of interstitial tissue.
Blood was collected from the retro-orbital vein into tubes containing fluoride. Plasma samples were separated by centrifugation at 3000 rpm for 10 min. Plasma glucose was measured by the glucose oxidase method using a commercially available kit (Diamond, Egypt).
Serum urea and creatinine levels were measured using the conventional colorimetric method using QuantiChrom TM assay kit based on the improved Jung and Jaffe methods, respectively (DIUR- 500 and DICT-500).
24 h Urinary albumin was assessed by using Albu well M Kit (Murine Microalbuminuria ELISA).
Analysis of kidney histopathology
Kidney samples were collected in PBS and fixed overnight in 40 g/L paraformaldehyde in PBS at 4°C. Serial 5-μm sections of the cortex and the medulla of the kidney were stained with hematoxylin and eosin (H&E).
Data were expressed as mean ± SD. Significant differences were determined by using ANOVA and post-hoc tests for multiple comparisons using SPSS version 12 computer Software. Results were considered significant at p < 0.05.
MSCs culture, identification & homing
MSCs improve the kidney function
Plasma glucose, serum urea, serum creatinine, urinary albumin and body weight in studied groups
Urea mean ± SD
Creatinine mean ± SD
Urinary albumin conc.
41.09 ± 2.00
0.20 ± 0.08
0.06 ± 0.02
86.74 ± 6.42
206 ± 6.20
84.11 ± 2.66#
0. 96 ± 0.05#
28.06 ± 1.92#
241.99 ± 43.60#
154 ± 9.60#
DN & MSC
55.79 ± 2.30#*
0.55 ± 0.05#*
6.11 ± 1.60#*
157.86 ± 23.68#*
204.1 ± 4.43*
Bcl2 and Bax gene expression
TGFβ and TNFα gene expression
Several different growth factors are known to be involved in the development of diabetic complications. Disturbed growth factor signaling adversely affects tissue function and influences the extracellular matrix (ECM). Changes in the amount and composition of ECM are observed in all complications of diabetes and have a central role in their progression .
Macrophages are key inflammatory cells mediating kidney inflammation in experimental and human diabetes. Activated macrophages elaborate a host of proinflammatory, profibrotic, and antiangiogenic factors. These macrophage-derived products include but are not limited to TNF-α, IL-1, IL-6, reactive oxygen species (ROS), plasminogen activator inhibitor-1 (PAI-1), matrix metalloproteinases, TGF β, platelet-derived growth factor (PDGF), angiotensin II, and endothelin . In experimental diabetic mice, macrophage accumulation and activation are associated with prolonged hyperglycaemia, glomerular immune complex deposition, increased chemokine production, and progressive fibrosis . In a human study, interstitial macrophage accumulation correlated strongly with serum creatinine, proteinuria, and interstitial fibrosis at the time of biopsy, and inversely with the renal function decline (slope of 1/serum creatinine) over the following 5 years . These human data support animal studies in suggesting a pathological role for macrophages in DN.
TNFα is a potent proinflammatory cytokine and an important mediator of inflammatory tissue damage. TNFα also has an immunoregulatory role . Reported actions of TNF-α on renal cells include the activation of second messenger systems, transcription factors, synthesis of cytokines, growth factors, receptors, cell adhesion molecules, enzymes involved in the synthesis of other inflammatory mediators, acute phase proteins, and MHC proteins . This variety of biologic activities results in diverse effects with a significant role in the development of renal damage in diabetes. TNF-α is cytotoxic to renal cells and able to induce direct renal injury . Also TNF-α causes induction of apoptosis and necrotic cell death [23, 24], alterations of intraglomerular blood flow and GFR as a result of the hemodynamic imbalance between vasoconstrictive and vasodilatory mediators  as well as alterations of endothelial permeability. TNF-α alters the distribution of adhesion receptors involved in cell–cell adhesion (i.e., vascular endothelial-cadherin-catenin complexes) and prevents the formation of F-actin stress fibers. This results in restructuring of the intercellular junction leading to loss of endothelial permeability . On the other hand, TNF-α directly induces reactive oxygen species (ROS) in diverse cells, including mesangial cells . In this study, there was a significant increase in TNFα gene expression in the diabetic nephropathy group compared to the control group. These results were in accordance with Sugimoto et al.,  who reported a significant rise in the expression of TNF-α in streptozotocin-induced diabetic rat glomeruli after diabetes induction. Advanced glycation end products (AGE), stimulate TNF-α synthesis by renal cells through binding to specific cell surface receptors (RAGE) of the immunoglobulin superfamily identified on several cell types, including renal cells [29, 30]. This interaction has been implicated in the development and progression of DN , induce a range of biologically important responses, including TNF-α synthesis and secretion [32, 33].
TGF-β is a fibrogenic growth factor involved in the pathogenesis of kidney damage and is locally produced in the kidney. It has been shown that TGF-β induces apoptosis of tubular epithelium cells and contributes to progressive renal tubular atrophy .
Bone marrow–derived stem cells contribute to cell turnover and repair in various tissue types, including the kidneys [35, 36]. MSCs are attractive candidates for renal repair, because nephrons are of mesenchymal origin and because stromal cells are of crucial importance for signaling, leading to differentiation of both nephrons and collecting ducts . In the present study, bone marrow derived mesenchymal stem cells were isolated from male rats, grown and characterized by their adhesiveness and fusiform shape and by detection of CD 29; one of the surface markers of rat mesenchymal stem cells, and were used to detect their possible anti-inflammatory, anti-apoptotic and vascular role in amelioration of renal function in experimental DN model. These cells were actually insinuating themselves into the renal tissue as detected by fluorescent microscope. Similar results have been reported by Morigi et al. , who. injected labeled human bone marrow MSCs with PKH 26 dye into mice with induced acute renal failure. The red fluorescence of the MSC was clearly detected in renal tissues.
The possible mechanism by which stem cells improve the kidney function either by fusion or transdifferentiation could not be answered in this study; however, both techniques showed that those cells were able to maintain high population all through the study following MSCs injection. Ling et al.,  showed 50% replacement of proximal tubular cells with donor cells. Also Rookmaaker et al.,  declared that bone-marrow-derived cells may home to injured glomerular endothelium, differentiate into endothelial cells, and participate in regeneration of the highly specialized glomerular microvasculature. In addition, they confirmed previous observations that bone-marrow-derived cells can replace injured mesangial cells . Togel et al.,  stated that infused MSCs were detected in the kidney only early after administration and were predominantly in the glomeruli.
Duffield et al.  stated that BMDC contribute in a regenerative cytokine environment in the resulting functional repair. Similarly, bone marrow–derived stem cells seemed to contribute to a relatively small numbers of cells (3 to 22%) to regenerating renal tubular  and glomerular cell populations ; that is, the majority of reparative cells were derived from intrinsic kidney cells. Regardless the cause, whether it’s MSC differentiation, fusion or merely cytokine induced renal improvement; in this study following MSC injection, there was an improvement of kidney functions, decrease in TNFα, TGFβ, Bax gene expression and increase in bcl2 gene expression and VEGF expression (by immunohistochemistry) in renal tissues. Several studies stated that after 24 h of MSCs infusion, only exceptionally scarce numbers of MSCs were found in the kidney, a pattern that essentially rules out the possibility that significant numbers of infused MSCs are retained in the kidney where they could physically replace lost kidney cells by transdifferentiation. This conclusion is furthermore supported by the fact that there was no intrarenal transdifferentiation events of MSCs within 3 days of administration, whereas occasional MSC-derived capillary endothelial cells were identified only after 5–7 days. From this, it could be deduced that the mechanisms that mediate the protective effects of MSC must be primarily paracrine. This is proved by their expression of several growth and antiapoptotic factors such as VEGF [45, 46] and IGF-I, Bax protein , all known to improve renal function in CRF, mediated by their antiapoptotic, mitogenic and other cytokine actions. Collectively, these as yet incompletely defined paracrine actions of MSCs result in the renal downregulation of proinflammatory cytokines IL-1β, TNF-α, and IFN-γ  and fibrogenic growth factors TGF-β  as well as iNOS, and upregulation of anti-inflammatory and organ-protective IL-10 [46, 49], as well as bFGF, TGF-α, and Bcl- 2.
Histopathological examination of renal tissue samples of DN & DN after MSCs injection groups supported these results.
In conclusion, MSC are capable of improving the kidney function and regenerating kidney tissues in DN rats most probably through their paracrine action via different growth factors such as VEGF, TGFβ & TNFα and antiapoptotic action via bcl2 & Bax genes.
- Sho-ichi Y, Takanori M: Advanced glycation end products, oxidative stress and diabetic nephropathy. Oxid Med Cell Longev. 2010, 3: 101-108. 10.4161/oxim.3.2.11148.View ArticleGoogle Scholar
- Forbes JM, Fukami K, Cooper ME: Diabetic nephropathy: where hemodynamics meets metabolism. Exp Clin Endocrinol Diabetes. 2007, 115: 69-84. 10.1055/s-2007-949721.View ArticlePubMedGoogle Scholar
- Schrijvers BF, De Vriese AS, Flyvbjerg A: From hyperglycemia to diabetic kidney disease: the role of metabolic, hemodynamic, intracellular factors and growth factors/cytokines. Endocr Rev. 2004, 25: 971-1010. 10.1210/er.2003-0018.View ArticlePubMedGoogle Scholar
- Navarro-Gonzalez JF, Mora-Fernandez C: The role of inflammatory cytokines in diabetic nephropathy. J Am SocNephrol. 2008, 19: 433-442.Google Scholar
- Weissman IL, Stem cells: Units of development, units of regeneration, and units in evolution. Cell. 2000, 100: 157-168. 10.1016/S0092-8674(00)81692-X.View ArticlePubMedGoogle Scholar
- Abdi R, Fiorina P, Adra CN, Atkinson M, Sayegh MH: Immunomodulation by mesenchymal stem cells: a potential therapeutic strategy for type 1 diabetes. Diabetes. 2008, 57: 1759-1767. 10.2337/db08-0180.PubMed CentralView ArticlePubMedGoogle Scholar
- Volarevic V, Al-Qahtani A, Arsenijevic N, Pajovic S, Lukic ML: Interleukin-1 receptor antagonist (IL-1Ra) and IL-1Ra producingmesenchymal stem cells as modulators of diabetogenesis. Autoimmunity. 2010, 43: 255-263. 10.3109/08916930903305641.View ArticlePubMedGoogle Scholar
- Hare JM, Traverse JH, Henry TD, Dib N, Strumpf RK, Schulman SP, Gerstenblith G, DeMaria AN, Denktas AE, Gammon RS, Hermiller JB, Reisman MA, Schaer GL, Sherman W: A randomized, double-blind, placebo-controlled, dose-escalation study of intravenous adult human mesenchymal stem cells (prochymal) after acute myocardial infarction. J Am Coll Cardiol. 2009, 54: 2277-2286. 10.1016/j.jacc.2009.06.055.PubMed CentralView ArticlePubMedGoogle Scholar
- Lee JS, Hong JM, Moon GJ, Ahn YH, Bang OY: A long-term follow-up study of intravenous autologous mesenchymal stem cell transplantation in patients with ischemic stroke. Stem Cells. 2010, 28: 1099-1106. 10.1002/stem.430.View ArticlePubMedGoogle Scholar
- Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, Moorman MA, Simonetti DW, Craig S, Marshak DR: Multilineage potential of adult human mesenchymal stem cells. Science. 1999, 284: 143-147. 10.1126/science.284.5411.143.View ArticlePubMedGoogle Scholar
- Zhang M, Mal N, Kiedrowski M, Chacko M, Askari AT, Popovic ZB, Koc ON, Penn MS: SDF-1 expression by mesenchymal stem cells results in trophic support of cardiac myocytes after myocardial infarction. FASEB J. 2007, 21: 3197-3207. 10.1096/fj.06-6558com.View ArticlePubMedGoogle Scholar
- Togel F, Weiss K, Yang Y, Hu Z, Zhang P, Westenfelder C: Vasculotropic, paracrine actions of infused mesenchymal stem cells are important to the recovery from acute kidney injury. Am J Physiol Renal Physiol. 2007, 292: F1626-35. 10.1152/ajprenal.00339.2006.View ArticlePubMedGoogle Scholar
- Zhou H, Tian H, Long Y, Zhang XX, Zhong L, Deng L, Chen XH, Li XQ: Mesenchymal stem cells transplantation mildly ameliorates experimental diabetic nephropathy in rats. Chin Med J. 2009, 122: 2573-2579.PubMedGoogle Scholar
- Abdel Aziz MT, Atta H, Mahfouz S, Yassin HM, Rashed LA, Sabry D, Abdel Wahab Z, Taha F, Abdel Aziz G, El Sayed AM, Sayed M: A study on the protective effect of bone marrow derived mesenchymal stem cells on chronic renal failure in rats. Stem cell studies. 2011, 1: e11-View ArticleGoogle Scholar
- Abdel Aziz MT, Atta H, Mahfouz S, Fouad HH, Roshdy NK, Ahmed HH, Rashed LA, Sabry D, Hassouna AA, Hasan NM: Therapeutic potential of bone marrow-derived mesenchymal stem cells on experimental liver cirrhosis. Clin Biochem. 2007, 40: 893-899. 10.1016/j.clinbiochem.2007.04.017.View ArticlePubMedGoogle Scholar
- Flyvbjerg: Putative pathophysiological role of growth factors and cytokines in experimental diabetic kidney disease. Diabetologia. 2000, 43: 1205-1223. 10.1007/s001250051515.View ArticlePubMedGoogle Scholar
- Galkina E, Ley K: Leukocyte recruitment and vascular injury in diabetic nephropathy. J Am Soc Nephrol. 2006, 17: 368-377. 10.1681/ASN.2005080859.View ArticlePubMedGoogle Scholar
- Chow FY, Nikolic-Paterson DJ, Atkins RC, Tesch GH: Macrophages in streptozotocin-induced diabetic nephropathy: potential role in renal fibrosis. Nephrol Dial Transplant. 2004, 19: 2987-2996. 10.1093/ndt/gfh441.View ArticlePubMedGoogle Scholar
- Chow FY, Nikolic-Paterson DJ, Ozols E, Atkins RC, Tesch GH: Intercellular adhesion molecule-1 deficiency is protective against nephropathy in type 2 diabetic db/db mice. J Am Soc Nephrol. 2005, 16: 1711-1722. 10.1681/ASN.2004070612.View ArticlePubMedGoogle Scholar
- Tansy MG, Szymkowski DE: The TNF superfamily in 2009: New pathways, new indications, and new drugs. Drug discovery Today. 2009, 2009 (14): 1082-1088.View ArticleGoogle Scholar
- Ortiz A, Bustos C, Alonso J, Alcazar R, Lopez-Armada MJ, Plaza JJ, González E, Egido J: Involvement of tumor necrosis factor-alpha in the pathogenesis of experimental, and human glomerulonephritis. Adv Nephrol Necker Hosp. 1995, 24: 53-77.PubMedGoogle Scholar
- Bertani T, Abbate M, Zoja C, Coma D, Perico N, Ghezzi P, Remuzzi G: Tumor necrosis factor induces glomerular damage in rabbit. Am J Pathol. 1989, 134: 419-430.PubMed CentralPubMedGoogle Scholar
- Laster SM, Wood JG, Gooding LR: Tumor necrosis factor can induce both apoptotic and necrotic forms of cell lysis. J Immunol. 1988, 141: 2629-2634.PubMedGoogle Scholar
- Boyle JJ, Weissberg PL, Bennett MR: Tumor necrosis factor-alpha promotes macrophage-induced vascular smooth muscle cell apoptosis by direct and autocrine mechanisms. Arterioscler Thromb Vasc Biol. 2003, 23: 1553-1558. 10.1161/01.ATV.0000086961.44581.B7.View ArticlePubMedGoogle Scholar
- Baud L, Perez J, Friedlander G, Ardaillou R: Tumor necrosis factor stimulates prostaglandin production and cyclic AMP levels in rat cultured mesangial cells. FEBS Lett. 1998, 239: 50-54.View ArticleGoogle Scholar
- Wojciak-Stothard B, Entwistle A, Garg R, Ridley AJ: Regulation of TNF-alpha-induced reorganization of the actin cytoskeleton and cell-cell junctions by Rho, Rac, and Cdc42 in human endothelial cells. J Cell Physiol. 1998, 176: 150-165. 10.1002/(SICI)1097-4652(199807)176:1<150::AID-JCP17>3.0.CO;2-B.View ArticlePubMedGoogle Scholar
- Raedke HH, Meier B, Topley N, Fluge J, Habermehl GG, Resch K: Interleukin 1-alpha and tumor necrosis factor-alpha induce oxygen radical production in mesangial cells. Kidney Int. 1990, 37: 767-775. 10.1038/ki.1990.44.View ArticleGoogle Scholar
- Sugimoto H, Shikata K, Wada J, Horiuchi S, Makino H: Advanced glycation end products–cytokine–nitric oxide sequence pathway in the development of diabetic nephropathy: Aminoguanidine ameliorates the overexpression of tumor necrosis factor-α and inducible nitric oxide synthase in diabetic rat glomeruli. Diabetologia. 1999, 42: 878-86. 10.1007/s001250051241.View ArticlePubMedGoogle Scholar
- Doi T, Vlassara H, Kirstein M, Yamada Y, Striker GE, Striker LJ: Receptor-specific increase in extracellular matrix production in mouse mesangial cells by advanced glycosylation end products is mediated via platelet-derived growth factor. Proc Natl Acad Sci USA. 1992, 89: 2873-7. 10.1073/pnas.89.7.2873.PubMed CentralView ArticlePubMedGoogle Scholar
- Brett J, Schmidt AM, Yan SD, Zou YS, Weidman E, Pinsky D, Nowygrod R, Neeper M, Przysiecki C, Shaw A, Migheli A, Stern D: Survey of the distribution of a newly characterized receptor for advanced glycation end products in tissues. Am J Pathol. 1993, 143: 1699-712.PubMed CentralPubMedGoogle Scholar
- Suzuki D, Toyoda M, Yamamoto N, Miyauchi M, Katoh M, Kimura M, Maruyama M, Honma M, Umezono T, Yagame M: Relationship between the expression of advanced glycation end-products (AGE) and the receptor for AGE (RAGE) mRNA in diabetic nephropathy. Intern Med. 2006, 45: 435-41. 10.2169/internalmedicine.45.1557.View ArticlePubMedGoogle Scholar
- Vlassara H, Brownlee M, Manogue KR, Dinarello CA, Pasagian A: Cachectin/TNF and IL-1 induced by glucose-modified proteins: role in normal tissue remodeling. Science. 1988, 240: 1546-8. 10.1126/science.3259727.View ArticlePubMedGoogle Scholar
- Rashid G, Benchetrit S, Fishman D, Bernheim J: Effect of advanced glycation end-products on gene expression and synthesis of TNF-α and endothelial nitric oxide synthase by endothelial cells. Kidney Int. 2004, 66: 1099-106. 10.1111/j.1523-1755.2004.00860.x.View ArticlePubMedGoogle Scholar
- Matem T, Melek Ö: The effects of angiotensin-II receptor blockers on podocyte damage and glomerular apoptosis in a rat model of experimental streptozotocin-induced diabetic nephropathy. Acthis. 2011, 12: 003-Google Scholar
- Cornacchia F, Fornoni A, Plati AR, Thomas A, Wang Y, Inverardi L, Striker LJ, Striker GE: Glomerulosclerosis is transmitted by bone –marrow drivedmesangial progenitors. J Clin Invest. 2001, 108: 1649-1656. 10.1172/JCI200112916.PubMed CentralView ArticlePubMedGoogle Scholar
- Poulsom R, Forbes SJ, Hodivala-DHke K, Ryan E, Wyles S, Navaratnarasah S, Jeffery R, Hunt T, Alison M, Cook T, Pusey C, Wright NA: Bone marrow contributes to renal parenchymal turnover and regeneration. J Pathol. 2001, 195: 229-235. 10.1002/path.976.View ArticlePubMedGoogle Scholar
- Anglani F, Forino M, Del Prete D, Tosetto E, Torregrossa R, D’Angelo A: In search of adult renal stem cells. J Cell Mol Med. 2004, 8: 474-487. 10.1111/j.1582-4934.2004.tb00472.x.View ArticlePubMedGoogle Scholar
- Morigi M, Introna M, Imberti B, Corna D, Abbate M, Rota C, Rottoli D, Benigni A, Perico N, Zoja C, Rambaldi A, Remuzzi A, Remuzzi G: Human bone marrow mesenchymal stem cells accelerate recovery of acute renal injury and prolong survival in mice. Stem Cells. 2008, 26: 2075-2082. 10.1634/stemcells.2007-0795.View ArticlePubMedGoogle Scholar
- Ling L, Truong P, Igarashi P, Lin F: Renal and bone marrow cells fuse after renal ischemic injury. J Am Soc Nephrol. 2007, 18: 3067-3077. 10.1681/ASN.2007030284.View ArticleGoogle Scholar
- Rookmaaker MB, Verhaar MC, de Boer HC, Goldschmeding R, Joles JA, Koomans HA, Gröne HJ, Rabelink TJ: Met-RANTES reduces endothelial progenitor cell homing to activated (glomerular) endothelium in vitro and in vivo. Am J Physiol Renal Physiol. 2007, 293: F624-630. 10.1152/ajprenal.00398.2006.View ArticlePubMedGoogle Scholar
- Ito T, Suzuki A, Okabe M, Imai E, Hori M: Application of bone marrow-derived stem cells in experimental nephrology. Exp Nephrol. 2001, 9: 444-450. 10.1159/000052644.View ArticlePubMedGoogle Scholar
- Togel F, Hu Z, Weiss K, Isaac J, Lange C, Westenfelder C: Administered mesenchymal stem cells protect against ischemic acute renal failure through differentiation-independent mechanisms. Am J Physiol Renal Physiol. 2005, 289: F31-42. 10.1152/ajprenal.00007.2005.View ArticlePubMedGoogle Scholar
- Duffield JS, Bonventre JV: Kidney tubular epithelium is restored withoutreplacement with bone marrow-derived cells during repair after ischemic injury. Kidney Int. 2005, 68: 1956-1961. 10.1111/j.1523-1755.2005.00629.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Chishti AS, Sorof JM, Brewer ED, Kale AS: Long-term treatment of focal segmental glomerulosclerosis in children with cyclosporine given as a single daily dose. Am J Kidney Dis. 2001, 38: 754-760. 10.1053/ajkd.2001.27692.View ArticlePubMedGoogle Scholar
- Shimizu A, Masuda Y, Mori T, Kitamura H, Ishizaki M, Sugisaki Y, Fukuda Y: Vascular endothelial growth factor165 resolves glomerular inflammation and accelerates glomerular capillary repair in rat anti-glomerular basement membrane glomerulonephritis. J Am Soc Nephrol. 2004, 15: 2655-2665. 10.1097/01.ASN.0000141038.28733.F2.View ArticlePubMedGoogle Scholar
- Abdel Aziz MT, Wassef MA, Rashed LA, Mhfouz S, Omar N, Elsebaie MM: Mesenchymal stem cells therapy in acute renal failure: possible role of hepatocyte growth factor. J Stem Cell Res Ther. 2011, 1: 3-Google Scholar
- Deng J, Kohda Y, Chiao H, Wang Y, Hu X, Hewitt SM, Miyaji T, McLeroy P, Nibhanupudy B, Li S, Star RA: Interleukin-10 inhibitsischemic and cisplatin-induced acute renal injury. Kidney Int. 2001, 60: 2118-2128. 10.1046/j.1523-1755.2001.00043.x.View ArticlePubMedGoogle Scholar
- Semedo P, Palasio CG, Oliveira CD, Feitoza CQ, Gonçalves GM, Cenedeze MA, Wang PM, Teixeira VP, Reis MA, Pacheco-Silva A, Câmara NO: Early modulation of inflammation by mesenchymal stem cell after acute kidney injury. Int Immunopharmacol. 2009, 9: 677-682. 10.1016/j.intimp.2008.12.008.View ArticlePubMedGoogle Scholar
- Zhang Y, Ye C, Wang G, Gao Y, Tan K, Zhuo Z, Liu Z, Xia H, Yang D, Li P: Kidney-targeted transplantation of mesenchymal stem cells by ultrasound-targeted microbubble destruction promotes kidney repair in diabetic nephropathy rats. Biomed Res Int. 2013, 2013: 526367-PubMed CentralPubMedGoogle Scholar
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