- Meeting abstract
- Open Access
Evaluation of acute oral toxicity of red alga (Gracilaria domingensis sonder ex kützing) in mice C57BL/6
- José Ytalo Gomes da Silva1Email author,
- Marcelo Oliveira Holanda1,
- Carla Laine Silva Lima1,
- Paula Alves Salmito Rodrigues1,
- Erlândia Alves Magalhães Queiroz1,
- Raquel Teixeira Terceiro Paim1,
- Thais Vital de Freitas1,
- Juliana Barbosa Dantas1,
- Sandra Machado Lira1,
- Natalia do Vale Canabrava1,
- Mariana de Freitas Moreira1,
- Bruno Bezerra Silva1,
- Chayane Gomes Marques1,
- Julianne do Nascimento Sales1,
- Arnaldo Solheiro Bezerra1,
- Emanuele Silva de Sousa1,
- Rafaela Valesca Rocha Bezerra Sousa1,
- Lia Magalhães de Almeida1,
- Francisca Noélia Pereira Mendes1,
- Icaro Gusmão Pinto Vieira1 and
- Maria Izabel Florindo Guedes1
© da Silva et al. 2015
- Published: 11 November 2015
- Plant Extract
- Acute Toxicity
- Toxicity Test
- Behavioral Observation
- Administration Route
Many species of plants have been used pharmacologically to treat the symptoms of diabetes mellitus. However, it is influenced by the toxicity of the plant extract used in the preparation, method of preparation and administration route, so it is important to identify potential risks regarding the toxicity of the product to be used. The red algae (Gracilaria domingensis) is a source of minerals, vitamins, fiber and low in lipids, and may contain anti-hyperglycemic activity, contributing thus in control of DM. Therefore, it becomes necessary to carry out acute toxicity tests to evaluate the red algae is safe for therapeutic use.
To evaluate the acute toxicity of red algae in healthy mice.
A flour of algae was obtained by drying in an oven at 45 ° C with forced air circulation, followed by grinding. Twelve adult females C57BL/6 mice, weighing 22-25g, were used in this study. The experimental protocol of this study was submitted and accepted by the Ethics in Animal Research Committee (EARC) with number 90/10. The mice were divided in 2 groups (n=6) and fasted for 4 h. After this period, was administered by gavage, saline (1 mL/kg) to the control group, and the solution of algae at a dose of 2000 mg/kg to the test group, followed by behavioral observation of the animals at 30, 60, 90, 120, 150, 180, 210, 240, 270 and 300. After twelve days the animals were euthanized for removal and analysis of the relative weight vital organs to check for acute toxicity. The analysis of the significance of differences between the data was performed using nonparametric Mann Whitney, considering significant Results that had p <0.05.
Given the above Results, it is concluded that the administered solution of the red algae does not have toxic effects and is safe for therapeutic use.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.