- Open Access
Precocious markers of cardiovascular risk and vascular damage in apparently healthy women with previous gestational diabetes
© Zajdenverg et al.; licensee BioMed Central Ltd. 2014
- Received: 1 December 2013
- Accepted: 19 May 2014
- Published: 26 May 2014
Previous gestational diabetes mellitus (pGDM) indicates future risk for type 2 diabetes (T2DM). Insulin resistance (IR) may precede T2DM in many years and is associated with an increased risk for cardiovascular diseases.
This study aims to identify endothelial dysfunction and cardiovascular risk factors in women with pGDM.
This cross-sectional analysis included 45 non diabetic women, 20 pGDM and 25 controls, at least one year after delivery. Body mass index (BMI), abdominal circumference (AC), blood pressure, serum lipids, liver enzymes, uric acid, nonesterified fatty acids, C-reactive protein and plasma glucose, insulin, fibrinogen and plasminogen activator inhibitor 1 were measured. HOMA IR and β were calculated. Pre and post induced ischemia videocapillaroscopy was performed in hand nailfold to evaluate microvascular morphologic aspect and functional response.
AC and fasting glucose were significantly higher in pGDM (p = 0.01 and p = 0.002 respectively). Women with pGDM and BMI < 25 kg/m2 had significantly higher levels of fasting insulin and HOMA IR than controls (p = 0.008 and 0.05 respectively). Abnormal morphologic findings were more frequent and papillae rectification were 3.3 times more prevalent in pGDM (p = 0.003). Other microvascular parameters did not differ between groups.
Cardiovascular risk factors and a microcirculation abnormality (papillae rectification) were significantly increased in young non-diabetic women with pGDM.
- Gestational diabetes
- Cardiovascular risk
Gestational diabetes mellitus (GDM) is a heterogeneous disorder defined as glucose intolerance first recognized during pregnancy . This metabolic abnormality usually resolves immediately post partum, but implies in a higher risk of future type 2 diabetes mellitus (T2DM). In the vast majority of cases, the pathogenesis of GDM resembles that of T2DM. In both conditions, the β cell reserve is unable to counterbalance the insulin resistance. During pregnancy, this increased demand is caused by placental hormones. It has been suggested that GDM and T2DM should be taken as one single entity, although clinically evident in different lifetime periods .
T2DM is a known risk factor for cardiovascular disease (CVD) and insulin resistance (IR) has been implicated in this association . The mechanisms involved in this link are multiple and complex. Chronic hyperglycemia may directly participate in the development of CVD  and other conditions frequently seen in patients with T2DM may also contribute to the increased risk, such as hypertension, dyslipidemia, altered fibrinolysis and obesity [5, 6]. Endothelial dysfunction and chronic sub-clinical or low-grade inflammation are closely associated with obesity and insulin resistance and could be involved in the pathogenesis of T2DM and CVD. Increased levels of acute phase proteins such as C-reactive protein (CRP), interleukin 6 (IL6) and sialic acid have been shown in patients with T2DM and insulin resistance [7, 8] and are known to be associated with CVD –. This low-grade chronic inflammation at the level of the vessel wall appears to mediate all stages of atherosclerosis and is a reflection of endothelial dysfunction. Capillary morphological and functional abnormalities have also been described in patients with T2DM during videocapillaroscopy (VC) [12–14], due to endothelial dysfunction, increased plasma viscosity, autonomic neuropathy and high vascular permeability [15, 16].
Groups at risk for T2DM such as women with pGDM usually have more insulin resistance than the healthy general population, which can start many years before the appearance of any derangement in glucose homeostasis [17, 18]. Beta cell dysfunction is also important for the development of T2DM in these individuals . Ryan et al. have shown that women with previous GDM even with normal post-pregnancy oral glucose tolerance test (OGTT) had both insulin secretion and action defects in the post-conception period , although T2DM usually develops only years after pregnancy in this population. Therefore, this group might be considered in a very early stage of the natural history of T2DM and may offer a unique opportunity of studying abnormalities that appear early in the process.
It is still unclear how early a higher CVD risk appears in the natural history of T2DM. Women with previous GDM (pGDM) and a normal post-pregnancy OGTT already have a higher risk than the general population . Endothelial dysfunction and sub-clinical inflammation have often been reported in individuals at risk for T2DM in general, which would contribute to the development of CVD . In the specific subgroup of women with pGDM, few and contrasting data have reported vascular endothelial dysfunction and increased serum levels of inflammatory markers. Insulin resistance itself and increased abdominal fat could be implicated in these phenomena, although the possibility of a specific genetically determined vascular derangement has also been suggested [9, 11, 21–24]. The aim of this study was to identify cardiovascular risk factors and microvascular abnormalities diagnosed through videocapillaroscopy in apparently healthy women with pGDM from a multi-ethnic population.
We have performed a cross-sectional analysis of cardiovascular risk factors and endothelial function in women with pGDM and those with a past history of normal glucose tolerance during pregnancy. The protocol was approved by the Local Ethics Committee and informed consent was obtained from all subjects. Ninety six women who had the diagnosis of GDM between 24 and 30th week of pregnancy at least 1 year before the study were invited to participate. The diagnosis of GDM was established with a 2 step- protocol according to the previous ADA criteria . All patients were followed during and after pregnancy by the same medical team in the Diabetes and Pregnancy outpatient unit of Maternidade Escola da Universidade Federal do Rio de Janeiro. Women with T2DM, impaired fasting glucose or glucose intolerance according to OMS criteria , current pregnancy, menopause, use of vasoactive, antilipemic or antidiabetics drugs, vascular, kidney, liver, dermatological and infectious diseases were excluded from the analysis. From the 37 women with pGDM that accepted to participate, 20 were included. Twenty five healthy women selected in the same outpatient unit who had delivered 1 year or more before the study without pGDM, history of fetal macrosomia, menopause, use of vasoactive or antilipemic drugs and arterial hypertension were included as controls.
Maternal age in the labor, weight before pregnancy and in the third trimester, parity and previous abortions history were obtained in the medical charts; family history of diabetes, educational status, ethnic group, height, current weight, abdominal circumference and blood pressure were registered. Fasting capillary blood glucose (12–14 hours) was measured and subjects with results ≥ 140 mg/dl were excluded. All other patients underwent a 75 grams 2 hour oral glucose tolerance test. Serum creatinine, whole and HDL cholesterol, tryglicerides, liver enzymes, uric acid, nonesterified fatty acids, C-reactive protein and plasma insulin, fibrinogen and plasminogen activator inhibitor 1 were measured. Plasma insulin was measured with a fluoroimmunoassay (Auto Delfia kit, Turku, Finland). NEFA were measured with an enzymatic method (Wako, Richmond, VA); normal range in fasting state: 0.09-0.6 mmol/l). PAI-1 was measured with a Chromolize assay (Biopool International,Ventura, CA) wich reference interval for subjects between 20 to 49 years old was < 14.4 U/ml. Homeostasis model assessment (HOMA) IR and β were calculated. It was applied a logarithmic function of the HOMAβ in order to get a normal distribution of those values.
For the statistical analysis, Mann Whitney U and Fisher exact tests were used to compare data between groups. Spearman correlation was used to test for association between continuous variables. All tests were two-tailed, with α = 0.05 for the main hypothesis.
Characteristics of the study group
Previous GDM Mean (SD)
Controls Mean (SD)
Mean age (in years)
Educational level (years)
Mean number of labors
Mean number of pregnancies
Age at conception (years)
Nursing period (months)
Body mass index
29.7 (5.9) Kg/m2
26.5 (6.19) Kg/m2
Abdominal circumference (AC)
102.3 (16.3) cm
91.2 (16.2) cm
Systolic blood pressure
114.5 (17.9) mmHg
110.8 (16.5) mmHg
Diastolic blood pressure
76.5 (11.8) mmHg
72.8 (12.0) mmHg
Previous GDM Mean (SD)
Controls Mean (SD)
Mean fasting glucose
83.2 (13.0) mg/dl
71.6 (9.4) mg/dl
0.72 (0.1) mg/dl
0.67 (0.1) mg/dl
24.4 (5.2) U/l
24.9 (4.9) U/l
35.6 (7.8) U/l
40.8 (13.6) U/l
3.9 (0.7) mg/dl
3.7 (0.8) mg/dl
194.0 (33.8) mg/dl
185.6 (43.8) mg/dl
46.5 (11.4) mg/dl
53.6 (11.0) mg/dl
130.0 (32.3) mg/dl
114.3 (34.9) mg/dl
87.0 (38.1) mg/dl
88.1 (63.1) mg/dl
0.47 (0.19) mmol/l
0.55 (0.23) mmol/l
0.34 (0.23) mg/dl
0.40 (0.49) mg/dl
279.5 (58.7) mg/dl
297.1 (84.7) mg/dl
12.1 (9.6) U/ml
16.0 (12.0) U/ml
VARIABLE Mean/median (SD)
Previous GDM (Group 1)
Controls (Group 2)
BMI < 25
BMI ≥ 25
AC < 88 cm
AC ≥ 88 cm
BMI < 25
BMI ≥ 25
Abnormal findings at basal videocapillaroscopy
Previous GDM (Group 1) (%)
Controls (Group 2) (%)
HR (IC 95%)
Disordered distribution of loops
8.44 (0.37- 188.63)
Loops with aberrant form
1.72 (0.40- 7.32)
Tortuous transverse segment
2.27 (0.50- 10.25)
Enlarged transverse segment
2.42 (0.65- 9.06)
4.20 (0.86- 20.34)
8.36 (1.97- 35.47)
Basal and dynamic videocapillaroscopy results
p = 0.24
p = 0.15
p = 0.71
p = 0.28
This study intended to investigate possible CVD risk factors and microvascular abnormalities diagnosed through VC in women with pGDM and normal OGTT, when compared to a control group. Several interesting findings were seen. First, BMI did not differ between groups, but a higher AC was observed in those with pGDM than in controls. Verma et al. also reported similar BMI in women with and without pGDM from six to ten years after the delivery, despite higher BMI observed in pGDM in the fourth and fifth years after pregnancy . In our study group, the lack of difference was observed in individuals tested 43.26 months, in average, after their labor. We found a correlation between AC and weight gain after but not during pregnancy. This finding may represent changes in fat distribution associated with the aging process or parity. A limitation in this study is the inability to determine if AC differences between groups would be present prior to pregnancy. Branchtein L et al. have shown a significant correlation between AC and glycemic levels in the OGTT in 1025 women evaluated between 21th and 28th weeks of pregnancy .
FG were higher in subjects with pGDM than others, which was more evident in those with normal BMI and AC < 88 cm. Similar differences in FG were observed previously [18, 28]–. There is evidence that higher FG levels, even within the normal range, may represent a marker of increased risk for CVD. Pallardo F et al. have reported an association between central obesity, triglycerides levels and arterial blood pressure with FG but not with glucose intolerance . HOMA IR was also higher in women with pGDM than others, indicating that this condition is associated with increased insulin resistance, even in the absence of OGTT abnormalities. This finding was more striking in individuals with normal BMI, which leads us to believe that insulin resistance in this group occurs independently of the presence of overweight. No correlations were found between HOMA IR and parity, weight gain during pregnancy or weight variation after labor. This may indicate that insulin resistance precedes these environmental factors possibly associated with metabolic abnormalities. No differences between groups were found in the HOMAβ, what suggests that β cell dysfunction actually develops later in the natural history of T2DM. Abnormalities in insulin sensitivity but not secretion in pGDM women have been shown before [28, 32].
No significant differences in the lipid profile were seen between groups, except for a tendency towards lower levels of HDL in those with pGDM, when compared to controls. Low levels of HDL are not only markers of insulin resistance, but may also of a lack of anti-atherogenic effects . We cannot exclude that the lack of differences between groups in free fatty acid (FFA) levels may be due to the fact that only a basal measurement was performed. Indeed, Kousta E et al. did not find differences in basal FFA levels between women with pGDM and controls, but the former exhibited lower suppression of FFA than the latter after venous glucose tolerance tests .
A few authors have shown that pGDM individuals, despite being currently free from metabolic abnormalities, have higher values of markers of endothelial dysfunction, such as E-selectin and intercellular adhesion molecule 1 or ICAM-1 , but there is not a consensus. Higher levels of CRP and sialic acid, both markers of chronic low-grade inflammation, were also reported in women with pGDM [28, 36, 37]. In our study, similar levels of CRP were found in both groups and in all women this marker was within the normal range. No association was found between CRP and BMI, which might be due to the low variation of CRP levels in the whole sample, as the association between CRP and obesity has been shown before . Kim et al. also found no differences in CRP and other inflammatory markers (ferritin and leucocyte count) among women with or without previous GDM .
Differently from Heittritter et al., who have shown higher levels of PAI-1 in women with pGDM, we did not see any difference between groups nor any association between this marker with BMI or AC. Although these results might be due to the small sample size, other hypothesis to explain this finding would be the multiethnic nature of our study group. Solano MP et al. detected differences in the association of PAI 1 and insulin resistance markers in women from different ethnic backgrounds. In that analysis, Afro-descendants women did not show any association between PAI-1 and visceral adiposity .
Fibrinogen levels did not differ between groups and 95% of the subjects had normal levels of this marker. Di Bennedetto et al., on the other hand, found higher levels of fibrinogen in individuals with pGDM than controls . It is still unclear if fibrinogen is implicated in the pathogenesis of CVD or is a marker that reflects its appearance, but our data suggests that its raise is not an early marker of insulin resistance.
VC has shown a higher number of papillae rectification in the nail capillary bed in women with pGDM than others. Papillae rectification, disordered distribution and shortening of capillary loops were approximately 8 times more prevalent and presence of microectasias was 4 times more prevalent in women with pGDM. Hofstee et all recently show that all quantitative and certain qualitative parameters are highly reliable in terms of inter- and intra-observer agreement in VC .
The increase in capillary permeability is the first abnormality in diabetic microangiopathy. Abnormalities in the shape of the papillae, shortening of capillary loops and edema are markers of increased capillary permeability . Microectasia is a later finding, associated with ischemia and endothelial cell damage in the capillary wall . To our knowledge, this is the first study to identify VC abnormalities in women with pGDM. The prevalence of morphologic abnormalities in women with pGDM in our study group was slightly higher than that described by Halfoun et al. in healthy first degree relatives of patients with T2DM . This emphasizes the importance of this finding as an early microvascular alteration in this population at risk for T2DM. A few authors have also shown early microvascular abnormalities in women with pGDM. Shannon et al. studied nondiabetic women with prior GDM and showed subclinical inflammation, hypoadiponectinemia, and early vascular dysfunction in this population suggesting an increased risk of developing CVD  . Anastasiou et al. have observed a endothelium-dependent vasodilation in the brachial artery , although a smaller study did not find any abnormality through the same technique and in vascular reactivity in the skin . Aortic function assessed by ultrasonography and small vessel performance assessed by iontophoresis and laser Doppler flowmetry was also reported in these individuals. Hu et al. reported an endothelium-dependent and -independent vasodilatation impaired response of the microcirculation in women with pGDM group when compared to controls . Banerjee et al. have shown that overweight women with disglycemia during pregnancy had normal vascular structure and stiffness, but detectable progressively impaired endothelium-dependent function at 2 years follow-up .
Those early vascular abnormalities might be a result of a “hyperglycemic legacy”. Alternatively, it is possible that some intrinsic defects in vascular function may be operating in insulin-resistant individuals, even before OGTT abnormalities become evident.
The absence of significant differences between groups in some biochemical parameters and endothelial function might be due to the characteristics of our study group, composed by healthy young women. Caballero et al. have shown important correlations between markers of endothelial damage and age in a study group that comprised relatives of patients with T2DM older than our subjects studied .
To summarize, women with pGDM, even with normal OGTT, have subtle yet significant differences in some CVD risk factors when compared to others, as well as microvascular abnormalities (papillae rectification). Although most of these individuals do not develop T2DM, they are exposed to clinical and laboratory risk factors to CVD at a young age. These women should be given counselling and appropriate advice on lifestyle, in order to decrease their risk for CVD and T2DM. Longer-term studies are necessary to verify if a pGDM would represent a marker of future increased cardiac morbidity and define the role of lifestyle and/or pharmacological interventions in these subjects.
- American Diabetes A: Diagnosis and classification of diabetes mellitus. Diabetes Care. 2004, 27 (Suppl 1): S5-S10.Google Scholar
- Luoto RM, Kinnunen TI, Aittasalo M, Ojala K, Mansikkamäki K, Toropainen E, Kolu P, Vasankari T: Prevention of gestational diabetes: design of a cluster-randomized controlled trial and one-year follow-up. BMC Pregnancy Childbirth. 2010, 10: 39-10.1186/1471-2393-10-39.PubMed CentralView ArticlePubMedGoogle Scholar
- Fox CS: Cardiovascular disease risk factors, type 2 diabetes mellitus, and the Framingham heart study. Trends Cardiovasc Med. 2010, 20 (3): 90-95. 10.1016/j.tcm.2010.08.001.PubMed CentralView ArticlePubMedGoogle Scholar
- King GL, Wakasaki H: Theoretical mechanisms by which hyperglycemia and insulin resistance could cause cardiovascular diseases in diabetes. Diabetes Care. 1999, 22 (Suppl 3): C31-C37.PubMedGoogle Scholar
- Bloomgarden ZT: Obesity, hypertension, and insulin resistance. Diabetes Care. 2002, 25 (11): 2088-2097. 10.2337/diacare.25.11.2088.View ArticlePubMedGoogle Scholar
- Caballero AE: Endothelial dysfunction, inflammation, and insulin resistance: a focus on subjects at risk for type 2 diabetes. Curr Diab Rep. 2004, 4 (4): 237-246. 10.1007/s11892-004-0074-9.View ArticlePubMedGoogle Scholar
- Sriharan M, Reichelt AJ, Opperman ML, Duncan BB, Mengue SS, Crook MA, Schmidt MI: Total sialic acid and associated elements of the metabolic syndrome in women with and without previous gestational diabetes. Diabetes Care. 2002, 25 (8): 1331-1335. 10.2337/diacare.25.8.1331.View ArticlePubMedGoogle Scholar
- Festa A, D'Agostino R, Howard G, Mykkänen L, Tracy RP, Haffner SM: Chronic subclinical inflammation as part of the insulin resistance syndrome: the insulin resistance atherosclerosis study (IRAS). Circulation. 2000, 102 (1): 42-47. 10.1161/01.CIR.102.1.42.View ArticlePubMedGoogle Scholar
- Hwang SJ, Ballantyne CM, Sharrett AR, Smith LC, Davis CE, Gotto AM, Boerwinkle E: Circulating adhesion molecules VCAM-1, ICAM-1, and E-selectin in carotid atherosclerosis and incident coronary heart disease cases: the atherosclerosis risk in communities (ARIC) study. Circulation. 1997, 96 (12): 4219-4225. 10.1161/01.CIR.96.12.4219.View ArticlePubMedGoogle Scholar
- Ridker PM, Hennekens CH, Buring JE, Rifai N: C-reactive protein and other markers of inflammation in the prediction of cardiovascular disease in women. N Engl J Med. 2000, 342 (12): 836-843. 10.1056/NEJM200003233421202.View ArticlePubMedGoogle Scholar
- Kondo K, Kitagawa K, Nagai Y, Yamagami H, Hashimoto H, Hougaku H, Hori M: Associations of soluble intercellular adhesion molecule-1 with carotid atherosclerosis progression. Atherosclerosis. 2005, 179 (1): 155-160. 10.1016/j.atherosclerosis.2004.09.018.View ArticlePubMedGoogle Scholar
- Pazos-Moura CC, Moura EG, Bouskela E, Torres-Filho IP, Breitenbach MM: Nailfold capillaroscopy in diabetes mellitus: morphological abnormalities and relationship with microangiopathy. Braz J Med Biol Res. 1987, 20 (6): 777-780.PubMedGoogle Scholar
- Mouthon JM, Bosquet F, Grimaldi A, Wechsler B, Thervet F, Godeau P: Diabetic microangiopathy. Role of capillaroscopy. Presse Med. 1989, 18 (33): 1647-1650.PubMedGoogle Scholar
- Josa G, Salvi P, Zoli I, Battistini G, Pretolani E: [Capillaroscopy and diabetic microangiopathies]. J Mal Vasc. 1989, 14 (1): 55-56.PubMedGoogle Scholar
- Fahrig C, Breitinger L, Heidrich H: Vital capillary microscopic findings in the nailfold of patients with diabetes mellitus. Vasa. 2000, 29 (4): 258-263. 10.1024/0301-15184.108.40.2068.View ArticlePubMedGoogle Scholar
- Halfoun VL, Pires ML, Fernandes TJ, Victer F, Rodrigues KK, Tavares R: Videocapillaroscopy and diabetes mellitus: area of transverse segment in nailfold capillar loops reflects vascular reactivity. Diabetes Res Clin Pract. 2003, 61 (3): 155-160. 10.1016/S0168-8227(03)00111-6.View ArticlePubMedGoogle Scholar
- Kahn BB, Flier JS: Obesity and insulin resistance. J Clin Invest. 2000, 106 (4): 473-481. 10.1172/JCI10842.PubMed CentralView ArticlePubMedGoogle Scholar
- Gerich JE: Contributions of insulin-resistance and insulin-secretory defects to the pathogenesis of type 2 diabetes mellitus. Mayo Clin Proc. 2003, 78 (4): 447-456. 10.4065/78.4.447.View ArticlePubMedGoogle Scholar
- Ryan EA, Imes S, Liu D, McManus R, Finegood DT, Polonsky KS, Sturis J: Defects in insulin secretion and action in women with a history of gestational diabetes. Diabetes. 1995, 44 (5): 506-512. 10.2337/diab.44.5.506.View ArticlePubMedGoogle Scholar
- Ekelund M, Shaat N, Almgren P, Groop L, Berntorp K: Prediction of postpartum diabetes in women with gestational diabetes mellitus. Diabetologia. 2010, 53 (3): 452-457. 10.1007/s00125-009-1621-3.View ArticlePubMedGoogle Scholar
- Gokce N, Keaney JF, Hunter LM, Watkins MT, Nedeljkovic ZS, Menzoian JO, Vita JA: Predictive value of noninvasively determined endothelial dysfunction for long-term cardiovascular events in patients with peripheral vascular disease. J Am Coll Cardiol. 2003, 41 (10): 1769-1775. 10.1016/S0735-1097(03)00333-4.View ArticlePubMedGoogle Scholar
- Lim SC, Caballero AE, Smakowski P, LoGerfo FW, Horton ES, Veves A: Soluble intercellular adhesion molecule, vascular cell adhesion molecule, and impaired microvascular reactivity are early markers of vasculopathy in type 2 diabetic individuals without microalbuminuria. Diabetes Care. 1999, 22 (11): 1865-1870. 10.2337/diacare.22.11.1865.View ArticlePubMedGoogle Scholar
- Ridker PM, Buring JE, Cook NR, Rifai N: C-reactive protein, the metabolic syndrome, and risk of incident cardiovascular events: an 8-year follow-up of 14 719 initially healthy American women. Circulation. 2003, 107 (3): 391-397. 10.1161/01.CIR.0000055014.62083.05.View ArticlePubMedGoogle Scholar
- Halcox JP, Schenke WH, Zalos G, Mincemoyer R, Prasad A, Waclawiw MA, Nour KR, Quyyumi AA: Prognostic value of coronary vascular endothelial dysfunction. Circulation. 2002, 106 (6): 653-658. 10.1161/01.CIR.0000025404.78001.D8.View ArticlePubMedGoogle Scholar
- Alberti KG, Zimmet PZ: Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med. 1998, 15 (7): 539-553. 10.1002/(SICI)1096-9136(199807)15:7<539::AID-DIA668>3.0.CO;2-S.View ArticlePubMedGoogle Scholar
- Verma A, Boney CM, Tucker R, Vohr BR: Insulin resistance syndrome in women with prior history of gestational diabetes mellitus. J Clin Endocrinol Metab. 2002, 87 (7): 3227-3235. 10.1210/jcem.87.7.8684.View ArticlePubMedGoogle Scholar
- Branchtein L, Schmidt MI, Mengue SS, Reichelt AJ, Matos MC, Duncan BB: Waist circumference and waist-to-hip ratio are related to gestational glucose tolerance. Diabetes Care. 1997, 20 (4): 509-511. 10.2337/diacare.20.4.509.View ArticlePubMedGoogle Scholar
- Heitritter SM, Solomon CG, Mitchell GF, Skali-Ounis N, Seely EW: Subclinical inflammation and vascular dysfunction in women with previous gestational diabetes mellitus. J Clin Endocrinol Metab. 2005, 90 (7): 3983-3988. 10.1210/jc.2004-2494.View ArticlePubMedGoogle Scholar
- Albareda M, Caballero A, Badell G, Piquer S, Ortiz A, de Leiva A, Corcoy R: Diabetes and abnormal glucose tolerance in women with previous gestational diabetes. Diabetes Care. 2003, 26 (4): 1199-1205. 10.2337/diacare.26.4.1199.View ArticlePubMedGoogle Scholar
- Kim C, Cheng YJ, Beckles GL: Cardiovascular disease risk profiles in women with histories of gestational diabetes but without current diabetes. Obstet Gynecol. 2008, 112 (4): 875-883. 10.1097/AOG.0b013e31818638b5.PubMed CentralView ArticlePubMedGoogle Scholar
- Pallardo F, Herranz L, Garcia-Ingelmo T, Grande C, Martin-Vaquero P, Jañez M, Gonzalez A: Early postpartum metabolic assessment in women with prior gestational diabetes. Diabetes Care. 1999, 22 (7): 1053-1058. 10.2337/diacare.22.7.1053.View ArticlePubMedGoogle Scholar
- JH M: Follow-up studies in women with gestational diabetes mellitus: the experience at Los Angeles Country/University of Southern California Medical Center. Gestational diabetes. Edited by: Weiss PAM, Counstan DR. 1988, Vienna: Springer, 191-198.Google Scholar
- Mineo C, Shaul PW: HDL stimulation of endothelial nitric oxide synthase: a novel mechanism of HDL action. Trends Cardiovasc Med. 2003, 13 (6): 226-231. 10.1016/S1050-1738(03)00098-7.View ArticlePubMedGoogle Scholar
- Kousta E, Lawrence NJ, Godsland IF, Penny A, Anyaoku V, Millauer BA, Cela E, Johnston DG, Robinson S, McCarthy MI: Insulin resistance and beta-cell dysfunction in normoglycaemic European women with a history of gestational diabetes. Clin Endocrinol (Oxf). 2003, 59 (3): 289-297. 10.1046/j.1365-2265.2003.01820.x.View ArticleGoogle Scholar
- Bo S, Monge L, Macchetta C, Menato G, Pinach S, Uberti B, Pagano G: Prior gestational hyperglycemia: a long-term predictor of the metabolic syndrome. J Endocrinol Invest. 2004, 27 (7): 629-635. 10.1007/BF03347494.View ArticlePubMedGoogle Scholar
- Di Cianni G, Lencioni C, Volpe L, Ghio A, Cuccuru I, Pellegrini G, Benzi L, Miccoli R, Del Prato S: C-reactive protein and metabolic syndrome in women with previous gestational diabetes. Diabetes Metab Res Rev. 2007, 23 (2): 135-140. 10.1002/dmrr.661.View ArticlePubMedGoogle Scholar
- Di Benedetto A, Russo GT, Corrado F, Di Cesare E, Alessi E, Nicocia G, D'Anna R, Cucinotta D: Inflammatory markers in women with a recent history of gestational diabetes mellitus. J Endocrinol Invest. 2005, 28 (1): 34-38.View ArticlePubMedGoogle Scholar
- Hak AE, Stehouwer CD, Bots ML, Polderman KH, Schalkwijk CG, Westendorp IC, Hofman A, Witteman JC: Associations of C-reactive protein with measures of obesity, insulin resistance, and subclinical atherosclerosis in healthy, middle-aged women. Arterioscler Thromb Vasc Biol. 1999, 19 (8): 1986-1991. 10.1161/01.ATV.19.8.1986.View ArticlePubMedGoogle Scholar
- Kim C, Newton KM, Knopp RH: Gestational diabetes and the incidence of type 2 diabetes: a systematic review. Diabetes Care. 2002, 25 (10): 1862-1868. 10.2337/diacare.25.10.1862.View ArticlePubMedGoogle Scholar
- Solano MP, Perry AC, Wang X, Ross R, Goldberg RB: Insulin resistance but not visceral adipose tissue is associated with plasminogen activator inhibitor type 1 levels in overweight and obese premenopausal African-American women. Int J Obes Relat Metab Disord. 2003, 27 (1): 82-87. 10.1038/sj.ijo.0802192.View ArticlePubMedGoogle Scholar
- Hofstee HM, Serné EH, Roberts C, Hesselstrand R, Scheja A, Moore TL, Wildt M, Manning JB, Vonk Noordegraaf A, Voskuyl AE, Herrick AL: Comment on: a multicentre study on the reliability of qualitative and quantitative nailfold videocapillaroscopy assessment: reply. Rheumatology (Oxford). 2012, 51 (4): 749-755. 10.1093/rheumatology/ker403.View ArticleGoogle Scholar
- Tooke JE: A capillary pressure disturbance in young diabetics. Diabetes. 1980, 29 (10): 815-819. 10.2337/diacare.20.10.815.View ArticlePubMedGoogle Scholar
- Ditzel J: Affinity hypoxia as a pathogenetic factor of microangiopathy with particular reference to diabetic retinopathy. Acta Endocrinol Suppl (Copenh). 1980, 238: 39-55.Google Scholar
- Anastasiou E, Lekakis JP, Alevizaki M, Papamichael CM, Megas J, Souvatzoglou A, Stamatelopoulos SF: Impaired endothelium-dependent vasodilatation in women with previous gestational diabetes. Diabetes Care. 1998, 21 (12): 2111-2115. 10.2337/diacare.21.12.2111.View ArticlePubMedGoogle Scholar
- Hannemann MM, Liddell WG, Shore AC, Clark PM, Tooke JE: Vascular function in women with previous gestational diabetes mellitus. J Vasc Res. 2002, 39 (4): 311-319. 10.1159/000065543.View ArticlePubMedGoogle Scholar
- Hu J, Norman M, Wallensteen M, Gennser G: Increased large arterial stiffness and impaired acetylcholine induced skin vasodilatation in women with previous gestational diabetes mellitus. Br J Obstet Gynaecol. 1998, 105 (12): 1279-1287. 10.1111/j.1471-0528.1998.tb10006.x.View ArticlePubMedGoogle Scholar
- Banerjee M, Anderson SG, Malik RA, Austin CE, Cruickshank JK: Small artery function 2 years postpartum in women with altered glycaemic distributions in their preceding pregnancy. Clin Sci (Lond). 2012, 122 (2): 53-61. 10.1042/CS20110033.View ArticleGoogle Scholar
- Caballero AE, Arora S, Saouaf R, Lim SC, Smakowski P, Park JY, King GL, LoGerfo FW, Horton ES, Veves A: Microvascular and macrovascular reactivity is reduced in subjects at risk for type 2 diabetes. Diabetes. 1999, 48 (9): 1856-1862. 10.2337/diabetes.48.9.1856.View ArticlePubMedGoogle Scholar
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