- Open Access
Prevalence of adrenal masses in Japanese patients with type 2 diabetes mellitus
© Hiroi et al; licensee BioMed Central Ltd. 2010
- Received: 22 June 2010
- Accepted: 20 December 2010
- Published: 20 December 2010
To date, there have been no reports on the prevalence of adrenal masses in type 2 diabetic patients. The present study aimed to evaluate the prevalence of adrenal incidentaloma in type 2 diabetic patients in Japan.
We retrospectively evaluated the presence of adrenal masses using abdominal CT scans in 304 type 2 diabetic patients. In those with adrenal masses, we examined the hormone production capacity of the adrenal mass.
Fourteen patients (4.6%) had an adrenal mass. Hormonal analysis identified one case as having subclinical Cushing's syndrome, two with primary aldosteronism. Eleven cases had non-functioning masses.
The reported prevalence of adrenal incidentaloma in normal subjects is 0.6-4.0% in abdominal CT scan series. Our results show a relatively high prevalence of adrenal tumors in diabetic patients. On the other hand, the frequency of functional adenoma in diabetic patients is 21.4%, which is similar to that of normal subjects.
Although further studies are needed to evaluate the prevalence of adrenal tumors in diabetic patients, our data suggest that evaluation of the presence of adrenal masses may be needed in patients with type 2 diabetes mellitus.
- Diabetic Patient
- Primary Aldosteronism
- Adrenal Tumor
- Adrenal Mass
- Adrenal Adenoma
The detection of clinically silent adrenal masses, known as adrenal incidentalomas, has increased. This has resulted from both technical improvements in abdominal imaging devices and their increased usage. Typically, these techniques include ultrasonography (US), computed tomography (CT scan), and magnetic resonance imaging . As a result, adrenal incidentalomas have become a relatively common finding in clinical practice [2–4]. Many patients with adrenal incidentalomas display altered glucose tolerance, insulin resistance and metabolic syndrome [5, 6], though there are no reports on the frequency of adrenal masses in type 2 diabetic patients in Japan. Therefore, we investigated the presence of adrenal tumors in Japanese type 2 diabetic patients.
General data for the 304 patients with type 2 diabetes mellitus
Diabetic patients (n = 304)
without adrenal mass (n = 290)
with adrenal mass (n = 14)
62.1 ± 13.1
58.7 ± 14.0
160.7 ± 9.6
162.3 ± 11.6
64.8 ± 16.5
72.7 ± 21.7
Body mass index (kg/m2)
24.9 ± 5.6
27.5 ± 7.1
Blood Glucose (mg/dL)
221.1 ± 137.5
168.2 ± 59.8
Glycosylated Hemoglobin (%)
8.5 ± 6.6
7.7 ± 1.4
Urinary C-peptide (μg/day)
46.3 ± 41.2
41.3 ± 15.2
Urinary albumin excretion (mg/g·Cr)
317.7 ± 870.0
437.4 ± 1256.7
Known duration of diabetes (years)
10.3 ± 9.9
12.1 ± 12.1
Abdominal CT scan and US were performed to screen for tumorous lesions including malignant disorders at the time that these diabetic patients presented to our outpatient clinics. Only abdominal CT scan data were selected for this study, because the resolution of CT scans is better than that of US. The imaging characteristics used for the detection of adrenal masses were recorded on the basis of both of a radiologist's and an endocrinologist's description. The diagnosis of cortical adenoma was based on the following CT scan criteria: size >10 mm, smooth margin and homogeneous with a relatively low CT density (≤10 Hounsfield units [HU] on unenhanced CT scan). A non-contrast CT scan attenuation value of 10 HU is a safe cut-off value for differentiating adrenal adenomas from non-adenomas [7, 8].
The following hormonal determinations were performed in patients with adrenal masses in order to diagnose the presence of functional adenomas: levels of plasma adrenocorticotropic hormone (ACTH), and serum cortisol, aldosterone, dehydroepiandrosterone-sulfate (DHEA-S) and catecholamines, and plasma renin activity (PRA). Serum cortisol levels were evaluated after an overnight 1 or 8 mg dexamethasone suppression test for the diagnosis of subclinical Cushing's syndrome (SCS). When serum cortisol remained above 3.0 μg/dL using 1 mg dexamethasone or 1 μg/dL using 8 mg dexamethasone, this indicated insufficient suppression. This criterion is recommended by the 'Disorders of Adrenal Hormones' Research Committee of the Japanese Ministry of Health and Welfare . The diagnosis of SCS was made according to the criteria established in the same report . Primary aldosteronism (PA) was diagnosed using the 40 mg furosemide-upright test and the 50 mg captopril-loading test in patients with an aldosterone-renin ratio (ARR) of more than 200. Patients with a PRA level below 1.0 ng/mL/hr 120 minutes after furosemide injection and ARR of more than 300 at 90 minutes after captopril administration were diagnosed as having PA [10–12].
Samples used for measuring hormone levels were all collected at 9 am and centrifuged at 4°C, and plasma and serum were then separated and stored at -20°C until the assays. Plasma ACTH was measured using a commercially available ECLIA kit (Roche Diagnostics, Tokyo, Japan). Serum cortisol and DHEA-S (Diagnostic Products Corp., Tokyo, Japan), and aldosterone (Dinabot Corp., Osaka, Japan) were measured with commercially available RIA kits. Plasma renin activity was determined by RIA kit (TFB, INC., Tokyo, Japan). Catecholamines, adrenaline and noradrenaline were measured by the specific HPLC method .
Measurements for diabetic patients with and without adrenal masses were compared using Student's t test, and P < 0.05 were regarded as significant. Data are expressed as means ± SD.
An analysis of the age and gender distribution of diabetic patients with adrenal masses showed that they do not differ from diabetic patients without such masses. We determined that there were no significant differences in height, weight, body mass index and known duration of diabetes between these groups. Nor was any significant difference observed in diabetic parameters, such as fasting blood glucose, glycosylated hemoglobin, urinary C-peptide and urinary albumin excretion (Table 1).
Characteristics for the 14 patients with adrenal lesions
Confirm exam for PA
7.2 ~ 63.3
4.0 ~ 18.3
29.9 ~ 159
0.3 ~ 2.9
Mean ± SD
33.6 ± 22.5
14.9 ± 6.4
2.4 ± 1.1
89.9 ± 45.6
1.1 ± 0.5
16.3 ± 5.6
7.3 ± 2.9
136.6 ± 6.4
82.4 ± 4.7
The levels of plasma ACTH (33.6 ± 22.5 pg/mL) and serum cortisol (14.9 ± 6.4 μg/dL) in patients with adrenal masses were within normal ranges. Serum cortisol levels were suppressed after the administration of 1 mg of dexamethasone in cases 1 to 13 but not in case 14 with either 1 mg or 8 mg dexamethasone. The DHEA-S levels were within the age-matched normal range in all but case 14. The aldosterone and PRA levels were 89.9 ± 45.6 pg/mL and 1.1 ± 0.5 ng/mL/hr, respectively. In cases 12 and 13, the ARR was >200, and the furosemide-upright test and captopril loading test were positive but in all other cases the ARR values were <200. We thus diagnosed cases 12 and 13 as having PA, case 14 as having SCS only, and the other 11 cases as having non-functioning adrenal tumors. In both patients with PA, normalization of the ARR and blood pressure decreases were observed after adrenalectomy. However, the SCS case was not treated surgically, because this patient refused adrenalectomy. Since adrenaline and noradrenaline levels were within normal range (the total of adrenaline plus noradrenaline was <500 pg/mL), the possibility of pheochromocytoma was excluded (data not shown).
The mean prevalence of adrenal incidentalomas as determined in autopsy series is 1.9-8.7% [14–16], whereas in CT scan series published in the period from 1982 to 1994, it was 0.6-1.5% [14, 15, 17–19]. Recently, Bovio et al reported that the prevalence of adrenal masses on abdominal CT scans was 4.4% in Italian patients with high risk of lung cancer, and that of benign adrenal masses was 4.2% . In our study, we observed a relatively high prevalence of adrenal masses (4.6%) in Japanese type 2 diabetic patients. Some reports have shown that patients with adrenal incidentaloma have insulin resistance and metabolic syndrome, although the majority of these masses do not show hormonal hyper-secretion [5, 6, 21–23]. Terzolo et al demonstrated that non-functioning adenomas have different patterns of steroid secretion , and subtle autonomous cortisol secretion might be one of the reasons for the high prevalence of adrenal incidentaloma in type 2 diabetic patients. In addition, recent advances in CT scan technology could be expected to lead to an increased rate of adrenal incidentaloma discovery.
In approximately 70% of cases, adrenal incidentalomas are non-functional adenomas . Investigations of adrenal incidentalomas in Japan have shown the prevalence of functional adrenal adenoma to be approximately 50% . The frequencies of cortisol-producing adenoma and PA in adrenal incidentaloma in Asian populations are 6.0-11.7% and 3.0-4.3%, respectively [25, 26]. Herein, a functional adrenal mass in type 2 diabetic patients with adrenal incidentalomas was seen in three out of 14 cases (21.4%), with one case of SCS (7.1%) and two of PA (14.3%). It should be noted that the small number of functional tumors identified in this report may have limited the identification of other types of adrenal functional tumors.
The prevalence of subclinical hyper-cortisolism of adrenal origin in diabetic patients is reported to be 1.1-7.1% [27–29]. In the present study, the prevalence of adrenal SCS was only 0.7% in type 2 diabetic patients. This may be one reason for the low prevalence of adrenal SCS, i.e., that the Japanese diagnostic criteria for SCS were used in this study. The cut-off value of the 1 mg dexamethasone suppression test in the Japanese diagnostic criteria was high, 3.0 μg/dL, in comparison with the diagnostic criteria of the Endocrine Society, for which the cut-off value after 1 mg dexamethasone administration is 1.8 μg/dL . Although levels of cortisol exceeded 1.8 μg/dL after administration of 1 mg dexamethasone in cases 2, 5, 6, 7, 10 and 11, DHEA-S levels were normal. Therefore, the possibility of diabetes seems low in these cases. On the other hand, in case 14 diagnosed with SCS, the DHEA-S level was suppressed with 8.0 μg/dL.
To our knowledge, there are no reports indicating that diabetes induces adrenal incidentaloma, SCS or PA. On the other hand, the pathogenesis of diabetes mellitus is reportedly associated with PA related to impaired insulin secretion due to hypokalemia . Colussi et al demonstrated a direct relationship between aldosterone, insulin resistance and hyperinsulinemia [32, 33]. Overproduction of cortisol is reportedly associated with insulin resistance , and higher cortisol concentrations are also related to reduced insulin secretion . Therefore, in patients with SCS and PA, diabetes mellitus may develop.
In this study, adrenal masses were found at the same frequency on the right (50.0%) and left sides (42.9%), whereas bilateral masses were detected in one (7.1%) of our cases. A similar lateral distribution of adrenal masses has been reported in CT scan series [25, 36, 37]. An evaluation of the lateral distribution of adrenal masses using abdominal US showed right predominance , whereas Bovio et al reported that masses were detected more frequently in the left adrenal gland .
Previously, the sole predictor of the risk of adrenocortical carcinoma was mass size [25, 36]. The incidence of malignant adrenal tumors is significantly higher in cases where the size of the masses is greater than 40-50 mm [24, 25]. The mean diameter of adrenal masses discovered on CT scans was 16.3 ± 5.6 mm, ranging from 10.0 to 26.0 mm, with no malignancy observed in any of our cases. In addition, these data confirm previous results and show the mean size of functional masses to be significantly higher than that of non-functioning masses [37–39].
The frequency of functional adrenal masses in Japanese type 2 diabetic patients with such masses was almost the same as that in normal subjects, though a relatively high prevalence of adrenal masses was found in our study. Although further studies are needed to evaluate the frequency of adrenal masses in Japanese type 2 diabetic patients, our data suggest that evaluation for the presence of adrenal masses may be needed in patients with type 2 diabetes mellitus.
- Brunt LM, Moley JF: Adrenal incidentaloma. World J Surg. 2001, 25: 905-13. 10.1007/s00268-001-0029-0.View ArticlePubMedGoogle Scholar
- Kloos RT, Gross MD, Francis IR, Korobkin M, Shapiro B: Incidentally discovered adrenal masses. Endocr Rev. 1995, 16: 460-484.PubMedGoogle Scholar
- Grumbach MM, Biller BM, Braunstein GD, Campbell KK, Carney JA, Godley PA, Harris EL, Lee JK, Oertel YC, Posner MC, Schlechte JA, Wieand HS: Management of the clinically inapparent adrenal mass ("incidentaloma"). Ann Intern Med. 2003, 138: 424-429.View ArticlePubMedGoogle Scholar
- Mansmann G, Lau J, Balk E, Rothberg M, Miyachi Y, Bornstein SR: The Clinically Inapparent Adrenal Mass: Update in Diagnosis and Management. Endocr Rev. 2004, 25: 309-40. 10.1210/er.2002-0031.View ArticlePubMedGoogle Scholar
- Midorikawa S, Sanada H, Hashimoto S, Suzuki T, Watanabe T: The improvement of insulin resistance in patients with adrenal incidentaloma by surgical resection. Clin Endocrinol (Oxf). 2001, 54: 797-804. 10.1046/j.1365-2265.2001.01274.x.View ArticleGoogle Scholar
- Reincke M, Fassnacht M, Väth S, Mora P, Allolio B: Adrenal incidentalomas: a manifestation of the metabolic syndrome?. Endocr Res. 1996, 22: 757-61.PubMedGoogle Scholar
- Gross MD, Shapiro B: Clinically silent adrenal masses. J Clin Endocrinol Metab. 1993, 77: 885-888. 10.1210/jc.77.4.885.PubMedGoogle Scholar
- Hamrahian AH, Ioachimescu AG, Remer EM, Motta-Ramirez G, Bogabathina H, Levin HS, Reddy S, Gill IS, Siperstein A, Bravo EL: Clinical Utility of Noncontrast Computed Tomography Attenuation Value (Hounsfield Units) to Differentiate Adrenal Adenomas/Hyperplasias from Nonadenomas: Cleveland Clinic Experience. J Clin Endocrinol Metab. 2005, 90: 871-877. 10.1210/jc.2004-1627.View ArticlePubMedGoogle Scholar
- Nawata H, Demura H, Suda T, Takayanagi R: Adrenal preclinical Cushing's syndrome. Annual report of the Ministry of Health and Welfare 'Disorder of Adrenal Hormones' Research Committee, Japan. 1996, 223-226. (In Japanese)Google Scholar
- Omura M, Saito J, Yamaguchi K, Kakuta Y, Nishikawa T: Prospective study on the prevalence of secondary hypertension among hypertensive patients visiting a general outpatient clinic in Japan. Hypertens Res. 2004, 27: 193-202. 10.1291/hypres.27.193.View ArticlePubMedGoogle Scholar
- Nishikawa T, Saito J, Omura M: Prevalence of primary aldosteronism: should we screen for primary aldosteronism before treating hypertensive patients with medication?. Endocr J. 2007, 54: 487-495. 10.1507/endocrj.KR-105.View ArticlePubMedGoogle Scholar
- Arlt W: A detour guide to the Endocrine Society Clinical Practice Guideline on case detection, diagnosis and treatment of patients with primary aldosteronism. Eur J Endocrinol. 2010, 162: 435-438. 10.1530/EJE-09-0869.View ArticlePubMedGoogle Scholar
- Nohta H, Lee MK, Ohkura Y: Fluorescence products of the reaction for the determination of catecholamines with 1,2-diarylethylendiamines. Analytica Chimica Acta. 1992, 267: 137-139. 10.1016/0003-2670(92)85015-X.View ArticleGoogle Scholar
- Meagher AP, Hugh TB, Casey JH, Chisholm DJ, Farrell JC, Yeates M: Primary adrenal tumours---a ten-year experience. Aust N Z J Surg. 1988, 58: 457-462. 10.1111/j.1445-2197.1988.tb06235.x.View ArticlePubMedGoogle Scholar
- Abecassis M, McLoughlin MJ, Langer B, Kudlow JE: Serendipitous adrenal masses: prevalence, significance, and management. Am J Surg. 1985, 149: 783-788. 10.1016/S0002-9610(85)80186-0.View ArticlePubMedGoogle Scholar
- Hedeland H, Ostberg G, Hokfelt B: On the prevalence of adrenocortical adenomas in an autopsy material in relation to hypertension and diabetes. Acta Med Scand. 1968, 184: 211-214. 10.1111/j.0954-6820.1968.tb02445.x.View ArticlePubMedGoogle Scholar
- Glazer HS, Weyman PJ, Sagel SS, Levitt RG, McClennan BL: Nonfunctioning adrenal masses: incidental discovery on computed tomography. AJR Am J Roentgenol. 1982, 139: 81-85.View ArticlePubMedGoogle Scholar
- Caplan RH, Strutt PJ, Wickus GG: Subclinical hormone secretion by incidentally discovered adrenal masses. Arch Surg. 1994, 129: 291-296.View ArticlePubMedGoogle Scholar
- Prinz RA, Brooks MH, Churchill R, Graner JL, Lawrence AM, Paloyan E, Sparagana M: Incidental asymptomatic adrenal masses detected by computed tomographic scanning. Is operation required?. JAMA. 1982, 248: 701-704. 10.1001/jama.248.6.701.View ArticlePubMedGoogle Scholar
- Bovio S, Cataldi A, Reimondo G, Sperone P, Novello S, Berruti A, Borasio P, Fava C, Dogliotti L, Scagliotti GV, Angeli A, Terzolo M: Prevalence of adrenal incidentaloma in a contemporary computerized tomography series. J Endocrinol Invest. 2006, 29: 298-302.View ArticlePubMedGoogle Scholar
- Terzolo M, Osella G, Alì A, Borretta G, Magro GP, Termine A, Paccotti P, Angeli A: Different patterns of steroid secretion in patients with adrenal incidentaloma. J Clin Endocrinol Metab. 1996, 81: 740-744. 10.1210/jc.81.2.740.PubMedGoogle Scholar
- Fernandez-Real JM, Ricart EW, Simò R, Salinas I, Webb SM: Study of glucose tolerance in consecutive patients harbouring incidental adrenal tumours. Clin Endocrinol (Oxf). 1998, 49: 53-61. 10.1046/j.1365-2265.1998.00437.x.View ArticleGoogle Scholar
- Muscogiuri G, Sorice GP, Prioletta A, Mezza T, Cipolla C, Salomone E, Giaccari A, Pontecorvi A, Casa SD: The size of adrenal incidentalomas correlates with insulin resistance. Is there a cause-effect relationship?. Clin Endocrinol (Oxf). 2010,Google Scholar
- Barzon L, Sonino N, Fallo F, Palu G, Boscaro M: Prevalence and natural history of adrenal incidentalomas. Eur J Endocrinol. 2003, 149 (4): 273-85. 10.1530/eje.0.1490273.View ArticlePubMedGoogle Scholar
- Ueshiba H, Ichijo T, Yamamoto N: A survey of adrenal incidentaloma in Japan. Annual report of the Health and Labour Science Research Grant 'Disorder of Adrenal Hormones' Research Committee, Japan. 2003, 117-119. (In Japanese)Google Scholar
- Kim HY, Kim SG, Lee KW, Seo JA, Kim NH, Choi KM, Baik SH, Choi DS: Clinical study of adrenal incidentaloma in Korea. Korean J Intern Med. 2005, 20: 303-309.PubMed CentralView ArticlePubMedGoogle Scholar
- Leibowitz G, Tsur A, Chayen SD, Salameh M, Raz I, Cerasi E, Gross DJ: Pre-clinical Cushing's syndrome: an unexpected frequent cause of poor glycaemic control in obese diabetic patients. Clin Endocrinol (Oxf). 1996, 44: 717-722. 10.1046/j.1365-2265.1996.737558.x.View ArticleGoogle Scholar
- Catargi B, Rigalleau V, Poussin A, Ronci-Chaix N, Bex V, Vergnot V, Gin H, Roger P, Tabarin A: Occult Cushing's syndrome in type-2 diabetes. J Clin Endocrinol Metab. 2003, 88: 5808-5813. 10.1210/jc.2003-030254.View ArticlePubMedGoogle Scholar
- Chiodini I, Torlontano M, Scillitani A, Arosio M, Bacci S, Di Lembo S, Epaminonda P, Augello G, Enrini R, Ambrosi B, Adda G, Trischitta V: Association of subclinical hypercortisolism with type 2 diabetes mellitus: a case-control study in hospitalized patients. Eur J Endocrinol. 2005, 153 (6): 837-44. 10.1530/eje.1.02045.View ArticlePubMedGoogle Scholar
- Nieman LK, Biller BM, Findling JW, Newell-Price J, Savage MO, Stewart PM, Montori VM: The Diagnosis of Cushing's Syndrome: An Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab. 2008, 93: 1526-1540. 10.1210/jc.2008-0125.PubMed CentralView ArticlePubMedGoogle Scholar
- Rowe JW, Tobin JD, Rosa RM, Andres R: Effect of experimental potassium deficiency on glucose and insulin metabolism. Metabolism. 1980, 29: 498-502. 10.1016/0026-0495(80)90074-8.View ArticlePubMedGoogle Scholar
- Catena C, Lapenna R, Baroselli S, Nadalini E, Colussi GL, Novello M, Favret G, Melis A, Cavarape A, Sechi LA: Insulin sensitivity in patients with primary aldosteronism: a follow-up study. J Clin Endocrinol Metab. 2006, 91: 3457-3463. 10.1210/jc.2006-0736.View ArticlePubMedGoogle Scholar
- Colussi G, Catena C, Lapenna R, Nadalini E, Chiuch A, Sechi LA: Insulin resistance and hyperinsulinemia are related to plasma aldosterone levels in hypertensive patients. Diabetes Care. 2007, 30: 2349-2354. 10.2337/dc07-0525.View ArticlePubMedGoogle Scholar
- Ward AM, Fall CH, Stein CE, Kumaran K, Veena SR, Wood PJ, Syddall HE, Phillips DI: Cortisol and the metabolic syndrome in South Asians. Clin Endocrinol (Oxf). 2003, 58: 500-505. 10.1046/j.1365-2265.2003.01750.x.View ArticleGoogle Scholar
- Delaunay F, Khan A, Cintra A, Davani B, Ling ZC, Andersson A, Ostenson CG, Gustafsson J, Efendic S, Okret S: Pancreatic β-cells are important targets for the diabetogenic effects of glucocorticoids. J Clin Invest. 1997, 100: 2094-2098. 10.1172/JCI119743.PubMed CentralView ArticlePubMedGoogle Scholar
- Mantero F, Terzolo M, Arnaldi G, Osella G, Masini AM, Alì A, Giovagnetti M, Opocher G, Angeli A: A survey on adrenal incidentaloma in Italy. Study Group on Adrenal Tumors of the Italian Society of Endocrinology. J Clin Endocrinol Metab. 2000, 85: 637-644. 10.1210/jc.85.2.637.PubMedGoogle Scholar
- Herrera MF, Grant CS, van Heerden JA, Sheedy PF, Ilstrup DM: Incidentally discovered adrenal tumors: an institutional perspective. Surgery. 1991, 110: 1014-1021.PubMedGoogle Scholar
- Barzon L, Fallo F, Sonino N, Boscaro M: Development of overt Cushing's syndrome in patients with adrenal incidentaloma. Eur J Endocrinol. 2002, 146: 61-66. 10.1530/eje.0.1460061.View ArticlePubMedGoogle Scholar
- Reincke M, Nieke J, Krestin GP, Saeger W, Allolio B, Winkelmann W: Preclinical Cushing's syndrome in adrenal "incidentalomas": comparison with adrenal Cushing's syndrome. J Clin Endocrinol Metab. 1992, 75: 826-832. 10.1210/jc.75.3.826.PubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.