Fig. 7

Sacubitril/valsartan attenuated tubulointerstitial fibrosis through Sirt1-mediated mitochondrial dysfunction. (A) Urinary NAG. (B) Serum creatinine. (C) blood urea nitrogen. (D) Urinary albumin-to-creatinine. (E) PAS staining, representative micrographs were shown. (F) Tubular injury score in PAS-stained sections. (G) Masson staining, representative micrographs were shown. (H) The percentage of tubulointerstitial fibrotic area in Masson trichrome-stained sections. (I-K) Representative Western blotting images and densitometric analysis of collagen 1 and α-SMA in HK-2 cells. Scale bar = 50 μm. Results represent means ± SEM, n = 4. *P < 0.05, **P < 0.01, ***P < 0.001. Sac/Val, DKD + Sacubitril/Valsartan treated; Sac/Val + EX527, DKD + Sacubitril/Valsartan and EX527 treated; NC, normal control; HM, high mannitol; HG, high glucose; HG + Val, high glucose + Valsartan; HG + LBQ/Val, high glucose + LBQ657 + valsartan