Skip to main content
Fig. 6 | Diabetology & Metabolic Syndrome

Fig. 6

From: Sacubitril/valsartan ameliorates tubulointerstitial fibrosis by restoring mitochondrial homeostasis in diabetic kidney disease

Fig. 6

The improvement of mitochondrial function by in Vitro sacubitril (LBQ657) /valsartan was attenuated by Sirt1 inhibition in the HG-treated HK-2 cells. (A) Representative western blotting images and densitometric analysis of Sirt1 and PGC1α of HK-2 cells from HG + LBQ/Val and HG + LBQ/Val + EX527 group. (B) Representative images and statistical graphs of immunofluorescent staining for Sirt1. (C) Representative Western blotting images and densitometric analysis of Tfam and Cox IV;. (D) Mitochondrial membrane potential assessed by JC-1 assay. (E) Typical fluorescence photomicrograph and quantitative analysis of mitochondrial superoxide. (F) Representative images of mitochondria morphology with MitoTracker staining and quantitative analysis of the percentage of cells with mitochondrial fragmentation. Results represent means ± SEM, n = 4. *P < 0.05, **P < 0.01, ***P < 0.001. HG, high glucose; Val, valsartan; LBQ/Val, LBQ657 + valsartan

Back to article page

Diabetology & Metabolic Syndrome

ISSN: 1758-5996

Contact us