Fig. 3

Sacubitril/valsartan improved mitochondrial morphology and mitochondrial biogenesis. (A-C) Functions of differential expressed genes between DKD group and Sac/Val group (D) Representative transmission electron microscopy of mitochondria in tubular cells of mice. (E) Aspect ratio of mitochondria in tubular epithelial cells. (F) Form factor of mitochondria in tubular epithelial cells. (G, H) Representative western blotting images and densitometric analysis of Tfam and Cox IV; in kidney from mice in different groups. (I) Representative images and statistical graphs of immunohistochemical staining of Tfam in kidney sections. (J, K) Representative western blotting images and densitometric analysis of Tfam and Cox IV; in HK-2 cells. Scale bar = 50 μm. Results represent means ± SEM, n = 4. *P < 0.05; **P < 0.01; ***P < 0.001 DKD, diabetic kidney disease; Val, DKD + Valsartan treated; Sac/Val, DKD + Sacubitril/Valsartan treated; NC, normal control; HM, high mannitol; HG, high glucose; HG + Val, high glucose + Valsartan; HG + LBQ/Val, high glucose + LBQ657 + valsartan