Fig. 2

Sacubitril/valsartan attenuated tubulointerstitial fibrosis in diabetic mice. (A) PAS staining, representative micrographs were shown. (B) Tubular injury score in PAS-stained sections. (C) Masson staining, representative micrographs were shown. (D) The percentage of tubulointerstitial fibrotic area in Masson trichrome-stained sections. (E, F) Representative western blotting images and densitometric analysis of KIM-1. (E, G) Representative western blotting images and densitometric analysis of Collagen 1 and α-SMA. Scale bar = 50 μm. Results represent means ± SEM, n = 4. *P < 0.05, **P < 0.01, ***P < 0.001. DKD, diabetic kidney disease; Val, DKD + Valsartan treated; Sac/Val, DKD + Sacubitril/Valsartan treated; PAS, periodic acid-Schiff base