Fig. 4
Dynamics of the polarized phenotype of microglia. A Pseudotime trajectory analysis showing the polarization characteristics of the three groups of microglia. Different colors represent different microglial subtypes. The arrows point to the start and end points of the trajectory. B Ridge plot showing the distribution of three groups of microglia in pseudotime. C Heatmap (right) showing changes in gene expression during pseudotime. Genes in the trajectory are divided into 5 modules. The KEGG analysis result (adjusted p < 0.05) corresponding to each module is on the left. D Feature plot showing the expression changes in selected genes over pseudotime. E Significant genes in the pseudotime analysis, including surface proteins, transcription factors (TFs), and all genes. F Ridge plot of the significant pathway (GO) terms over pseudotime. FDR < 0.05. G Gene expression of inflammatory receptors over pseudotime. The different colors correspond to different cell types