Fig. 5

RYGB actives AMPK to upregulate GLUT4 in db/db mice. A and B, Representative western blots and quantitative results of GLUT4, AMPK, pAMPK, STAT3, pSTAT3, pAKT and AKT in the liver from db/m mice and db/db mice after sham and RYGB surgery (n = 4). Protein levels were normalized to those of β-actin. C and D, Representative western blots and quantitative results of GLUT4, AMPK, pAMPK, STAT3, pSTAT3, pAKT and AKT in the sWAT from db/m mice and db/db mice after sham and RYGB surgery (n = 4). Protein levels were normalized to those of β-actin. sWAT, subcutaneous white adipose tissue. E and F, Representative western blots and quantitative results of GLUT4, AMPK, pAMPK, STAT3, pSTAT3, pAKT and AKT in the eWAT from db/m mice and db/db mice after sham and RYGB surgery (n = 4). Protein levels were normalized to those of β-actin. eWAT, epididymal white adipose tissue. G, Representative PET/CT images (left) and the relative quantification of [18 F]-FDG uptake (right) from db/m mice and db/db mice after sham and RYGB surgery (n = 6). In the PET/CT image, the yellow arrow points towards the liver, with its cross-section at the bottom. The data are presented as the mean ± SEM. *P < 0.05, **P < 0.01***P < 0.001, ****P < 0.0001, compared with the db/m sham group; #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.001, compared with the db/db sham group, by one-way ANOVA