Fig. 4

The changes of glucose and lipid metabolism in the liver and white adipose. A and B, Representative western blots and quantitative results of GCK, PFKL, PKLR, ACLY and CPT1A in the liver from db/m mice and db/db mice after sham and RYGB surgery (n = 4). Protein levels were normalized to those of β-actin. C and D, Representative western blots and quantitative results of FASN, SREBP1 and CPT1A in the sWAT from db/m mice and db/db mice after sham and RYGB surgery (n = 4). Protein levels were normalized to those of β-actin. sWAT, subcutaneous white adipose tissue. E and F, Representative western blots and quantitative results of FASN, SREBP1 and CPT1A in the eWAT from db/m mice and db/db mice after sham and RYGB surgery (n = 4). Protein levels were normalized to those of β-actin. eWAT, epididymal white adipose tissue. G and H, RER and EE values of db/m and db/db mice after sham and RYGB surgery (n = 4–6). Quantitative results are shown on the right. The data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, compared with the db/m sham group; #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.001, compared with the db/db sham group, by one-way ANOVA