Fig. 3

Effect of RYGB on fat distribution in db/db mice after RYGB. A, Representative images of fasting and feeding liver morphology and Oil red staining in db/db mice after sham and RYGB surgery. Scale bar, 50 μm. The quantitative results of the relative area of lipid droplets are shown on their right (n = 5). B, schematic diagram of micro-CT in RYGB-treated db/db mice. C, The subcutaneous and visceral fat volume and fat percent in db/db mice after sham and RYGB surgery (n = 4). D, Blood lipid levels in db/m mice and db/db mice after sham and RYGB surgery (n = 4). E and F, Representative images of H&E staining and quantitative results of lipid droplet number of BAT, iWAT, PAT, mWAT, and eWAT of db/m mice and db/db mice after sham and RYGB surgery (n = 5). Scale bar, 50 μm. G, The blood adiponectin in db/m mice and db/db mice after sham and RYGB surgery (n = 8). The data are presented as the mean ± SEM. *p < 0.05, **p<0.01,***p < 0.001, ****p < 0.0001. SAT, subcutaneous adipose tissue; VAT, visceral adipose tissue; TC, total cholesterol; TG, triglycerides; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density lipoprotein cholesterol; BAT, brown adipose tissue; iWAT, inguinal white adipose tissue; PAT, perirenal adipose tissue; mWAT, mesenteric white adipose tissue; eWAT, epididymis white adipose tissue