Fig. 7

EGFR overexpression promotes the proliferation and migration of HaCaT cells in high-glucose environments. (A) RT-qPCR was used to determine the relative EGFR expression levels. (B) The relative EGFR expression levels were determined using Western blotting. (C) Inverted fluorescence microscopy was used to observe the cell lentiviral transfection efficiency. (D) The relative EGFR expression levels were determined using RT-qPCR. (E) The relative EGFR expression was estimated using Western blotting. (F) Cell proliferative ability measured using CCK-8 assays. (G) Migrated cells were counted and analysed using Transwell assays. (H) Wound healing assay and images taken at 0 and 48 h.*P < 0.05, ***P < 0.001, ****P < 0.0001