Fig. 5

SLC7A11 and miR-144-3p are targets of circ-Snhg11. (A) Genomic loci showing the Snhg11 gene and mmu_circ_0001100. (B) RT-qPCR showing the interactions between miRNAs. Data are given as mean ± SD. (C) Bioinformatics data predicting the miR-144-3p binding site on circ-Snhg11. MUT version of circ-Snhg11 is given. (D) Relative luciferase activity was determined 2 d post-HEK293T cell transfection with miR-144-3p mimic/NC or circ-Snhg11 WT/Mut. (E) miR-144-3p binding site predictions in SLC7A11 3’-UTR. MUT 3’-UTR-SLC7A11 is given. (F) Relative luciferase activity 2 d post-HEK293T cell transfection with miR-144-3p mimic/NC or 3’-UTR-SLC7A11 WT/Mut. Data are given as mean ± SD. ^*P < 0.05, ^**P < 0.01,^***P < 0.001 vs. PBS