Fig. 4

CRP deficiency enhance the role of peripheral leptin on restoring insulin sensitivity in PCOS rats fed a ND or HFD. (a) Experimental procedure and EHC protocol. (b) GIR time course (c) Cumulative GIR. (d) Hepatic glucose production (HGP). (e) Percentage of suppression of HGP. (f) Glucose disappearance rates (GRD). (g) RT-PCR assays of hepatic PEPCK mRNA (h, i) Immunoblotting assays of hepatic expression of PEPCK protein, phosphorylation of InsR and Akt and densitometric quantification. Data are expressed as the mean ± SEM (n = 5–6 rats for each group). ^*P < 0.05, ^**P < 0.01 vs. ND-DHEA-WT group; ^#P < 0.05, ^##P < 0.01 HFD-DHEA-WT group