Fig. 2

Detection of differentially expressed metabolites by pairwise comparison of T-T2DM. (A) Hierarchical clustering of differentially expressed metabolites. The median expression levels of metabolites (n = 30) for two groups are presented in the heatmap. (B) Score plots from the PCA model derived from the UPLC-MS profile of serum in two groups. (C) Score plots from the OPLS-DA model from metabolic profiles of two groups. (D) Pie-chart for the classification of significant differentially expressed metabolites (n = 412) according to meta-intensity. (E) Volcano plot of significantly differentially expressed metabolites with marking the top 5 up and down expression differential metabolites (Red represents up-regulated, blue represents down-regulated metabolites). (F) Representative box plots for top up-regulated and down-regulated metabolites