Fig. 5

The effects of dapagliflozin on apoptosis and DNA fragmentation in cardiomyocytes. a Representative images of TUNEL staining. Bar = 200 μm, magnification = ×10. b Quantification of TUNEL-positive nuclei. c Representative western blot bands of cleaved PARP-1 and Bcl-2 levels in the myocardium. d, e Relative protein levels were densitometrically quantified with ImageJ. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01 vs. Lean; ^#P < 0.05, ^##P < 0.01 vs. ZDF