Fig. 4

HDB can improve muscle tissue damage and attenuate muscle lipid deposition in prediabetic model mice. (A) HE staining (400×). The skeletal muscle tissue structure of the mice in the control group was normal, the sarcolemma was intact, no obvious lesions were found, and the cell nuclei were arranged neatly under the sarcolemma. The skeletal muscle structure of the model mice was abnormal, the number of nuclei increased and was pathologically aggregated, the arrangement of muscle cells was disordered, the space between the sarcolemma increased. The histopathological structure of skeletal muscle in the HDB and metformin administration groups was improved, muscle fibers were gradually complete and regular, muscle cells were gradually arranged, skeletal muscle tissue structure was basically restored. (B) Oil red O staining (400×). (C) The CSA of muscle fibers. (D) Oil red O expression. Data are presented as mean ± SD, n = 3. ^#P < 0.05, ^##P < 0.01 vs. control group; ^*P < 0.05, ^**P < 0.01 vs. model group