Fig. 5

Knockdown of KFL7 ameliorates high glucose-induced EndMT. A–D qRT-PCR analysis of KLF7 and the EndMT transcription factors SNAIL, SLUG and TWIST in high glucose (HG)-treated HUVECs vs control. KLF7, SNAIL, SLUG and TWIST are significantly upregulated in high glucose-treated HUVECs whereas this effect is abolished upon knockdown of KLF7 in high glucose conditions. Knockdown of KLF7 also decreases the expression of KLF7, SLUG and TWIST in mannitol conditions. The data is presented as mean value; error bars represent S.E.M.; n = 4 independent biological replicates; *p < 0.05; **p < 0.01; ***p < 0.001