Fig. 2

SIRT1 is a target gene of miR-30b. A Venn diagram of target mRNAs of miR-30b predicted by the starBase, TargetScan, miRmap, miRDB and PicTar databases. B An interaction network of candidate gene encoding proteins. The color of the circle from dark blue to light blue indicates that the Degree value of the gene is from large to small, and the line from dark gray to light gray indicates that the combined score between genes is from large to small. C Putative miR-30b binding sites in the 3′UTR of SIRT1 predicted by the starBase database. D mRNA expression of SIRT1 determined by RT-qPCR in the retinal tissues of control (n = 6) and PDR mice (n = 6). E Western blot analysis of SIRT1 protein in the retinal tissues of control (n = 6) and PDR mice (n = 6). F Expression of miR-30b and SIRT1 in NG- and HG-treated RMECs. NG normal glucose, HG high glucose. G Western blot analysis of SIRT1 protein in NG- and HG-treated RMECs. H Binding of miR-30b to SIRT1 confirmed by dual luciferase reporter assay in HEK293T cells. I Binding of miR-30b to SIRT1 confirmed by dual luciferase reporter assay in RMECs. J Expression of miR-30b and SIRT1 determined by RT-qPCR in RMECs transduced with Ad-miR-30b or In-miR-30b. K Western blot analysis of SIRT1 protein in RMECs transduced with Ad-miR-30b or In-miR-30b. *p < 0.05, compared with the control mice or NG-treated cells, HEK293T cells and RMECs transfected with NC mimic, or RMECs transduced with Ad-NC. ^#p < 0.05, compared with RMECs transduced with In-NC. Cell experiments were conducted 3 times independently