Fig. 2

High glucose treatment impacts on the biological behaviors of H9c2 cells. H9c2 cells were treated with mannitol or high glucose. A β-galactosidase staining was used to reveal cell senescence. B Flow cytometry was used to analyze cell apoptosis. C The colorimetric method was used to detect the activity of caspase-3 in cells. D DCFH-DA probes were used to detect ROS in cells. E Western blotting was used to detect Bax and Bcl-2 in cells. The data were all measurement data and expressed as mean ± standard deviation. One-way analysis of variance was used for comparisons between multiple groups and Tukey's test for post-hoc multiple comparisons. ^* indicates P < 0.05. The experiments were repeated thrice. OS osmotic, HG high glucose, DCFH-DA, 2′,7′-dichlorodihydrofluorescein diacetate, ROS reactive oxygen species, Bax Bcl-2-associated X protein, Bcl-2 B-cell lymphoma-2