Fig. 2
From: Effect of red beetroot juice on oxidative status and islet insulin release in adult male rats
GSIS assay on isolated islets and the effect of nitric oxide inhibition on islet insulin secretion from experimental groups. Insulin release was measured during 1 h from groups of 5 islets at increasing glucose concentrations (5.6–16.8 mmol/L) after overnight fasting. Nitric oxide production was inhibited by Aminoguanidine (AG). Isolated islets were incubated in vitro with AG and 8.3 mM glucose followed by stimulation with 16.7 mM glucose. Insulin release was measured during 1 h from groups of 5 islets after overnight fasting. Values are mean ± SEM for 12 cups (3 cups each containing 5 islets for each condition from each animal; 4 animals in each group). Statistical comparison between groups in each glucose concentration was made using t-test. Beetroot (red beetroot juice) vs. without AG in same group (*P < 0.05, ***P < 0.001), Beetroot (red beetroot juice) vs. control (#P < 0.05)