Fig. 1

Expression and location of FSHR in mouse kidney and JG cells. A Confocal images of kidney sections stained with FSHR (red), renin (green), or nucleus (blue) from mouse. (×200). In negative controls PBS replaced the primary antibody. Scale bar: 30 μm. B Immunofluorescent staining of FSHR in the As4.1 cells. (×400). In negative controls PBS replaced the primary antibody. Scale bar: 10 μm. C RT-PCR analysis shows that a 381-bp band representing FSHR mRNA was detected in mouse ovary (positive control), kidney and As4.1 cells, but not in negative control where cDNA was replaced by water. D Western blotting analysis showed that a 78-kDa band representing FSHR protein was detected in mouse ovary (positive control), kidney and As4.1 cells