Table 1 Sequences of specific primers for qRT-PCR

CTNNB1

Forward: 5′-ACAGGGAAGACATCACTGAGCCTGC-3′

Reverse: 5′-GGTGCATGATTTGCGGGACAAAG-3′

Wnt 1

Forward: 5′-TCCCCTTTGTCCTGCGTTTT-3′

Reverse: 5′-CTGGGAGAATGGGGGCATTT-3′

Wnt 3

Forward: 5′-AGGACAAGTATGACAGCGCC-3′

Reverse: 5′-CTCTGGGTTGGGCTCACAAA-3′

Wnt 4

Forward: 5′-ATGAGTCCCCGCTCGTG-3′

Reverse: 5′-TGGTACTGGCACTCCTCAATG-3′

Wnt5a

Forward: 5′-TCCATTCCTGGGCGCATC-3′

Reverse: 5′-CCATCCCCAAAGCAACTCCT-3′

TCF4

Forward: 5′-CCAATCACGACAGGAGGATT-3′

Reverse: 5′-CGACCTTTGCTCTCATTTCC-3′

Cyclin D1

Forward: 5′-GCTGCGAAGTGGAAACCATC-3′

Reverse: 5′-CCTCCTTCTGCACACATTTGAA-3′

CDK4

Forward: 5′-ATGGCTACCTCTCGATATGAGC-3′

Reverse: 5′-CATTGGGGACTCTCACACTCT-3′

GAPDH

Forward: 5′-CAAGGTCATCCATGACAACTTTG-3′

Reverse: 5′-GTCCACCACCCTGTTGCTGTAG-3′