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Fig. 3 | Diabetology & Metabolic Syndrome

Fig. 3

From: CircSMAD4 alleviates high glucose-induced inflammation, extracellular matrix deposition and apoptosis in mouse glomerulus mesangial cells by relieving miR-377-3p-mediated BMP7 inhibition

Fig. 3

CircSMAD4 overexpression alleviated HG-induced SV40-MES13 cell inflammation, ECM deposition and cell apoptosis. A The efficiency of circSMAD4 overexpression or knockdown was checked by qPCR. HG-treated SV40-MES13 cells were transfected with circSMAD4 or sh-circSMAD4. Then, B the expression of circSMAD4 was detected by qPCR. CF The releases of IFN-γ, MCP-1, IL-6 and TNF-α in medium were determined by ELISA. G The protein levels of fibronectin and collagen IV were measured by western blot. H Cell apoptosis was assessed by flow cytometry assay. I The protein levels of Bcl-2 and Bax were measured by western blot. *P < 0.05

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