Fig. 2
From: LncRNA KCNQ1OT1 promotes the development of diabetic nephropathy by regulating miR-93-5p/ROCK2 axis
Knockdown of KCNQ1OT1 suppressed proliferation and fibrosis and induced apoptosis in HG-treated HGMC and HRGEC. HGMC and HRGEC were transfected with si-NC or si-KCNQ1OT1 and then treated with HG. A The expression of KCNQ1OT1 was examined by qRT-PCR. B and C Cell proliferation was assessed by CCK-8 assay. D Cell apoptosis rate was analyzed through the flow cytometry analysis. E and F Western blot assay was performed to detect the protein expression of Bax and Bcl-2. G–J The protein levels of FN, Col-I, Col-IV, E-Cadherin, vimentin, and N-Cadherin were measured by western blot assay. *P < 0.05