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Fig. 3 | Diabetology & Metabolic Syndrome

Fig. 3

From: Long non-coding RNA CASC2 restrains high glucose-induced proliferation, inflammation and fibrosis in human glomerular mesangial cells through mediating miR-135a-5p/TIMP3 axis and JNK signaling

Fig. 3

CASC2 overexpression restrains the proliferation, inflammation and fibrosis of HG-induced HMCs partly by down-regulating its target miR-135a-5p. A The expression of miR-135a-5p was determined in HMCs transfected with miR-135a-5p mimic or mimic NC by RT-qPCR. B HMCs were transfected with oe-lncRNA CASC2 alone or together with miR-135a-5p mimic. The expression of miR-135a-5p was examined by RT-qPCR. C–L HMCs transfected with oe-lncRNA CASC2 alone or together with miR-135a-5p mimic were induced by HG. C Cell proliferation was analyzed by CCK8 assay. D, E EDU assay was implemented to analyze cell proliferation ability. F, G The levels of proliferation-associated markers (PCNA and CyclinD1) were determined by Western blot assay. H–J The production of inflammation-associated cytokines was analyzed by ELISA. K, L Western blot assay was applied to measure the expression of extracellular matrix (ECM) proteins, including TGF-β1, FN and Col-4. *P < 0.05

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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