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Fig. 1 | Diabetology & Metabolic Syndrome

Fig. 1

From: Long non-coding RNA CASC2 restrains high glucose-induced proliferation, inflammation and fibrosis in human glomerular mesangial cells through mediating miR-135a-5p/TIMP3 axis and JNK signaling

Fig. 1

HG treatment promotes the proliferation, inflammation, and fibrosis of HMCs partly by reducing the level of CASC2. A The expression of CASC2 was determined in HMCs stimulated by NG (5.5 mM) or HG (25 mM) for 0 h, 12 h, 24 or 48 h by RT-qPCR. B RT-qPCR was applied to verify the overexpression efficiency of CASC2 plasmid (oe-lncRNA CASC2) in HMCs. C–L HMCs were transfected with vector or oe-lncRNA CASC2 followed by HG treatment. C CCK8 assay was applied to analyze cell proliferation ability. D, E EDU assay was performed to assess the proliferation ability of treated HMCs. F, G Western blot assay was applied to measure the expression of proliferation markers (PCNA and CyclinD1) in HMCs. H–J ELISA was conducted to evaluate the release of Mcp-1, TNF-α and IL-6 in the medium of HMCs. K, L The levels of TGF-β1, FN and Col-4 in HMCs were determined by Western blot assay. *P < 0.05

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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