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Fig. 7 | Diabetology & Metabolic Syndrome

Fig. 7

From: Circ-ACTR2 aggravates the high glucose-induced cell dysfunction of human renal mesangial cells through mediating the miR-205-5p/HMGA2 axis in diabetic nephropathy

Fig. 7

MiR-205-5p/HMGA2 axis was involved in the regulation of HG-induced DN progression. A The overexpression effect of HMGA2 transfection on the HMGA2 protein expression was analyzed using western blot. B HMGA2 protein detection was performed by western blot in control, HG, HG + miR-NC, HG + miR-205-5p, HG + miR-205-5p + pcDNA or HG + miR-205-5p + HMGA2 group. C CCK-8 was used for the examination of cell viability. D ELISA was used for the determination of IL-6 and TNF-α. E EdU assay was used for the analysis of cell proliferation. F, G Western blot was applied for the protein measurement of collagen I and collagen IV. H, I SOD activity (G) and MDA level (H) by the respective kits were applied for the assessment of oxidative stress. **P < 0.01, ***P < 0.001, ****P < 0.0001

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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