Fig. 2

Circ-FANCA knockdown promoted proliferation and suppressed apoptosis and inflammation in LPS-treated HK2 cells. a The efficiency of siRNA-mediated circ-FANCA knockdown was examined by qRT-PCR in LPS-treated HK2 cells. b Cell viability in HK2 cells treated with different concentrations of LPS for 24 h was measured by CCK-8 assay. HK2 cells were divided into 4 groups: Control, LPS, LPS + si-NC and LPS + si-circ-FANCA. c Cell viability in LPS-treated HK2 cells with different transfection or not was measured by CCK-8 assay. d, e Cell apoptosis and cell period distribution were evaluated through flow cytometry in the above groups. f The release levels of inflammatory cytokines IL-1β and TNF-α were examined via ELISA using corresponding kits. g, h The levels of SOD and MDA in HK2 cells were measured by matched kits to assess oxidative stress. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001