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Fig. 2 | Diabetology & Metabolic Syndrome

Fig. 2

From: Long non-coding RNA cancer susceptibility candidate 2 (CASC2) alleviates the high glucose-induced injury of CIHP-1 cells via regulating miR-9-5p/PPARγ axis in diabetes nephropathy

Fig. 2

CASC2 directly interacted with miR-9-5p. a The expression of miR-9-5pin CIHP-1 cells treated with normal glucose (NG), high glucose (HG) or mannitol was measured by qRT-PCR. b After CIHP-1 cells were treated with HG (mM) for 12 h, 24 h and 48 h, respectively, miR-9-5p expression was examined by qRT-PCR. c StarBase v2.0 was used to predict the target miRNAs of CASC2. d–f Dual luciferase reporter, RIP and RNA pull-down assays were utilized to assess the combination of CASC2 and miR-9-5p. g CASC2 expression in CIHP-1 cells transfected with si-NC or si-CASC2 was determined by qRT-PCR. h The expression of miR-9-5pin CIHP-1 cells transfected with si-NC, si-CASC2, Vector or CASC2 was measured using qRT-PCR analysis. *P < 0.05

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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