Fig. 2

Serum TAGE levels in STAM mice and cytotoxicity of TAGE-BSA against C2C12 cells. a Serum TAGE levels in the four stage groups of STAM mice were measured using a competitive enzyme-linked immunosorbent assay. Results were expresses as TAGE units (U)/mL of serum, with 1 U corresponding to 1.0 μg of TAGE-BSA. There were 4 mice in each group. One experiment was performed using 4 wells against the serum of one mouse to calculate the average. Data are shown as mean ± S.D. (N = 4). P-values were based on the Bonferroni test. ^**p < 0.01 vs. the no steatosis stage. b Cells were treated with 0 and 100 μg/mL non-glycated BSA and TAGE-BSA for 24 h. Cell viability was assessed by the WST-8 assay. This assay was performed in three independent experiments. One experiment was performed using 7 wells to calculate the average. Data are shown as mean ± S.D. (N = 3). P-values were based on the Bonferroni test. ^*p < 0.05 vs. the control. ^#p < 0.05 vs. the non-glycated BSA treatment