Fig. 3
qRT-PCR Confirmation of Select PI3K/Akt Genes. Relative mRNA content of protein phosphatase 2 regulatory subunit B gamma (PPP2R5C) and transcription factor A, mitochondrial (TFAM) in VAT of lean (white) and persons with obesity (gray) via qRT-PCR. Data are presented as the mean ± SD of the ΔCt (Ct target gene − Ct endogenous control) for each group. The mean ΔCt for each group is indicated by the open box within each box plot. Lower ΔCt indicates a higher expression. *p < 0.05 via 1-tailed two-sample t-test