Fig. 2
From: TCF7L2 regulates pancreatic β-cell function through PI3K/AKT signal pathway
Impact of TCF7L2 on the expressions of TCF7L2, p85, Akt and insulin secretion. MIN6 cells were transfected with TCF7L2 knockdown virus (KD1, KD3) or lenti-TCF7L2 virus for 48 h. Then the expressions of TCF7L2, p85, Akt were evaluated by western blot (a, b) and insulin concentrations in the supernatants were measured using ELISA (c). GSIS was also performed on MIN6 cells in vitro to detect the effect of TCF7L2 over-expression on insulin secretion (d) (n = 3 samples per group, *p < 0.05 and **p < 0.01 as indicated. p phosphorylated, Con control)