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Table 1 Splenocyte immunophenotypes after treatment with Pioglitazone in vitro and in vivo

From: Pioglitazone alters monocyte populations and stimulates recent thymic emigrants in the BBDZR/Wor type 2 diabetes rat model

 

In vitro

In vivo

Non-diab.

Non-diab. + Pio

Non-diab.

Non-diab. + Pio

Diabetic

Diabetic + Pio

CD3+

14.4 % ± 0.4

17.8 % ± 1.0*

86.4 % ± 2.8

86.2 % ± 0.5

10.7 % ± 3.0*

64.3 % ± 3.3*

CD3+CD4+

12.7 % ± 0.7

3.7 % ± 0.3#

53.1 % ± 10

50.0 % ± 8.8

11 % ± 8.4#

27.3 % ± 3.9#

CD3+CD4+CD25+

6.6 % ± 0.4

33.1 % ± 3.4¶

12.4 % ± 1.8

19.0 % ± 0.5

4.6 % ± 2.1¶

36.2 % ± 7.1¶

  1. Splenocytes were labeled with rat anti-CD3 AF-647, anti-CD4 PE-Cy7, and anti-CD25 FITC. Results are % ± SD, n = 3
  2. In vitro non-diabetic + Pio versus non-diabetic: * p = 0.0085, # p = 0.0006, ¶ p = 0.0022
  3. In vivo diabetic versus non-diabetic (1): * p = 0.0087, # p = 0.0043, ¶ p = 0.0040
  4. Diabetic + Pio versus Diabetic (2): * p = 0.0135, # p = 0.0302, ¶ p = 0.0178