Representative 2D-DIGE image of adipose lysates from 25-week-old Lean and CP rats. (A) The proteins (40 μg each) were labeled with Cy3 and Cy5 dyes, mixed and subjected to 2D-DIGE analysis. We selected significantly different spots of 6- and 25-week-old CP compared with age-matched control (WKY and Lean) rats. The expression level was quantified in each strain at 6 (B) and 25 weeks of age (C) and expressed as mean ± SEM of four rats per group. *P < 0.05, versus the corresponding value for WKY or Lean rats. WKY; Wistar Kyoto rats, Lean; spontaneously hypertensive rats (SHR/lean), CP; SHR/NDmcr-cp (cp/cp) rats.