Figure 5

Once daily injections of liraglutide do not affect beta-cell proliferation in corticosterone-exposed mice. Male C57Bl/6J mice were treated with 1% ethanol (vehicle, white bars) or 100 μg/ml corticosterone (GC, black bars) via the drinking water together with once-daily injections of either PBS (unstriped bars) or liraglutide (Lira) at a final dose of 0.3 mg per kg body weight (striped bars) for five consecutive weeks (n = 5-6). After treatment the pancreatic glands were dissected and processed for histology. Tissue sections were immunostained for Ki67-positive cells (A, red arrow). Morphometric analysis was used to the percentage of beta-cells that stained positive for Ki67 in the different mice cohorts (B). Images in (A) are representative pictures of Ki67 staining in the examined tissue (400 × magnification, bar represents 50 μm). Data is shown as mean ± s.e.m. A * denotes a significant (p < 0.05) effect of corticosterone treatment.