Figure 3

VCAM-1 expression in distinct brain regions were compared by measuring the OD of positive cells in brains of control and diabetic rat with immunohistochemical staining. (A). The representative image of VCAM-1 expression in the cerebral cortex of control rats. (B). The representative image of VCAM-1 expression in the cerebral cortex of diabetic rats. (C). The OD comparison of VCAM-1 expression positive cell in cerebral cortex between control and diabetic rats. VCAM-1 expression in the cerebral cortex of diabetic rats significantly increases compared with control rats (*P < 0.05). (D). The representative image of VCAM-1 expression in the thalamus of control rats. (E). The representative image of VCAM-1 expression in the thalamus of diabetic rats. (F). The OD comparison of VCAM-1 expression positive cells in thalamus between control and diabetic rats. VCAM-1 expression in the thalamus of diabetic rats significantly increases compared with control rats (*P < 0.05). (G). The representative image of VCAM-1 expression in the hippocampus of control rats. (H). The representative image of VCAM-1 expression in the hippocampus of diabetic rats. (I). The OD comparison of VCAM-1 expression positive cells in hippocampus between control and diabetic rats. VCAM-1 expression in the hippocampus of diabetic rats significantly increases compared with control rats (*P < 0.05). (J). Schematic representation indicates brain regions where VCAM-1 expression is significantly altered in diabetic rats and the rank order (from more to fewer) of expression quantity change in distinct regions of integral brain. (K). The OD comparison of positive cells between control and diabetic rats; VCAM-1 expression significantly increases in the hippocampus of diabetic rats (*P < 0.05). Bar = 100 μm.