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Table 1 Primers used for QPCR

From: Diet high in fat and sucrose induces rapid onset of obesity-related metabolic syndrome partly through rapid response of genes involved in lipogenesis, insulin signalling and inflammation in mice

Gene

Forward primer (5′ to 3′)

Reverse primer (5′ to 3′)

AMPK

AAGATCGGACACTACGTCCTG

TGCCACTTTATGGCCTGTCAA

Akt2

ACGTGGTGAATACATCAAGACC

ACCCAATGAAAGATCCATCACTC

CD36

ATGGGCTGTGATCGGAACTG

AGCCAGGACTGCACCAATAAC

CD68

GGACCCACAACTGTCACTCAT

AAGCCCCACTTTAGCTTTACC

FAS

GGAGGTGGTGATAGCCGGTAT

TGGGTAATCCATAGAGCCCAG

HMGCR

TGTTCACCGGCAACAACAAGA

CCGCGTTATCGTCAGGATGA

IRS2

TCTACACCCGAGACGAACACT

TGGGCCTTTGCCCGATTATG

LDLR

TGACTCAGACGAACAAGGCTG

ATCTAGGCAATCTCGGTCTCC

LPL

TTGCCCTAAGGACCCCTGAA

ACAGAGTCTGCTAATCCAGGAAT

LXRα

CTCAATGCCTGATGTTTCTCCT

TCCAACCCTATCCCTAAAGCAA

MAC1

ATGGACGCTGATGGCAATACC

TCCCCATTCACGTCTCCCA

MMP3

ACATGGAGACTTTGTCCCTTTTG

TTGGCTGAGTGGTAGAGTCCC

PPARγ

GGAAGACCACTCGCATTCCTT

GTAATCAGCAACCATTGGGTCA

SCD-1

TTCTTGCGATACACTCTGGTGC

CGGGATTGAATGTTCTTGTCGT

SREBP1c

GATGTGCGAACTGGACA

CATAGGGGGCGTCAAACAG

SREBP2

TGGGCGATGAGCTGACTCT

ACTGTAGCATCTCGTCGATGT

  1. AMPK : 5' adenosine monophosphate-activated protein kinase, Akt2 : protein kinase B beta, CD36 : CD36 antigen, CD68 : CD68 antigen, FAS : fatty acid synthase, HMGCR : 3-hydroxy-3-methylglutaryl-coenzyme A reductase, IRS2: insulin receptor substrate 2, LDLR : low-density lipoprotein receptor, LPL : lipoprotein lipase, LXRα : liver X receptor alpha, MAC1 : macrophage-1 antigen, MMP3 : matrix metalloprotease 3, PPARγ : peroxisome proliferator activated receptor gamma, SCD-1 : stearoyl CoA desaturase-1, SREBP : sterol regulatory element binding protein.
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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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