Effect of the HF–HS diet on hepatic genes for lipid/cholesterol metabolism and insulin signalling. (A) mRNA expression of genes related to lipid metabolism: LXRa, SREBP1c, SCD-1, FAS, PPARγ, CD36, and LPL. (B) mRNA expression of genes related to cholesterol metabolism: SREBP2, HMGCR, and LDLR. (C) mRNA expression of genes related to insulin signalling: IRS2, Akt2, and AMPK. HF–HS diet: high fat–high sucrose diet. C57BL/6 J mice were fed standard chow diet or the HF–HS diet (milk fat 21% and sucrose 34%) for 2 or 4 weeks. Values are the mean ± SE and are relative to control values, which were set at 1.0. n = 6. * P < 0.05, ** P < 0.01, *** P < 0.001.