Effect of the HF–HS diet on hepatic lipid accumulation. (A) Hepatic triglyceride and total cholesterol levels. (B) Photomicrographs of liver sections stained with hematoxylin-eosin and Oil Red O at week 2 (upper panel) and week 4 (lower panel). HF–HS diet: high fat–high sucrose diet. C57BL/6 J mice were fed standard chow diet or the HF–HS diet (milk fat 21% and sucrose 34%) for 2 or 4 weeks. Values are the mean ± SE, n = 6. ** P < 0.01, *** P < 0.001.