Figure 2

Relative expression and enzymatic activity of DDAHs in the white adipose tissue of GK rats. The extracts of the periepididymal fat pads from 11- (A–C) and 20-week-old (D–F) Wistar (open bars) and GK rats (closed bars) were analyzed for DDAH1 and DDAH2 expression, and total DDAH activity. Representative results are shown in the upper panels. Each bar in the graph represents the mean ± SEM (n = 5–10). The percentile represents the relative level of expression or activity to that of the age-matched Wistar controls. *p ≪ 0.05, **p ≪ 0.01 vs. the controls. IB, immunoblotting.