Eicosapentaenoic acid/arachidonic acid ratio and smoking status in elderly patients with type 2 diabetes mellitus

  • Kenta Okada1Email author,

    Affiliated with

    • Kazuhiko Kotani2,

      Affiliated with

      • Hiroaki Yagyu3 and

        Affiliated with

        • Shun Ishibashi1

          Affiliated with

          Diabetology & Metabolic Syndrome20146:85

          DOI: 10.1186/1758-5996-6-85

          Received: 12 June 2014

          Accepted: 8 August 2014

          Published: 13 August 2014

          Abstract

          Background

          A low ratio of eicosapentaenoic acid (EPA)/arachidonic acid (AA) is considered a risk factor for cardiovascular disease. Smoking is also a risk factor for cardiovascular disease even in an elderly population. This study investigated the relationship between EPA/AA ratio and smoking status among elderly patients with type 2 diabetes mellitus (T2DM).

          Findings

          A total of 188 elderly patients with T2DM (men/women, 114/74; mean age, 65.0 ± 7.5 years) were studied in terms of their smoking status, diabetic conditions, and blood data, including EPA and AA. Current smokers showed a lower EPA/AA ratio than non-smokers (current smokers: 0.29, n = 49; non-smokers: 0.39, n = 139, p < 0.01). This relationship remained significant after adjusting for multiple variables.

          Conclusions

          Smoking may affect the EPA/AA ratio among elderly patients with T2DM, suggesting a possible mechanism of cardiovascular disease development and indicating the importance of smoking secession in such patients.

          Keywords

          Arachidonic acid Docosahexaenoic acid Eicosapentaenoic acid Older people Smoking

          Introduction

          Cardiovascular disease (CVD) is a crucial pathology and target for preventative action in patients with diabetes mellitus (DM) [1]. The eicosapentaenoic acid (EPA)/arachidonic acid (AA) ratio in blood is considered a promising marker of myocardial infarction and cardiac death [24] with a high EPA/AA ratio predicting a low CVD risk [2, 3].

          Smoking is a major risk factor for CVD in DM [58], the adverse effects of which on the cardiovascular system can accumulate in elderly patients. Thus, in preventing CVD events, the cessation of smoking in patients aged over 50 years is considered to be equally as important as in younger patients [9]. The association between EPA/AA ratio and smoking status, especially in elderly patients with type 2 DM (T2DM) has yet to be investigated, and such information would be useful in clinical practice. The present study aimed to investigate such an association in elderly patients with T2DM [9].

          Methods

          A total of 188 patients aged over 50 years, diagnosed with T2DM (men/women, 114/74; mean age, 65.0 ± 7.5 years) and not taking medications containing EPA and AA were examined. Patients were excluded who had a history of CVD event (s), a recent acute illness, systemic inflammatory disease, severe nephropathy (i.e., stage 3 to 5), liver dysfunction, or type 1 DM. The study was approved by the Jichi Medical University Ethical Committee.

          Hypertension was determined as systolic blood pressure of ≥ 140 mmHg, diastolic blood pressure ≥90 mmHg, and/or anti-hypertensive drug use [10]. Nephropathy was defined by a urinary albumin-to-creatinine ratio of ≥ 30 mg/g creatinine [11]. Fasting blood samples were collected at our outpatient clinic in order to measure the levels of the following parameters: glucose, hemoglobin A1c (HbA1c), total cholesterol, triglyceride, high-density lipoprotein (HDL) cholesterol, EPA, docosahexaenoic acid (DHA), and AA.

          High-performance liquid chromatography (HLA-723G8; Tosoh, Tokyo, Japan) was used to measure HbA1c. Serum samples were collected in a heparinized poly-tube, lipids were extracted by Folch’s procedure, and fatty acids (tricosanoic acid (C23:0) was used as an internal standard) were methylated with boron trifluoride and methanol. EPA, DHA, and AA levels in methylated fatty acids were analyzed by gas chromatography (GC-2010; Shimadzu, Kyoto, Japan) with a capillary column (TC-70; GL Sciences Inc., Tokyo, Japan). Smoking habits were confirmed via self-reporting.

          Differences between current smokers and non-smokers were analyzed using the t-test and Chi-square test. A multivariable-adjusted analysis on the association between EPA/AA ratio and smoking status was performed using a general linear model (SPSS software, SPSS Inc., IL, USA). Parameters with skewed distributions were log-transformed in all analyses. A p-value of < 0.05 was considered to be significant.

          Results

          Current smokers were significantly younger in age, with a greater proportion of males than non-smokers (Table 1). They also exhibited a higher percentage of hypertension and neuropathy, a higher level of HbA1c, and a lower HDL cholesterol level than non-smokers. There were no significant differences in AA, DHA, and EPA levels between current smokers and non-smokers.
          Table 1

          Clinical characteristics

          Parameters

          All (n = 188)

          Non-smokers (n = 139)

          Current smokers (n = 49)

          P

          Age, years

          65.0 ± 7.5

          65.9 ± 7.4

          62.4 ± 7.3

          <0.01**

          Gender, men/women

          114/74

          71/68

          43/6

          <0.01**

          Body mass index, kg/m2

          25.5 ± 4.3

          25.6 ± 4.4

          25.2 ± 3.8

          0.59

          Hypertension, n (%)

          134 (71%)

          93 (67%)

          41 (84%)

          0.03*

          Glucose, mg/dL

          137 ± 47

          138 ± 49

          135 ± 40

          0.64

          Hemoglobin A1c, %

          7.3 ± 0.9

          7.2 ± 0.9

          7.6 ± 1.0

          0.03*

          Insulin therapy, n (%)

          55 (29%)

          41 (29%)

          14 (29%)

          0.90

          Retinopathy, n (%)

          78 (41%)

          56 (40%)

          22 (45%)

          0.57

          Neuropathy, n (%)

          107 (57%)

          73 (53%)

          34 (69%)

          0.04*

          Nephropathy, n (%)

          76 (40%)

          53 (38%)

          23 (47%)

          0.28

          LDL-cholesterol, mg/dL

          92 ± 28

          92 ± 27

          91 ± 33

          0.87

          HDL-cholesterol, mg/dL

          61 ± 16

          63 ± 16

          56 ± 16

          <0.01**

          Triglycerides, mg/dL

          107 (73-148)

          101 (67-148)

          121 (84-153)

          0.05

          Statin therapy, n (%)

          83 (44%)

          63 (45%)

          20 (41%)

          0.59

          AA, μg/mL

          173 (143-210)

          170 (141-207)

          180 (150-232)

          0.31

          DHA, μg/mL

          128 (109-176)

          129 (111-176)

          128 (98-177)

          0.15

          DHA/AA ratio

          0.78 (0.60-1.04)

          0.81 (0.62-1.07)

          0.71 (0.55-1.00)

          0.06

          EPA, μg/mL

          61 (42-101)

          62 (43-109)

          56 (34-92)

          0.06

          EPA/AA ratio

          0.37 (0.23-0.63)

          0.39 (0.24-0.65)

          0.29 (0.18-0.46)

          <0.01**

          LDL: low-density lipoprotein, HDL: high-density lipoprotein, AA: arachidonic acid, EPA: eicosapentaenoic acid, DHA: docosahexaenoic acid.

          Data are the means ± standard deviations, medians (interquartile ranges) or numbers (%).

          *p < 0.05, ** p < 0.01: comparison between current smokers and non-smokers (t-test or Chi-square test).

          Of note, a significantly lower EPA/AA ratio was demonstrated in current smokers than in non-smokers (median, 0.29 versus 0.39, p < 0.01). Weak correlations between EPA/AA ratio and other variables (age, gender, BMI, hypertension, HbA1c, insulin therapy, complications, lipids, and statin therapy) were observed for the entire population (p > 0.05 in all, data not shown). A significant difference in the EPA/AA ratio between groups (p = 0.02) remained after adjusting for multiple variables (age, gender, BMI, hypertension, HbA1c, insulin therapy, complications, lipids, and statin therapy).

          Discussion

          This study found current smokers to have a lower EPA/AA ratio than non-smokers among elderly patients with T2DM, indicating that smoking can detrimentally affect the EPA/AA balance in such patients. Considering that the EPA/AA ratio is a predictor of CVD events in patients with DM [2, 3] and smoking cessation is an achievable and effective option for preventing CVD [7, 8], the findings in this study strongly support the importance of smoking cessation in the management of CVD among elderly patients with T2DM.

          Smoking results in various oxidants that are capable of producing free radicals and lipid peroxidation [12]. Patients with T2DM are especially known to have high levels of these oxidants [13]. Polyunsaturated fatty acids, particularly omega-3 acid ethyl esters, undergo oxidation, leading to a reduction in EPA in the presence of oxidants [1416]. A previous report has indicated that AA levels in smokers (mean age, 50 years) are decreased [17], which is inconsistent with the findings of our study; however, another report indicated that AA in smokers are not decreased [18]. Of note, the metabolism of AA is not always parallel to that of EPA [1921], and compared to EPA, smoking has been shown to delay the conversion rate of AA to eicosanoids [22]. These findings may support an impairment of EPA/AA balance, namely, a decrease in the EPA/AA ratio, observed among smokers in our study. Our results should be further analyzed using additional markers, such as oxidative stress markers, in order to clarify the underlying mechanisms.

          Similar to EPA, DHA is also an omega-3 polyunsaturated fatty acid. The pathological mechanism underlying the difference between EPA and DHA is unclear. However, clinical studies have reported that lower levels of EPA, but not DHA, were significantly associated with all-cause mortality [23], and that there was no clear association between the DHA/AA ratio and cardiovascular risk [24]. The results of our study may be in line with these studies [23, 24].

          Some reports have shown that smoking worsens blood pressure [25], glycemic control [26], and neuropathic conditions [27, 28]. Similar findings were observed in our study. However, from the results of our adjusted analyses, these variables do not seem to have a major influence on the association between smoking status and EPA/AA ratio.

          This study has certain limitations. This was a cross-sectional study; thus, further intervention studies are required. Although we saw a significant difference in the association between smoking status and EPA/AA ratio, the small patient number might present a problem in terms of a lower statistical power; thus, a larger-scale study is required. Additionally, as there is no information regarding dietary fish consumption and the dose and duration of smoking (e.g., the Brinkman index), and type of cigarette were not investigated in detail, these must be included into future work.

          In summary, the present study suggests that current smoking status and a low EPA/AA ratio may enhance CVD risk in elderly patients with T2DM. Considering an individual’s smoking status coupled with EPA/AA ratio may be important in the management of T2DM, especially in the elderly. Further studies are expected.

          Authors’ information

          Kazuhiko Kotani, Hiroaki Yagyu, Shun Ishibashi are co-authors.

          Abbreviations

          CVD: 

          Cardiovascular disease

          DM: 

          Diabetes mellitus

          T2DM: 

          Type 2 diabetes mellitus

          EPA: 

          Eicosapentaenoic acid

          AA: 

          Arachidonic acid

          DHA: 

          Docosahexaenoic acid

          HbA1c: 

          Hemoglobin A1c

          HDL: 

          High-density lipoprotein.

          Declarations

          Acknowledgment

          No funding has been received for this study. The authors would like to thank the staff in our hospital.

          Authors’ Affiliations

          (1)
          Division of Endocrinology and Metabolism, Department of Internal Medicine, Jichi Medical University
          (2)
          Department of Public Health, Jichi Medical University
          (3)
          Department of Internal Medicine, University of Tsukuba Institute of Clinical Medicine

          References

          1. Ziegler D: Type 2 diabetes as an inflammatory cardiovascular disorder. Curr Mol Med. 2005, 5: 309-322. 10.2174/1566524053766095.View ArticlePubMed
          2. Matsuzaki M, Yokoyama M, Saito Y, Origasa H, Ishikawa Y, Oikawa S, Sasaki J, Hishida H, Itakura H, Kita T, Kitabatake A, Nakaya N, Sakata T, Shimada K, Shirato K, Matsuzawa Y, JELIS Investigators: Incremental effects of eicosapentaenoic acid on cardiovascular events in statin-treated patients with coronary artery disease. Circ J. 2009, 73: 1283-1290. 10.1253/circj.CJ-08-1197.View ArticlePubMed
          3. Domei T, Yokoi H, Kuramitsu S, Soga Y, Arita T, Ando K, Shirai S, Kondo K, Sakai K, Goya M, Iwabuchi M, Ueeda M, Nobuyoshi M: Ratio of serum n-3 to n-6 polyunsaturated fatty acids and the incidence of major adverse cardiac events in patients undergoing percutaneous coronary intervention. Circ J. 2012, 76: 423-429. 10.1253/circj.CJ-11-0941.View ArticlePubMed
          4. Sekikawa A, Curb JD, Ueshima H, El-Saed A, Kadowaki T, Abbott RD, Evans RW, Rodriguez BL, Okamura T, Sutton-Tyrrell K, Nakamura Y, Masaki K, Edmundowicz D, Kashiwagi A, Willcox BJ, Takamiya T, Mitsunami K, Seto TB, Murata K, White RL, Kuller LH, ERA JUMP (Electron-Beam Tomography, Risk Factor Assessment Among Japanese and U.S. Men in the Post-World War II Birth Cohort) Study Group: Marine-derived n-3 fatty acids and atherosclerosis in Japanese, Japanese-American, and white men: a cross-sectional study. J Am Coll Cardiol. 2008, 52: 417-424. 10.1016/j.jacc.2008.03.047.PubMed CentralView ArticlePubMed
          5. Suarez L, Barrett-Connor E: Interaction between cigarette smoking and diabetes mellitus in the prediction of death attributed to cardiovascular disease. Am J Epidemiol. 1984, 120: 670-675.PubMed
          6. Howard G, Wagenknecht LE, Burke GL, Diez-Roux A, Evans GW, McGovern P, Nieto FJ, Tell GS: Cigarette smoking and progression of atherosclerosis: the Atherosclerosis Risk in Communities (ARIC) Study. JAMA. 1998, 279: 119-124. 10.1001/jama.279.2.119.View ArticlePubMed
          7. Resnick HE, Foster GL, Bardsley J, Ratner RE: Achievement of American Diabetes Association clinical practice recommendations among U.S. adults with diabetes, 1999–2002: the National Health and Nutrition Examination Survey. Diabetes Care. 2006, 29: 531-537. 10.2337/diacare.29.03.06.dc05-1254.View ArticlePubMed
          8. Haire-Joshu D, Glasgow RE, Tibbs TL: American Diabetes Association: Smoking and diabetes. Diabetes Care. 2004, 27: S74-S75.View ArticlePubMed
          9. Zbikowski SM, Magnusson B, Pockey JR, Tindle HA, Weaver KE: A review of smoking cessation interventions for smokers aged 50 and older. Maturitas. 2012, 71: 131-141. 10.1016/j.maturitas.2011.11.019.View ArticlePubMed
          10. Pickering TG, Hall JE, Appel LJ, Falkner BE, Graves J, Hill MN, Jones DW, Kurtz T, Sheps SG, Roccella EJ: Subcommittee of Professional and Public Education of the American Heart Association Council on High Blood Pressure Research. Recommendations for blood pressure measurement in humans and experimental animals: Part 1: blood pressure measurement in humans: a statement for professionals from the Subcommittee of Professional and Public Education of the American Heart Association Council on High Blood Pressure Research. Hypertension. 2005, 45: 142-161. 10.1161/01.HYP.0000150859.47929.8e.View ArticlePubMed
          11. Levey AS, De Jong PE, Coresh J, El Nahas M, Astor BC, Matsushita K, Gansevoort RT, Kasiske BL, Eckardt KU: The definition, classification, and prognosis of chronic kidney disease: a KDIGO Controversies Conference report. Kidney Int. 2011, 80: 17-28. 10.1038/ki.2010.483.View ArticlePubMed
          12. Church DF, Pryor WA: Free-radical chemistry of cigarette smoke and its toxicological implications. Environ Health Perspect. 1985, 64: 111-126.PubMed CentralView ArticlePubMed
          13. Nagashima T, Oikawa S, Hirayama Y, Tokita Y, Sekikawa A, Ishigaki Y, Yamada R, Miyazawa T: Increase of serum phosphatidylcholine hydroperoxide dependent on glycemic control in type 2 diabetic patients. Diabetes Res Clin Pract. 2002, 56: 19-25. 10.1016/S0168-8227(01)00353-9.View ArticlePubMed
          14. Cohen G, Riahi Y, Sunda V, Deplano S, Chatgilialoglu C, Ferreri C, Kaiser N, Sasson S: Signaling properties of 4-hydroxyalkenals formed by lipid peroxidation in diabetes. Free Radic Biol Med. 2013, 65: 978-988.View ArticlePubMed
          15. Steinberg D, Parthasarathy S, Carew TE, Khoo JC, Witztum JL: Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity. N Engl J Med. 1989, 320: 915-924. 10.1056/NEJM198904063201407.View ArticlePubMed
          16. Cohen G, Riahi Y, Sasson S: Lipid peroxidation of poly-unsaturated fatty acids in normal and obese adipose tissues. Arch Physiol Biochem. 2011, 117: 131-139. 10.3109/13813455.2011.557387.View ArticlePubMed
          17. Simon JA, Fong J, Bernert JT, Browner WS: Relation of smoking and alcohol consumption to serum fatty acids. Am J Epidemiol. 1996, 144: 325-334. 10.1093/oxfordjournals.aje.a008933.View ArticlePubMed
          18. Belch JJ, McArdle BM, Burns P, Lowe GD, Forbes CD: The effects of acute smoking on platelet behaviour, fibrinolysis and haemorheology in habitual smokers. Thromb Haemost. 1984, 51: 6-8.PubMed
          19. Serikawa T, Miura S, Okabe M, Hongo H, Tokutome M, Yoshikawa T, Takesue K, Adachi S, Osaka K, Matsukawa R, Yanagi D, Nozoe M, Kozai T, Hironaga K, Saku K, Yamamoto Y: The ratio of eicosapentaenoic acid to arachidonic acid is a critical risk factor for acute coronary syndrome in middle-aged older patients as well as younger adult patients. J Cardiol. 2014, 63: 35-40. 10.1016/j.jjcc.2013.06.016.View ArticlePubMed
          20. Yanagisawa N, Shimada K, Miyazaki T, Kume A, Kitamura Y, Ichikawa R, Ohmura H, Kiyanagi T, Hiki M, Fukao K, Sumiyoshi K, Hirose K, Matsumori R, Takizawa H, Fujii K, Mokuno H, Inoue N, Daida H: Polyunsaturated fatty acid levels of serum and red blood cells in apparently healthy Japanese subjects living in an urban area. J Atheroscler Thromb. 2010, 17: 285-294. 10.5551/jat.2618.View ArticlePubMed
          21. Otsuka R, Kato Y, Imai T, Ando F, Shimokata H: Higher serum EPA or DHA, and lower ARA compositions with age independent fatty acid intake in Japanese aged 40 to 79. Lipids. 2013, 48: 719-727. 10.1007/s11745-013-3763-9.View ArticlePubMed
          22. Sinzinger H, Kaliman J, Oguogho A: Eicosanoid production and lymphatic responsiveness in human cigarette smokers compared with non-smokers. Lymphology. 2000, 33: 24-31.PubMed
          23. Lee SH, Shin MJ, Kim JS, Ko YG, Kang SM, Choi D, Jang Y, Chung N, Shim WH, Cho SY, Manabe I, Ha JW: Blood eicosapentaenoic acid and docosahexaenoic acid as predictors of all-cause mortality in patients with acute myocardial infarction – data from Infarction Prognosis Study (IPS) registry. Circ J. 2009, 73: 2250-2257. 10.1253/circj.CJ-09-0327.View ArticlePubMed
          24. Ninomiya T, Nagata M, Hata J, Hirakawa Y, Ozawa M, Yoshida D, Ohara T, Kishimoto H, Mukai N, Fukuhara M, Kitazono T, Kiyohara Y: Association between ratio of serum eicosapentaenoic acid to arachidonic acid and risk of cardiovascular disease: the Hisayama Study. Atherosclerosis. 2013, 231: 261-267. 10.1016/j.atherosclerosis.2013.09.023.View ArticlePubMed
          25. Groppelli A, Giorgi DM, Omboni S, Parati G, Mancia G: Persistent blood pressure increase induced by heavy smoking. J Hypertens. 1992, 10: 495-499. 10.1097/00004872-199205000-00014.View ArticlePubMed
          26. King DE, Mainous AG, Buchanan TA, Pearson WS: C-reactive protein and glycemic control in adults with diabetes. Diabetes Care. 2003, 26: 1535-1539. 10.2337/diacare.26.5.1535.View ArticlePubMed
          27. Ibrahim S, Harris ND, Radatz M, Selmi F, Rajbhandari S, Brady L, Jakubowski J, Ward JD: A new minimally invasive technique to show nerve ischaemia in diabetic neuropathy. Diabetologia. 1999, 42: 737-742. 10.1007/s001250051222.View ArticlePubMed
          28. Katulanda P, Ranasinghe P, Jayawardena R, Constantine GR, Sheriff MH, Matthews DR: The prevalence, patterns and predictors of diabetic peripheral neuropathy in a developing country. Diabetol Metab Syndr. 2012, 4: 21-10.1186/1758-5996-4-21.PubMed CentralView ArticlePubMed

          Copyright

          © Okada et al.; licensee BioMed Central Ltd. 2014

          This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​4.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://​creativecommons.​org/​publicdomain/​zero/​1.​0/​) applies to the data made available in this article, unless otherwise stated.

          Advertisement