Fig. 5

Irisin inhibited oxidative stress levels and NLRP3 expression by activating the Nrf2-TrX/TXNIP signaling axis in mice. High-fat diet + STZ-induced T2DM model mice were randomly grouped and administered irisin; the inhibitor group was injected with the Nrf2 inhibitor ML385 in vivo. (A-C) Oxidative stress levels of pancreatic tissues were detected in the mice in each group (A: ROS, B: MDA, and C: SOD). (D) WB experiments were performed to evaluate the level of expression of Nrf2, TrX, TXNIP, NLRP3, ASC, C-caspase-1, IL-1β, and GSDMD-N in pancreatic islet tissues and confirm that irisin inhibits oxidative stress and the pyroptosis of NLRP3-mediated pancreatic islet β-cells by activating the Nrf2-TrX/TXNIP signaling axis. (D) Protein semi-quantitative analysis was performed; **p < 0.01 and ***p < 0.001