Fig. 3

Effects of anagliptin on islet cell ratios and Ki67 expression in STZ-treated mice. a Islet immunohistochemical images stained with insulin or glucagon antibody in the normal controls and each of the STZ-treated groups of mice. Scale bar = 50 μm. b–d Insulin or glucagon positive area and glucagon/insulin positive area ratios (lower) of each STZ-treated group of mice were measured. e Islet immunohistochemical images stained with Ki67 or insulin antibody in each group. f Relative Ki67-insulin double positive cell area in islets from each STZ-treated mouse group. Quantitative data from 6 independent experiments are presented as bar graphs. *P < 0.05, Student’s t-test